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李海洁, 郭国军, 郭朝辉, 王林枫, 王春秀, 李明, 李国喜, 刘变枝. 饲料中添加杜仲叶粉对黄河鲤体成分、肌肉氨基酸组成和生理指标的影响[J]. 水生生物学报, 2021, 45(6): 1222-1231. DOI: 10.7541/2021.2020.295
引用本文: 李海洁, 郭国军, 郭朝辉, 王林枫, 王春秀, 李明, 李国喜, 刘变枝. 饲料中添加杜仲叶粉对黄河鲤体成分、肌肉氨基酸组成和生理指标的影响[J]. 水生生物学报, 2021, 45(6): 1222-1231. DOI: 10.7541/2021.2020.295
LI Hai-Jie, GUO Guo-Jun, GUO Chao-Hui, WANG Lin-Feng, WANG Chun-Xiu, LI Ming, LI Guo-Xi, LIU Bian-Zhi. EFFECTS OF DIETARY EUCOMMIA ULMOIDES LEAF POWDER ON BODY COMPOSITION, MUSCLE AMINO ACID COMPOSITION, PHYSIOLOGICAL INDEXES OF YELLOW RIVER CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1222-1231. DOI: 10.7541/2021.2020.295
Citation: LI Hai-Jie, GUO Guo-Jun, GUO Chao-Hui, WANG Lin-Feng, WANG Chun-Xiu, LI Ming, LI Guo-Xi, LIU Bian-Zhi. EFFECTS OF DIETARY EUCOMMIA ULMOIDES LEAF POWDER ON BODY COMPOSITION, MUSCLE AMINO ACID COMPOSITION, PHYSIOLOGICAL INDEXES OF YELLOW RIVER CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1222-1231. DOI: 10.7541/2021.2020.295

饲料中添加杜仲叶粉对黄河鲤体成分、肌肉氨基酸组成和生理指标的影响

EFFECTS OF DIETARY EUCOMMIA ULMOIDES LEAF POWDER ON BODY COMPOSITION, MUSCLE AMINO ACID COMPOSITION, PHYSIOLOGICAL INDEXES OF YELLOW RIVER CARP

  • 摘要: 试验以黄河鲤(Cyprinus carpio)为研究对象, 以饲料中杜仲(Eucommia ulmoides)叶粉0添加组为对照组, 2%、4%、6%和8%添加组为实验组, 每组3个平行, 对黄河鲤体重: (505.13±1.37) g进行55d的投喂试验, 旨在探讨杜仲叶粉在黄河鲤饲料中的应用效果。结果表明: (1)黄河鲤背肌脂肪含量随饲料中杜仲叶粉添加量升高显著下降, 粗蛋白含量显著上升(P<0.05)。除6%添加组外, 鱼体粗灰分、粗蛋白和粗脂肪鲜重含量随杜仲叶粉添加量升高呈上升趋势(P<0.05), 鱼体鲜重水分含量呈显著下降趋势(P<0.05); (2)在黄河鲤肌肉中检出16种氨基酸, 杜仲叶粉添加显著影响肌肉中苏氨酸、丝氨酸和组氨酸含量(P<0.05)。酸味氨基酸总量与总氨基酸比值在2%添加组显著高于其他处理组, 药效氨基酸总量与总氨基酸比值则在2%添加组显著低于其他处理组(P<0.05); (3)黄河鲤血浆和肝脏中GSH-PX、MDA、AKP和ACP活性随饲料中杜仲叶粉添加量增加整体呈先升高后降低趋势, 血浆和肝脏中SOD活性呈显著上升趋势(P<0.05)。血浆GSH-PX活性和MDA含量在6%处理组显著最高(P<0.05), AKP活性在4%处理组、ACP活性在8%添加组显著最高(P<0.05), SOD活性至8%处理组升至对照水平。肝脏GSH-PX活性和MDA含量在4%处理组显著最高(P<0.05), AKP活性则在6%处理组显著最高(P<0.05), ACP活性在4%处理组显著升至最高后维持在此水平(P<0.05), 肝脏SOD活性至8%处理组显著高于其他处理组(P<0.05)。综上, 综合考虑鱼体肌肉和全鱼营养成分、肌肉氨基酸组成及血液和肝脏生理指标, 推荐黄河鲤成鱼饲料中杜仲叶粉添加量在4%—6%。

     

    Abstract: To study the effects of Eucommia ulmoides leaf powder on body composition, muscle amino acid composition, physiological indicators of yellow river carp, the Eucommia ulmoides leaf powder were added into the diet at the concentrations of 0, 2%, 4%, 6% and 8%. 225 individuals with an average weight of (505.13±1.37) g were selected and fed for 55 days. The experimental data showed that: (1) Fresh drosal muscle lipid content and fresh body moisture content decreased significant while fresh drosal muscle protein and fresh body contents of crud protein, crud lipid and crud ash were significant improved with the increasing of Eucommia ulmoides leaf powder (P<0.05). (2) 16 kinds of amino acids were detected in the muscle of yellow river carp. The fresh content of Thr, Ser and His were significantly affected by the adding of Eucommia ulmoides leaf powder. The ratio of total acidic amino acids to total amino acids was significantly higher while the ratio of total pharmacodynamic amino acids to total amino acids was significantly lower in the 2% treatment compared with others (P<0.05). (3) The activities of GSH-PX, ACP, AKP and MDA content in plasma and liver significantly increased to the highest level and then decreased with the increased addition of Eucommia ulmoides leaf powder. SOD activity of Plasma and liver increased significant according with the increasing Eucommia ulmoides leaf powder (P<0.05). In plasma, the activities of GSH-PX and content of MDA were all significantly higher in 6% treatment. AKP activity showed the highest in 4% treatment while ACP activity showed the highest in 8% treatment, respectively. SOD activity increased significantly to the control level at 8% treatment. In liver, GSH-PX activity and MDA content were the highest in 4% treatment (P<0.05). AKP activity and ACP activity were the highest in 6% treatment and 4% treatment respectively (P<0.05). SOD activity showed the highest at 8% treatment (P<0.05). In conclusion, according to the fish drosal muscle and body composition, muscle amino acid, antioxidant and non-specific immune indexes in plasma and liver, the recommended level of diet Eucommia ulmoides in yellow river carp should be at 4%—6%.

     

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