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李建安, 王佳妤, 梅熠辉, 陈梅玲, 周成旭, 李亚鹤. 昼夜温差对三角褐指藻和赤潮异弯藻生长和叶绿素荧光特性的影响[J]. 水生生物学报, 2022, 46(2): 194-202. DOI: 10.7541/2021.2021.005
引用本文: 李建安, 王佳妤, 梅熠辉, 陈梅玲, 周成旭, 李亚鹤. 昼夜温差对三角褐指藻和赤潮异弯藻生长和叶绿素荧光特性的影响[J]. 水生生物学报, 2022, 46(2): 194-202. DOI: 10.7541/2021.2021.005
LI Jian-An, WANG Jia-Yu, MEI Yi-Hui, CHEN Mei-Ling, ZHOU Cheng-Xu, LI Ya-He. EFFECTS OF DIURNAL TEMPERATURE DIFFERENCE ON GROWTH AND CHLOROPHYLL FLUORESCENCE OF PHAEODACTYLUM TRICORNUTUM AND HETEROSIGMA AKASHIWO[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(2): 194-202. DOI: 10.7541/2021.2021.005
Citation: LI Jian-An, WANG Jia-Yu, MEI Yi-Hui, CHEN Mei-Ling, ZHOU Cheng-Xu, LI Ya-He. EFFECTS OF DIURNAL TEMPERATURE DIFFERENCE ON GROWTH AND CHLOROPHYLL FLUORESCENCE OF PHAEODACTYLUM TRICORNUTUM AND HETEROSIGMA AKASHIWO[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(2): 194-202. DOI: 10.7541/2021.2021.005

昼夜温差对三角褐指藻和赤潮异弯藻生长和叶绿素荧光特性的影响

EFFECTS OF DIURNAL TEMPERATURE DIFFERENCE ON GROWTH AND CHLOROPHYLL FLUORESCENCE OF PHAEODACTYLUM TRICORNUTUM AND HETEROSIGMA AKASHIWO

  • 摘要: 为研究昼夜温差对三角褐指藻和赤潮异弯藻生长的影响, 研究设置3个温度水平培养温度为22℃、18℃和22—18℃(光-暗周期), 分别记为22℃处理组、18℃处理组和22—18℃处理组对2种藻进行分批和半连续培养。结果表明: (1)在3个温度处理下, 分批培养时, 和18℃相比, 22—18℃降低了三角褐指藻在平台期的细胞密度, 但不影响其延滞期的时长。半连续培养时, 18℃处理下三角褐指藻的比生长速率最高; 而对于赤潮异弯藻, 分批培养时和22℃相比, 22—18℃和18℃处理均延长了该藻延滞期的时长, 但对其平台期细胞浓度的影响不显著, 半连续培养时该藻比生长速率在22℃和18℃处理间无差异, 而在22—18℃时显著降低; (2)在分批培养时, 和22℃相比, 低温18℃显著降低了二者的叶绿素a和类胡萝卜素含量; 半连续培养时, 温度不影响三角褐指藻的叶绿素a含量, 但昼夜温差会显著提高赤潮异弯藻类胡萝卜素的含量(和22℃相比, 增幅为24.5%); (3)无论是分批培养还是半连续培养, 温度不影响2种藻光系统Ⅱ的最大光化学效率(Fv/Fm), 但分批培养时高光600 µmol/(m2·s)条件下, 2种藻光系统Ⅱ的有效光化学效率(ФPSⅡ)和光化学猝灭(Qp)在18℃时略有增加; (4) 2种藻均在22—18℃条件下具有较高的光合固碳速率。整体来说, 三角褐指藻和赤潮异弯藻对昼夜温差的敏感性、响应和适应能力不同, 这将在一定程度上影响环境变化条件下的浮游植物群落。研究从昼夜温差的角度出发, 为深入探讨温度变化对浮游植物群落结构的影响提供了新思路。

     

    Abstract: In order to investigate the effects of diurnal temperature difference on the growth of Phaeodactylum tricornutum and Heterosigma akashiwo, we cultured these two species batch and semi-continuously at three temperature levels (18℃: 18℃ for both light and dark period; 22℃: 22℃ for both light and dark period; 22—18℃: 22℃ for the light period and 18℃ for the dark period). The results showed that: (1) Compared with 18℃, 22—18℃ reduced the cell density of P. tricornutum during batch culture at the plateau phase, but did not affect the latency time. In the semi-continuous culture, 18℃ had the highest specific growth rate (µ); for H. akashiwo, temperature had no significant effect on the cell concentration in the plateau stage, but 18℃ and 22—18℃ had prolonged latency time compared with 22℃, and 22—18℃ significantly decreased the growth rate; (2) In batch cultivation, the chlorophyll a (Chl.a) and carotenoids (Car.) content of P. tricornutum and H. akashiwo decreased significantly in 18℃ compared those in 22℃. Under semi-continuous culture, the temperature did not affect the Chl. a content, but 22—18℃ increased the Car. content of H. akashiwo by 24.5% compared to 22℃ treatment; (3) Temperature had no significant effects on the maximum effective photochemical efficiency (Fv/Fm) of photosystem II in P. tricornutum and H. akashiwo under both batch or semi-continuous culture; In batch cultivation, 18℃ showed higher effective photochemical efficiency (ФPSⅡ) and photochemical fluorescence quenching (Qp) under 600 µmol/(m2·s) conditions than other 2 conditions; (4) Both P. tricornutum and H. akashiwo showed higher carbon fixation rates at 22—18℃. Thus, this study provides a new perspective to explore the influence of temperature changes on the structure of phytoplankton community.

     

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