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董焕嫦, 黄春秀, 李扬. 中国沿海新报道的三种产毒拟菱形藻[J]. 水生生物学报, 2018, 42(5): 1057-1065. DOI: 10.7541/2018.130
引用本文: 董焕嫦, 黄春秀, 李扬. 中国沿海新报道的三种产毒拟菱形藻[J]. 水生生物学报, 2018, 42(5): 1057-1065. DOI: 10.7541/2018.130
DONG Huan-Chang, HUANG Chun-Xiu, LI Yang. THREE NEW KINDS OF TOXIC PSEUDO-NITZSCHIA SPECIES DERIVED FROM CHINA’S COAST[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 1057-1065. DOI: 10.7541/2018.130
Citation: DONG Huan-Chang, HUANG Chun-Xiu, LI Yang. THREE NEW KINDS OF TOXIC PSEUDO-NITZSCHIA SPECIES DERIVED FROM CHINA’S COAST[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 1057-1065. DOI: 10.7541/2018.130

中国沿海新报道的三种产毒拟菱形藻

THREE NEW KINDS OF TOXIC PSEUDO-NITZSCHIA SPECIES DERIVED FROM CHINA’S COAST

  • 摘要: 为了明确我国海域拟菱形藻属(Pseudo-nitzschia)物种的产毒特征, 从中国沿海建立了15个拟菱形藻单克隆培养株系, 利用高效液相色谱-质谱联用法对其多莫酸特征进行检测, 在10个株系中检测到多莫酸。结合光学显微镜下的群体特征和透射电镜下的超微形态学特征, 以及基于核糖体转录间隔区的分子系统学数据, 确认上述10个产毒株系分别隶属于3个物种:尖细拟菱形藻P. cuspidata、伪柔弱拟菱形藻P. pseudodelicatissima、伪善拟菱形藻P. fraudulenta, 其中伪善拟菱形藻是我国的新记录种。建立尖细拟菱形藻的11个尖细拟菱形藻株系, 其中3个株系未检出多莫酸, 其余8个株系有检出, 单细胞产毒水平为0.4—5.5 fg。建立伪柔弱拟菱形藻株系2个, 1个未检出多莫酸, 另1个株系的单细胞产毒量为1 fg。建立伪善拟菱形藻株系2个, 纯种培养株系均未检出多莫酸。利用卤虫(Artemia salina)对部分藻株进行混培诱导, 其中尖细拟菱形藻(MC4049)和伪柔弱拟菱形藻(MC3015)的产毒水平略有下降, 单细胞产毒水平分别由2、1 fg降至0.2、0.4 fg, 而伪善拟菱形藻(MC4074)的产毒能力则有显著改变, 单细胞产毒水平由未检出上升至17.5 fg。研究丰富了我国产毒拟菱形藻的物种多样性, 明确了其物种信息和产毒水平, 可为后续深入研究提供基础数据。

     

    Abstract: In order to investigate the diversity of Pseudo-nitzschia species in China’s coast and their ability in producing neurotoxin domoic acid (DA), a total of fifteen monoclonal Pseudo-nitzschia strains were isolated and established. Using a liquid chromatography tandem-mass spectrometry (LC-MS/MS) technique, DA was accordingly detected from ten monoclonal strains. Based on the morphology observed by using a light microscope (LM), a transmission electron microscope (TEM), as well as molecular analysis inferred from an internal transcribed spacer region, these fifteen strains were precisely identified which belonged to three different species involving, P. cuspidata (Hasle) Hasle, P. pseudodelicatissima (Hasle) Hasle, and P. fraudulenta(Cleve) Hasle. In addition, three out of eleven established P. cuspidata strains were nontoxic, while other eight cases were toxic with the DA abundance rate of 0.4—5.5 fg/cell. Also, one P. pseudodelicatissima strain was nontoxic, while DA production rate of 1 fg/cell was detected in another strain. Besides, no DA was tested in the two P. fraudulenta strains. Several strains were selected and their DA productions were induced by the available Artemia salina. As a result, it was shown that the ability of DA production decreased in P. cuspidate (MC4049) and P. pseudodelicatissima (MC3015) strains, from 2 and 1 to 0.2 and 0.4 fg/cell, respectively. However, the ability of DA production in P. fraudulenta(MC4074) strain swiftly increased, from an undetected level to 17.5 fg/cell. The results achieved in this study enrich the diversity of toxic Pseudo-nitzschiaspecies in China’s coast, and may establish strong research basis for conducting further studies.

     

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