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吴晓雲, 陈叶雨, 刘亚, 赖见生, 宋明江, 龚全. 达氏鲟Elovl4、ELovl5和Elovl7克隆、组织分布及饥饿对其表达的影响[J]. 水生生物学报, 2020, 44(6): 1174-1181. DOI: 10.7541/2020.136
引用本文: 吴晓雲, 陈叶雨, 刘亚, 赖见生, 宋明江, 龚全. 达氏鲟Elovl4、ELovl5和Elovl7克隆、组织分布及饥饿对其表达的影响[J]. 水生生物学报, 2020, 44(6): 1174-1181. DOI: 10.7541/2020.136
WU Xiao-Yun, CHEN Ye-Yu, LIU Ya, LAI Jian-Sheng, SONG Ming-Jiang, GONG Quan. MOLECULAR CLONING, TISSUE DISTRIBUTION OF ELOVL4, ELOVL5 AND ELOVL7 FROM ACIPENSER DABRYANUS[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(6): 1174-1181. DOI: 10.7541/2020.136
Citation: WU Xiao-Yun, CHEN Ye-Yu, LIU Ya, LAI Jian-Sheng, SONG Ming-Jiang, GONG Quan. MOLECULAR CLONING, TISSUE DISTRIBUTION OF ELOVL4, ELOVL5 AND ELOVL7 FROM ACIPENSER DABRYANUS[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(6): 1174-1181. DOI: 10.7541/2020.136

达氏鲟Elovl4、ELovl5和Elovl7克隆、组织分布及饥饿对其表达的影响

MOLECULAR CLONING, TISSUE DISTRIBUTION OF ELOVL4, ELOVL5 AND ELOVL7 FROM ACIPENSER DABRYANUS

  • 摘要: 为研究长链脂肪酸延长酶(Elovls)作用机制, 实验根据前期达氏鲟转录组测序获得的unigene序列为基础, 得到Elovl4、Elovl5和Elovl7 CDS区序列, 并分析了3个基因在各组织及饥饿胁迫下的表达情况。结果显示, 达氏鲟Elovl4、Elovl5和Elovl7编码区为753、885和846 bp, 分别编码250、294和281个氨基酸。达氏鲟Elovl4、Elovl5和Elovl7氨基酸序列与斑点雀鳝Elovls具有较高的同源性; 组织分布结果显示, 达氏鲟Elovl4在卵巢和眼中表达最高; Elovl5在肌肉中的表达显著高于其他各个组织; Elovl7在卵巢中表达最高(P<0.05), 鳃、精巢和肌肉中表达较多。在饥饿条件下, Elovl4、Elovl5和Elovl7在肝脏中的表达量均显著下调(P<0.05), 肠道中的趋势各有不同; 饥饿3d时, Elovl4、Elovl5和Elovl7在胃中的表达量显著降低, 随后上调(P<0.05); 饥饿7d时, 脑和肌肉中Elovl4、Elovl5和Elovl7的表达量显著高于其他各组, 随后显著下降(P<0.05)。这说明Elovl4、Elovl5和Elovl7在达氏鲟营养调控过程中发挥的作用可能存在差异, 其具体作用机制需要进一步研究。

     

    Abstract: Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential for numerous critical biological processes, and elongases of very long chain fatty acids are the rate-limiting enzyme for LC-PUFAs synthesis. The CDS of Elovl4, Elovl5 and Elovl7 were cloned from the unigene database by full-length transcriptome sequencing of A. dabryanus. Results showed that the open reading frames (ORF) of Elovl4, Elovl5 and Elovl7 were 753, 885 and 846 bp which encoded 250, 294 and 281-residue peptide, respectively. Phylogenetic analysis showed that the Elovl4, Elovl5 and Elovl7 clustered with Lepisosteus oculatus. Tissue expression analysis showed that the highest Elovl4 level was in the ovary and eye; muscle Elovl5 level was significantly higher than other tissues and the highest Elovl7 was in ovary, which was followed by gill and testis (P<0.05). Starvation significantly decreased the expression of liver Elovl4, Elovl5 and Elovl7 (P<0.05). Stomach Elovl4, Elovl5 and Elovl7 reduced significantly (P<0.05) at day 3, and then significantly upregulated. The highest expression levels of Elovl4, Elovl5 and Elovl7 in brain and muscle were at day 7, and then dropped dramatically (P<0.05). These findings suggest that Elovl4, Elovl5 and Elovl7 may play certain roles in the nutritional regulation of A. dabryanus, and the specific mechanisms need further investigation.

     

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