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吴仁福, 龙晓文, 侯文杰, 潘桂平, 刘建国, 成永旭, 吴旭干. 饲料中添加雨生红球藻粉对三疣梭子蟹雌体卵巢发育、色泽、抗氧化能力和生化组成的影响[J]. 水生生物学报, 2018, 42(4): 698-708. DOI: 10.7541/2018.086
引用本文: 吴仁福, 龙晓文, 侯文杰, 潘桂平, 刘建国, 成永旭, 吴旭干. 饲料中添加雨生红球藻粉对三疣梭子蟹雌体卵巢发育、色泽、抗氧化能力和生化组成的影响[J]. 水生生物学报, 2018, 42(4): 698-708. DOI: 10.7541/2018.086
WU Ren-Fu, LONG Xiao-Wen, HOU Wen-Jie, PAN Gui-Ping, LIU Jian-Guo, CHENG Yong-Xu, WU Xu-Gan. EFFECTS OF DIETARY SUPPLEMENTATION WITH HAEMATOCOCCUS PLUVIALIS POWDER ON OVARIAN DEVELOPMENT, COLORATION, ANTIOXIDANT CAPACITY AND BIOCHEMICAL COMPOSITION OF ADULT FEMALE SWIMMING CRAB, PORTUNUSTRITUBERCULATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 698-708. DOI: 10.7541/2018.086
Citation: WU Ren-Fu, LONG Xiao-Wen, HOU Wen-Jie, PAN Gui-Ping, LIU Jian-Guo, CHENG Yong-Xu, WU Xu-Gan. EFFECTS OF DIETARY SUPPLEMENTATION WITH HAEMATOCOCCUS PLUVIALIS POWDER ON OVARIAN DEVELOPMENT, COLORATION, ANTIOXIDANT CAPACITY AND BIOCHEMICAL COMPOSITION OF ADULT FEMALE SWIMMING CRAB, PORTUNUSTRITUBERCULATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 698-708. DOI: 10.7541/2018.086

饲料中添加雨生红球藻粉对三疣梭子蟹雌体卵巢发育、色泽、抗氧化能力和生化组成的影响

EFFECTS OF DIETARY SUPPLEMENTATION WITH HAEMATOCOCCUS PLUVIALIS POWDER ON OVARIAN DEVELOPMENT, COLORATION, ANTIOXIDANT CAPACITY AND BIOCHEMICAL COMPOSITION OF ADULT FEMALE SWIMMING CRAB, PORTUNUSTRITUBERCULATUS

  • 摘要: 研究采用雨生红球藻(Haematococcus pluvialis)藻粉作为天然虾青素源, 配制4种不同虾青素含量(含量分别为0、26.60、41.62和81.37 mg/kg)的饲料(记为饲料1#—4#), 对三疣梭子蟹(Portunus trituberculatus)雌体进行为期45d的育肥实验, 研究其对雌蟹卵巢发育、色泽、抗氧化能力、免疫性能和生化组成的影响。结果表明: (1)育肥饲料中虾青素含量对雌蟹性腺指数(GSI)和肝胰腺指数(HSI)均无显著影响。(2)肝胰腺、卵巢和头胸甲中的总类胡萝卜素含量、红度(a*)值和黄度(b*)值均随饲料虾青素含量的升高而升高, 而3种组织的亮度(L*)值呈显著下降趋势(P<0.05)。(3)对抗氧化指标而言, 虾青素添加组(饲料2#—4#)血淋巴超氧化物歧化酶(SOD)和过氧化物酶(POD)活力均显著低于无虾青素组(饲料1#); 血淋巴中的总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)及肝胰腺中的SOD和T-AOC活力均随饲料中虾青素水平升高而上升, 而血淋巴和肝胰腺中的丙二醛(MDA)含量呈下降趋势。(4)对免疫指标而言, 血淋巴和肝胰腺中的酸性磷酸酶(ACP)活力均在饲料4#组最高, 而饲料1#组血淋巴中的血蓝蛋白(Hc)含量显著高于其他组(P<0.05)。(5)对生化组成而言, 肌肉和肝胰腺中的总脂、总碳水化合物及卵巢中总脂含量均随饲料中虾青素含量的升高呈上升趋势, 饲料2#组卵巢中的总碳水化合物含量最高(P<0.05)。综上所述, 三疣梭子蟹雌体育肥饲料中添加虾青素对其卵巢发育无显著影响, 但可改善色泽和提高其抗氧化能力及可食组织中的总脂和碳水化合物含量, 三疣梭子蟹雌体育肥饲料中虾青素适宜含量为50 mg/kg饲料左右。

     

    Abstract: Swimming crab (Portunus trituberculatus) is an important mariculture crab, and the light color of the pond-reared crabs may be related to low level of dietary astaxanthin. This study was designed to investigate the effects of dietary astaxanthin on ovarian development, coloration, antioxidant capacity, immune performance and biochemical composition of female P. trituberculatus. Haematococcus pluvialis powder was used as natural astaxanthin source to formulate four experimental diets with different astaxanthin levels (0, 26.60, 41.62, and 81.37 mg/kg, defined as Diet 1#—4#). The four experimental diets were fed to female P. trituberculatus for 45 days during the ovarian development; therefore, there were four treatments in this study. The results showed as follows: (1) Dietary astaxanthin had no significant effects on gonadosomatic index (GSI) and hepatosomatic index (HSI). (2) Total carotenoids content, redness (a*) and yellowness (b*) values of hepatopancreas, ovaries and carapace increased significantly with increasing dietary astaxanthin, while the lightness (L*) values of these tissues showed a decreasing trend (P<0.05). (3) For antioxidant indices, the activities of super oxide dismutase (SOD) and peroxidase (POD) in treatments (Diet 2#—4#) with supplementation of astaxanthin were significantly lower than those in Diet 1# treatment without supplementation of astaxanthin. The activities of total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) in hemolymph as well as the SOD, T-AOC in hepatopancreas increased significantly with elevated dietary astaxanthin, while a decreasing trend was found for the malondialdehyde (MDA) content in the hemolymph and hepatopancreas. (4) For the immune indices, Diet 4# treatment had the highest activity of acid phosphatase (ACP) in hemolymph and hepatopancreas, while Diet 1# treatment had the significantly higher hemolymph hemocyanin (Hc) content than other treatments (P<0.05). (5) As for the biochemical composition, the increasing trend was found on the contents of total lipids and total carbohydrate in the muscle and hepatopancreas as well as ovarian lipids with increasing dietary astaxanthin, while Diet 2# treatment had the highest total carbohydrate content in the ovaries among the four treatments (P<0.05). In conclusion, dietary supplementation of astaxanthin had no significant effect on ovarian development ofP. trituberculatus, but could improve the coloration, antioxidant capacity and the contents of total lipids and total carbohydrate in edible tissues. These results suggest the appropriate level of astaxanthin in fattening diet was approximately 50 mg/kg for adult female P. trituberculatus.

     

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