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陈凯, 方成池, 吴志刚, 熊凡, 俞丹, 崔永德, 张琪, 王宝强, 姜传奇, 宋立荣, 王洪铸, 刘焕章, 陈晓飞, 凌海波, 蔡俊雄, 李涛, 何舜平, 缪炜, 熊杰, 曾宏辉. AeDNA: 水生生物eDNA数据库[J]. 水生生物学报, 2022, 46(11): 1741-1747. DOI: 10.7541/2022.2022.0379
引用本文: 陈凯, 方成池, 吴志刚, 熊凡, 俞丹, 崔永德, 张琪, 王宝强, 姜传奇, 宋立荣, 王洪铸, 刘焕章, 陈晓飞, 凌海波, 蔡俊雄, 李涛, 何舜平, 缪炜, 熊杰, 曾宏辉. AeDNA: 水生生物eDNA数据库[J]. 水生生物学报, 2022, 46(11): 1741-1747. DOI: 10.7541/2022.2022.0379
CHEN Kai, FANG Cheng-Chi, WU Zhi-Gang, XIONG Fan, YU Dan, CUI Yong-De, ZHANG Qi, WANG Bao-Qiang, JIANG Chuan-Qi, SONG Li-Rong, WANG Hong-Zhu, LIU Huan-Zhang, CHEN Xiao-Fei, LING Hai-Bo, CAI Jun-Xiong, LI Tao, HE Shun-Ping, MIAO Wei, XIONG Jie, ZENG Hong-Hui. AeDNA: AQUATIC ENVIRONMENTAL DNA DATABASE[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1741-1747. DOI: 10.7541/2022.2022.0379
Citation: CHEN Kai, FANG Cheng-Chi, WU Zhi-Gang, XIONG Fan, YU Dan, CUI Yong-De, ZHANG Qi, WANG Bao-Qiang, JIANG Chuan-Qi, SONG Li-Rong, WANG Hong-Zhu, LIU Huan-Zhang, CHEN Xiao-Fei, LING Hai-Bo, CAI Jun-Xiong, LI Tao, HE Shun-Ping, MIAO Wei, XIONG Jie, ZENG Hong-Hui. AeDNA: AQUATIC ENVIRONMENTAL DNA DATABASE[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1741-1747. DOI: 10.7541/2022.2022.0379

AeDNA: 水生生物eDNA数据库

  • 摘要: 环境 DNA (eDNA) 技术是一种生态和生物多样性监测和评价的新手段, 完整和准确的参考序列库是eDNA技术应用于水生生物多样性调查的基础。当前, 不同水生生物eDNA参考序列还存在诸多问题, 如不同类群使用的标记基因不同且资源较为分散, 部分参考序列分类不准确, 以及针对我国各类水体中水生生物eDNA参考序列不多等。针对上述问题, 研究构建了水生生物eDNA数据库(AeDNA, http://aedna.ihb.ac.cn/)。 AeDNA整合了DNA条形码和基因组两种类型参考序列。其中18S、28S、ITS、COΙ、12S、rbcL 等各类DNA条形码60余万条, 涉及2万余种鱼类、1万余种水生植物、1万余种底栖动物、1万余种浮游动物和1万余种浮游植物; 基因组包含线粒体、叶绿体等细胞器基因组6199个及万种鱼类基因组计划和万种原生生物基因组计划所产生的物种基因组。涉及的生境有江、河、湖、海、冰川和温泉等各类水环境, 尤其数据库构建团队贡献的6万余条参考序列, 具有我国丰富的各类水体生境信息。总体来说, AeDNA是一个数据量大、类群覆盖全、准确性高且具有我国水生生物特色的综合性eDNA参考序列库, 是水生态和水生生物多样性监测的重要基础资源。

     

    Abstract: Environmental DNA (eDNA) technology is an emerging method for ecological and biodiversity monitoring and evaluation, and is one of the frontier technologies. A complete and accurate reference sequence database is the basis of the application of eDNA technology to the investigation of aquatic biodiversity. At present, there are still some problems in eDNA reference sequences of different aquatic organisms, such as the use of different marker genes for different groups of aquatic organisms, the scattered distribution and inaccurate classification of reference sequences, and the lacking of reference sequences for aquatic organisms in China. To address these issues, the Aquatic eDNA database (AeDNA, http://aedna.ihb.ac.cn/) was constructed in this study. AeDNA integrates two types of reference sequences: DNA barcode and genome database. The DNA barcode database contains more than 600000 records of different marker genes, including 18S, 28S, ITS, CO1, 12S and rbcL, etc., which covers about 20000, 10000, 10000, 10000 and 10000 species of fishes, plants, Zoobenthos, zooplankton and phytoplankton, respectively. The genome database contains 6199 organelle genomes of mitochondria and chloroplast, as well as the genomes of fish and protist which generated by the 10000 Fish Genomes Project and the Protist 10000 Genomes Project. The habitats cover various water environments such as rivers, lakes, seas, glaciers and hot springs. In particular, AeDNA contains more than 60000 reference sequences of aquatic organisms in various water environments of China. In summary, AeDNA is a comprehensive eDNA reference sequence database with large amount of data, high coverage of taxa, high accuracy and more data for aquatic organisms in China. It will be a fundamental resource for aquatic ecology and biodiversity monitoring.

     

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