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冯丹, 谭艾娟, 吕世明, 郑萌盟, 杨贵利. 贵州省浮萍形态学鉴定与遗传多样性分析[J]. 水生生物学报, 2022, 46(7): 974-982. DOI: 10.7541/2022.2021.097
引用本文: 冯丹, 谭艾娟, 吕世明, 郑萌盟, 杨贵利. 贵州省浮萍形态学鉴定与遗传多样性分析[J]. 水生生物学报, 2022, 46(7): 974-982. DOI: 10.7541/2022.2021.097
FENG Dan, TAN Ai-Juan, LÜ Shi-Ming, ZHENG Meng-Meng, YANG Gui-Li. MORPHOLOGICAL IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF DUCKWEEDS IN GUIZHOU PROVINCE[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(7): 974-982. DOI: 10.7541/2022.2021.097
Citation: FENG Dan, TAN Ai-Juan, LÜ Shi-Ming, ZHENG Meng-Meng, YANG Gui-Li. MORPHOLOGICAL IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF DUCKWEEDS IN GUIZHOU PROVINCE[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(7): 974-982. DOI: 10.7541/2022.2021.097

贵州省浮萍形态学鉴定与遗传多样性分析

MORPHOLOGICAL IDENTIFICATION AND GENETIC DIVERSITY ANALYSIS OF DUCKWEEDS IN GUIZHOU PROVINCE

  • 摘要: 为调查贵州省浮萍种间亲缘关系及变异情况, 研究在贵州省辖9个地级行政区的多处水环境中共采集到 41份浮萍种质, 通过形态鉴定这些种质中33个属于绿萍属(Lemna), 6个属于紫萍属(Spirodela), 2个属于斑萍属/兰氏萍属(Landoltia)。利用叶绿体atpF-atpH间隔序列和rpS16内含子序列进行分子生物学分析和鉴定, 41份浮萍种质聚类到Lemna aequinoctialis、Lemna minor、Spirodela polyrhizaLandoltia punctata 4个种。atpF-atpH间隔序列和rpS16内含子序列的单倍型多样性分别为0.98700和0.64700, 群体突变率分别为0.15380和0.14334, 平均每kb核苷酸差异数为47.20000和61.72200, 核苷酸多态性分别为0.08725和0.09158。研究讨论了中国亚热带温润季风地区浮萍的遗传多样性, 为浮萍亲缘关系分析提供了依据, 也为亚热带湿润季风气候地区植物的种属鉴定及资源化利用奠定了基础。

     

    Abstract: Duckweed, a small floating aquatic plant, belongs to the monocotyledonous family of Lemnaceae. Duckweed consists of 5 genera and 36 species with a wide distribution in the natural environment. The genetic diversity of duckweed affects adaptability to different natural environments. which is important to evaluate the evolutionary potential and survival pressure of species. Guizhou Province has a subtropical humid monsoon climate area with rich duckweed germplasm resources. To explore the evolutionary potential and survival pressure of duckweed, 41 duckweed ecotypes were collected from 9 cities in Guizhou Province for genetic diversity analysis. The chloroplast atpF-atpH spacer sequence and rpS16 intron sequence were used for molecular biological analysis, and NJ phylogenetic Tree was constructed by MEGA 6.0 software to determine the species classification of samples. Morphological identification showed that 33 ecotypes belong to Lemna, 6 ecotypes belong to Spirodela, and 2 ecotypes belong to Landoltia. Molecular biological showed that 41 ecotypes were clustered into 4 species, including Lemna aequinoctialis, Lemna minor, Spirodela polyrhiza, and Landoltia punctata. The haplotype diversity of the atpF-atpH spacer sequences and rpS16 intron sequences were 0.98700 and 0.64700, the population mutation rates were 0.15380 and 0.14334, nucleotide differences per Kb were 47.20000 and 61.72200 and nucleotide diversity were 0.08725 and 0.09158, respectively. The results provide a basis for genetic relationship analysis of duckweed, and lay a foundation for species identification and resource utilization of plants in subtropical humid monsoon climate area.

     

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