留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
孙艳秋, 李琪, 刘鉴毅, 庄平, 杨俊, 彭彪彪, 秦搏, 赵峰, 冯广朋, 黄晓荣. 两种饵料条件下点篮子鱼幼鱼酶活力及其消化道菌群比较[J]. 水生生物学报, 2023, 47(3): 495-504. DOI: 10.7541/2022.2021.0407
引用本文: 孙艳秋, 李琪, 刘鉴毅, 庄平, 杨俊, 彭彪彪, 秦搏, 赵峰, 冯广朋, 黄晓荣. 两种饵料条件下点篮子鱼幼鱼酶活力及其消化道菌群比较[J]. 水生生物学报, 2023, 47(3): 495-504. DOI: 10.7541/2022.2021.0407
SUN Yan-Qiu, LI Qi, LIU Jian-Yi, ZHUANG Ping, YANG Jun, PENG Biao-Biao, QIN Bo, ZHAO Feng, FENG Guang-Peng, HUANG Xiao-Rong. COMPARISON OF DIGESTIVE TRACT FLORA AND ENZYME ACTIVITY OF JUVENILE SIGANUS GUTTATUS UNDER TWO FEEDING CONDITIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 495-504. DOI: 10.7541/2022.2021.0407
Citation: SUN Yan-Qiu, LI Qi, LIU Jian-Yi, ZHUANG Ping, YANG Jun, PENG Biao-Biao, QIN Bo, ZHAO Feng, FENG Guang-Peng, HUANG Xiao-Rong. COMPARISON OF DIGESTIVE TRACT FLORA AND ENZYME ACTIVITY OF JUVENILE SIGANUS GUTTATUS UNDER TWO FEEDING CONDITIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 495-504. DOI: 10.7541/2022.2021.0407

两种饵料条件下点篮子鱼幼鱼酶活力及其消化道菌群比较

COMPARISON OF DIGESTIVE TRACT FLORA AND ENZYME ACTIVITY OF JUVENILE SIGANUS GUTTATUS UNDER TWO FEEDING CONDITIONS

  • 摘要: 以点篮子鱼(Siganus guttatus)幼鱼为研究对象, 分别用人工饲料和浒苔(Enteromorpha prolifera)进行饲喂, 采用高通量测序技术结合生化分析方法, 系统对比两种饵料对点篮子鱼 (Siganus guttatus) 幼鱼肝脏及消化道相关酶活力和消化道菌群的影响。结果显示, 浒苔组点篮子鱼幼鱼虽然生长性能低于人工饲料组, 但其淀粉酶活性更高, 点篮子鱼对浒苔有良好的摄食和消化能力, 浒苔足以满足点篮子鱼生长的需求; 此外浒苔组点篮子鱼幼鱼消化道碱性磷酸酶、超氧化物歧化酶活性均显著高于人工饲料组, 表现出了更高的免疫和抗氧化能力。在两种饵料条件下, 菌群多样性随消化道延伸呈上升趋势, 点篮子鱼幼鱼消化道菌群组成与饵料有关, 变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)为共有优势菌门; 浒苔组消化道菌群整体多样性较饲料组高, 组成结构更加丰富多样, 有利于鱼类的生长。研究结果表明点篮子鱼摄食浒苔具有良好的可行性基础, 生态价值显著, 值得深入研究与推广。

     

    Abstract: Enteromorpha prolifera is a common large-scale alga, which can make filaments grow vertically in the pond in a short time (1—2 weeks). It is a common enemy organism in filter feeding shellfish culture ponds. Once it breaks out, it will not only affect the growth of cultured varieties, but also cause biological diseases and even endanger the survival of cultured varieties, resulting in economic losses. This paper attempts to make use of the habit of Siganus guttatus feeding on Enteromorpha prolifera, and compare Enteromorpha prolifera as a natural bait with artificial feed. In order to study the structure of digestive tract flora, digestive enzyme and nonspecific immune enzyme activity of juvenile Siganus guttatus were fed with artificial feed and Enteromorpha prolifera respectively. High-throughput sequencing technology combined with biochemical analysis methods were used to systematically analyze and compare the composition and distribution of microflora in the stomach, pyloric caecum and intestine of juvenile Siganus guttatus, and to compare the influence on growth performance and digestive enzyme activity of the juvenile Siganus guttatus with the two diets. Biochemical analysis showed that although the growth performance of juvenile Siganus guttatus fed with Enteromorpha prolifera was lower than that of the artificial feed group, its amylase activity was higher than that of the artificial feed group. The juvenile Siganus guttatus had good feeding and digestive ability to Enteromorpha prolifera, which was enough to meet the growth needs of Siganus guttatus. The activities of alkaline phosphatase and superoxide dismutase in the digestive tract of Enteromorpha prolifera group were significantly higher than those in the artificial diet group, showing higher immune and antioxidant capacity. High throughput sequencing results showed that bacterial diversity shows an upward trend with the extension of the digestive tract. The dominant bacteria in the digestive tract were Proteobacteria, Firmicutes and Bacteroidetes. The composition of bacteria in the digestive tract of Siganus guttatus was greatly affected by the diet. The results can provide data support for the bait research of Siganus guttatus. At the same time, it shows that the feeding of Siganus guttatus on Enteromorpha prolifera has a good feasibility basis and significant ecological value, which is worthy of in-depth research and promotion.

     

/

返回文章
返回