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朱昶宇, 陆凯晖, 伊珍珍. 珠江浮游真核微型生物分子多样性及其与水环境的关系[J]. 水生生物学报, 2020, 44(1): 187-196. DOI: 10.7541/2020.022
引用本文: 朱昶宇, 陆凯晖, 伊珍珍. 珠江浮游真核微型生物分子多样性及其与水环境的关系[J]. 水生生物学报, 2020, 44(1): 187-196. DOI: 10.7541/2020.022
ZHU Chang-Yu, LU Kai-Hui, YI Zhen-Zhen. MOLECULAR DIVERSITIES OF PLANKOTIC MICROBIAL EUKARYOTES IN THE PEARL RIVER AND THEIR RELATIONSHIP WITH WATER ENVIRONMENT[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(1): 187-196. DOI: 10.7541/2020.022
Citation: ZHU Chang-Yu, LU Kai-Hui, YI Zhen-Zhen. MOLECULAR DIVERSITIES OF PLANKOTIC MICROBIAL EUKARYOTES IN THE PEARL RIVER AND THEIR RELATIONSHIP WITH WATER ENVIRONMENT[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(1): 187-196. DOI: 10.7541/2020.022

珠江浮游真核微型生物分子多样性及其与水环境的关系

MOLECULAR DIVERSITIES OF PLANKOTIC MICROBIAL EUKARYOTES IN THE PEARL RIVER AND THEIR RELATIONSHIP WITH WATER ENVIRONMENT

  • 摘要: 文章分别在丰水期和枯水期于珠江广州段和西江广东段43个样点采集水样, 基于末端限制性长度多态性分析, 研究了珠三角地区珠江水体的浮游真核微型生物的分子多样性及其群落结构和水体理化因子的关系。结果表明, 西江广东段及珠江广州段水体氮和磷严重超标, 水质状况堪忧。枯水期样本浮游真核微型生物香农威尔多样性指数普遍高于丰水期样本, 西江广东段样本浮游真核微型生物多样性指数普遍高于珠江广州段样本。不同水期, 不同区域的浮游真核微型生物群落结构均存在显著差异。珠江样点的浮游真核微型生物群落结构均与化学需氧量、高锰酸盐指数、氨氮、总氮、总磷高度相关; 但不同水期、不同区域的浮游真核微型生物群落结构与理化因子相关系数有所差异。此外, 分别筛选出了1个、8个末端限制性片段代表了潜在的敏感(Cystobasidium sp.或Protostelium nocturnum)、耐污(Acanthamoeba hatchettiBabesia bicornisBlastocystis sp.、Botryosphaerella sudeticaCandida caryicolaCoccomyxa simplexCryptomonas ovataFilos agilisStenophora robustaSulfonecta uniserialisTheileria sp.等)物种/类群。

     

    Abstract: To analyze the molecular diversity of planktonic microbial eukaryotes as well as relationships between the community structures and physicochemical factors based on the terminal restriction fragment length polymorphism (T-RFLP), water samples were collected from 43 sites in Guangzhou Reach of Pearl River and Guangdong Reach of Xijiang River during wet season and dry season, respectively. The results revealed that water bodies of Guangzhou Reach of Pearl River and Guangdong Reach of Xijiang River were seriously polluted by nitrogen and phosphorus, and the water were in poor quality. The diversity indexes of planktonic microbial eukaryotes in Guangdong Reach of Xijiang River were higher than those in Guangzhou Reach of Pearl River. The Shannon-Wiener indexes of samples collected in wet season were lower than those in dry season. There were significant differences in community structures of microbial eukaryotes from different seasons and regions. The community structures of planktonic microbial eukaryotes in Pearl River were affected by chemical oxygen demand, permanganate index, ammonia nitrogen, total nitrogen and total phosphorus. However, correlation coefficients between community structures and physicochemical factors were different depending on seasons and regions. In addition, one T-RF and six T-RFs were selected as possible sensitive species (Cystobasidium sp. or Protostelium nocturnum) and pollution-tolerant species (Acanthamoeba hatchetti, Babesia bicornis, Blastocystis sp., Botryosphaerella sudetica, Candida caryicola, Coccomyxa simplex, Cryptomonas ovata, Filos agilis, Stenophora robusta, Sulfonecta uniserialis and Theileria sp. etc.), respectively.

     

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