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黄莹, 郭雅哲, 娄格格, 满洲, 葛汝祥, 朱晓鸣, 向勇, 陈新华. 饲料中黄曲霉毒素B1对克氏原螯虾幼虾生长、饲料利用和肝胰腺组织结构的影响[J]. 水生生物学报, 2023, 47(2): 279-289. DOI: 10.7541/2023.2022.0260
引用本文: 黄莹, 郭雅哲, 娄格格, 满洲, 葛汝祥, 朱晓鸣, 向勇, 陈新华. 饲料中黄曲霉毒素B1对克氏原螯虾幼虾生长、饲料利用和肝胰腺组织结构的影响[J]. 水生生物学报, 2023, 47(2): 279-289. DOI: 10.7541/2023.2022.0260
HUANG Ying, GUO Ya-Zhe, LOU Ge-Ge, MAN Zhou, GE Ru-Xiang, ZHU Xiao-Ming, XIANG Yong, CHEN Xin-Hua. DIETARY AFLATOXIN B1 ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 279-289. DOI: 10.7541/2023.2022.0260
Citation: HUANG Ying, GUO Ya-Zhe, LOU Ge-Ge, MAN Zhou, GE Ru-Xiang, ZHU Xiao-Ming, XIANG Yong, CHEN Xin-Hua. DIETARY AFLATOXIN B1 ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 279-289. DOI: 10.7541/2023.2022.0260

饲料中黄曲霉毒素B1对克氏原螯虾幼虾生长、饲料利用和肝胰腺组织结构的影响

DIETARY AFLATOXIN B1 ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)

  • 摘要: 以含不同浓度黄曲霉毒素B1(AFB1)(0、10、100和1000 μg/kg饲料)的4种饲料饲喂初始均重为(0.382±0.005) g的克氏原螯虾(Procambarus clarkii)幼虾42d, 探讨AFB1对克氏原螯虾幼虾生长性能、饲料效率和肝胰腺组织结构的影响。结果显示, 100和1000 μg/kg毒素组幼虾的存活率、摄食率、终末体重、特定生长率和饲料效率均显著低于对照组, 10 μg/kg毒素组与对照组无显著差异。10 μg/kg毒素组幼虾肝胰腺碱性磷酸酶(AKP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽转移酶(GST)活性和丙二醛(MDA)含量均与对照组无显著差异, 超氧化物歧化酶(SOD)活性显著低于对照组。饲料AFB1含量≥100 μg/kg时显著影响了克氏原螯虾幼虾上述肝胰腺酶的活性。10 μg/kg 毒素组肝胰腺组织结构发生轻微变化, 100和1000 μg/kg毒素组幼虾的肝胰腺表现出严重病变, R细胞数量减少而B细胞数量增多。超微结构结果显示, 随着AFB1浓度的增加, 肝胰腺细胞内线粒体肿胀, 粗面内质网扩张, 囊泡增多, 脂滴变大。饲料中AFB1≤100 μg/kg时克氏原螯虾幼虾体内均未检测出AFB1。1000 μg/kg毒素组幼虾虾体中AFB1积累量为1.65 μg/kg, 未超过FDA食品安全限定标准。综上所述, 饲料中AFB1含量≥10 μg/kg时即对克氏原螯虾幼虾产生不良影响, 说明克氏原螯虾幼虾对AFB1较为敏感。

     

    Abstract: To evaluate the effects of dietary aflatoxin B1 (AFB1) on growth performance, feed efficiency, and histological changes in juvenile red swamp crayfish (Procambarus clarkii), crayfish with initial body weight of (0.382±0.005) g were fed with four diets containing 0, 10, 100 and 1000 μg/kg AFB1 for a 42-day trial. Significant lower survival rate (SR), feeding rate (FR), final body weight (FBW), specific growth rate (SGR) and feed efficiency (FE) were observed in 100 μg/kg and 1000 μg/kg AFB1 group, while no significant differences were found in 10 μg/kg group compared to control group. There were no significant differences in alkaline phosphatase (AKP), alanine aminotransferase (ALT), aspartate, aminotransferase (AST), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and malondialdehyde (MDA) in hepatopancreas between the control and 10 μg/kg AFB1 group, while significant decrease in superoxide dismutase (SOD) was observed in 10 μg/kg AFB1 group. AFB1 above 100 μg/kg significantly impacted activities of the aforementioned hepatopancreas enzymes. Slight histological changes were identified in hepatopancreas of 10 μg/kg AFB1 group. However, severe lesions of hepatopancreas were found in 100 μg/kg and 1000 μg/kg AFB1 group, which showed decreased numbers of R-cells and increased numbers of B-cells. The ultrastructural results showed that with the increase of AFB1 concentration, hepatopancreas demonstrated swelling mitochondria, expanded rough endoplasmic reticulum, increased cytoplasmic vesicles and larger lipid droplets. No AFB1 residues were detected in whole body when crayfish were fed with AFB1 up to 100 μg/kg, however, tiny dose (1.65 μg/kg) of AFB1 were detected in the group with 1000 μg/kg AFB1, which was below the safety limitation of FDA. In summary, dietary AFB1 above 10 μg/kg had an adverse effect on juvenile crayfish, which indicated that red swamp crayfish was a sensitive species to AFB1.

     

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