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姚仕彬, 任胜杰, 蔡春芳, 曹霞敏, 吴萍, 叶元土, 姜光明, 丁惠明, 张铖. 果胶高载引起的黄颡鱼绿肝症和肝纤维化与肠道菌群失调的关联[J]. 水生生物学报, 2022, 46(11): 1591-1597. DOI: 10.7541/2022.2021.0257
引用本文: 姚仕彬, 任胜杰, 蔡春芳, 曹霞敏, 吴萍, 叶元土, 姜光明, 丁惠明, 张铖. 果胶高载引起的黄颡鱼绿肝症和肝纤维化与肠道菌群失调的关联[J]. 水生生物学报, 2022, 46(11): 1591-1597. DOI: 10.7541/2022.2021.0257
YAO Shi-Bin, REN Sheng-Jie, CAI Chun-Fang, CAO Xia-Min, WU Ping, YE Yuan-Tu, JIANG Guang-Ming, DING Hui-Ming, ZHANG Cheng. GREEN LIVER SYMPTOM AND LIVER FIBROSIS CAUSED BY PECTIN OVERLOAD IN PELTEOBAGRUS FULVIDRACO MAY BE RELATED TO THE INTESTINAL DYSBACTERIOSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1591-1597. DOI: 10.7541/2022.2021.0257
Citation: YAO Shi-Bin, REN Sheng-Jie, CAI Chun-Fang, CAO Xia-Min, WU Ping, YE Yuan-Tu, JIANG Guang-Ming, DING Hui-Ming, ZHANG Cheng. GREEN LIVER SYMPTOM AND LIVER FIBROSIS CAUSED BY PECTIN OVERLOAD IN PELTEOBAGRUS FULVIDRACO MAY BE RELATED TO THE INTESTINAL DYSBACTERIOSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1591-1597. DOI: 10.7541/2022.2021.0257

果胶高载引起的黄颡鱼绿肝症和肝纤维化与肠道菌群失调的关联

GREEN LIVER SYMPTOM AND LIVER FIBROSIS CAUSED BY PECTIN OVERLOAD IN PELTEOBAGRUS FULVIDRACO MAY BE RELATED TO THE INTESTINAL DYSBACTERIOSIS

  • 摘要: 日粮中黏性膳食纤维果胶较高时可引起黄颡鱼(Pelteobagrus fulvidraco)的绿肝症和肝纤维化, 研究旨在探讨肠道菌群在上述病理过程中的潜在作用。分别配制含30%糊精和果胶的对照日粮和高果胶日粮, 命名为CON和PEC日粮, 再在CON和PEC日粮中添加0.2%的抑菌剂甲硝唑, 命名为CONM和PECM日粮。用4种日粮饲养初始体质量为(21.5±3.6) g/尾的黄颡鱼, 分别于饲养7d和56d后采样分析。16S rRNA分析结果显示, 日粮中添加甲硝唑抑制了梭杆菌门(Fusobacteria)和厚壁菌门(Firmicutes)的细菌, 使变形菌门(Proteobacteria)成为第一优势菌, 表明甲硝唑发挥了抑菌作用。饲养7d后CON组、CONM组、PEC组和PECM组的绿肝率分别为0、12%、27%和88%, 饲养56d后各组鱼肝脏纤维化程度依次增强。甲硝唑显著降低了肠道内容物中胆盐水解酶活力。肝脏中参与胆汁酸合成、转运及调控的基因表达活性与绿肝率及血清中胆汁酸含量不一致。上述结果提示肠道菌群紊乱可能是引起绿肝症和肝纤维化的重要原因, 胆汁酸可能介导了此病理过程。

     

    Abstract: The content of dietary fibers in commonly used plant feedstuffs is often more than 30%, which is the fermentation substrate for intestinal flora. With the extensive use of low fish meal feed, cultured fish species are in a state of high dietary fiber loading. However, the nutritional stress induced by dietary fiber as well as the mechanism has not received enough attention. In the previous study, we found that high viscous dietary fiber pectin induced green liver symptom and liver fibrosis in yellow catfish, Pelteobagrus fulvidraco. The purpose of this study was to explore the role of intestinal flora in the above pathological process. The diets containing 30% dextrin and pectin were named CON and PEC diets respectively. Metronidazole was then added to CON and PEC diets in a dosage of 0.2% and the obtained diets were named CONM and PECM. The four diets were fed yellow catfish with an initial weight of (21.5±3.6) g, and sampling was conducted at 7d and 56d respectively. 16s rRNA analysis showed that metronidazole inhibited bacteria of Fusobacteria and Firmicutes, resulted in Proteobacteria became the first dominant bacteria. After 7 days of feeding, the green liver rate of fish fed diet CON, CONM, PEC, PECM was 0, 12%, 27% and 88% respectively. The green liver rate of all groups was low after 56d. The degree of liver fibrosis after 56d was the highest in fish fed diet PECM, followed by PEC, and the lightest in CON. Metronidazole significantly reduced the activity of bile salt hydrolase in digesta. The serum TBA concentration in fish fed diet PEC was significantly higher than those in CON, while that in fish fed diet CONM and PECM was significantly lower than those in CON. At 56d, the serum TBA concentration in fish fed diet CONM, PEC and PECM was significantly higher than that fed CON diet. The gene expression activity involved in bile acid synthesis, transport and regulation in liver was inconsistent with the green liver rate and the serum bile acid concentration. These results indicated that dysbacteriosis was an important cause of green liver symptom and liver fibrosis induced by pectin, which might be mediated by bile acid. In view of the extensive physiological effects of intestinal flora as well as bile acids, attention should be paid to the effect of dietary fiber in aquatic feed preparation.

     

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