留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
雷姚, 周春花, 欧阳珊, 吴小平. 不同环境样本类型对蚌类环境DNA监测的差异研究[J]. 水生生物学报, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266
引用本文: 雷姚, 周春花, 欧阳珊, 吴小平. 不同环境样本类型对蚌类环境DNA监测的差异研究[J]. 水生生物学报, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266
LEI Yao, ZHOU Chun-Hua, OUYANG Shan, WU Xiao-Ping. DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266
Citation: LEI Yao, ZHOU Chun-Hua, OUYANG Shan, WU Xiao-Ping. DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266

不同环境样本类型对蚌类环境DNA监测的差异研究

DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES

  • 摘要: 为了阐明在使用环境DNA宏条形码技术时不同环境样本类型如何影响蚌类物种的可检测性, 于2021年冬季和春季在鄱阳湖分别采集表层水、底层水和沉积物进行环境DNA宏条形码分析, 并结合传统方法采集验证。基于环境DNA宏条形码技术共检测到鄱阳湖蚌类33种, 传统方法共采集蚌类18种, 环境DNA宏条形码技术能检测出传统方法采集到的所有蚌类物种。表层水和底层水注释到的蚌类物种数均分别高于沉积物的, 且表层水和底层水注释到的蚌类物种分别完全覆盖沉积物的。基于环境DNA宏条形码技术的蚌类α多样性水平季节差异不显著, 但蚌类β多样性水平季节差异显著。表层水和底层水的蚌类多样性均显著高于沉积物样本的, Beta多样性分析也显示水体样本(表层水和底层水分别)和沉积物样本存在显著性差异。但表层水和底层水的蚌类多样性和群落结构均无显著差异。鄱阳湖蚌类群落结构与环境因子的关联分析表明水深(WD)、透明度(SD)、水温(WT)和总氮(TN)显著影响蚌类群落结构。环境DNA宏条形码技术在蚌类的多样性监测中可行, 且采水样比采沉积物效果好, 表层水和底层水无显著差异。

     

    Abstract: In order to clarify how different environmental sample types affect the detectability of mussel species when using environmental DNA metabarcoding technology, surface water, bottom water and sediment were collected in Poyang Lake in winter and spring of 2021 for environmental DNA metabarcoding analysis, and then combined with traditional methods for collection and verification. A total of 33 species of mussels from Poyang Lake were detected by environmental DNA metabarcoding technology, while 18 species were collected by traditional methods. All the species collected by traditional methods could be detected by environmental DNA metabarcoding technology. The number of mussel species annotated in surface water and bottom water was respectively higher than that in sediment, and the mussel species annotated in surface water and bottom water completely covered sediment, respectively. There was no significant seasonal difference in α diversity level of mussels based on environmental DNA metabarcoding, but significant seasonal difference in β diversity level of mussels. The mussel diversity in both surface and bottom water was significantly respectively higher than that in sediment samples. The Beta diversity analysis also showed significant differences between water samples (surface and bottom water respectively) and sediment samples. But there were no significant differences in the diversity and community structure between surface and bottom water. Water depth (WD), depth of Secchi disk (SD), water temperature (WT) and total nitrogen (TN) significantly affected the community structure of mussels in Poyang Lake. Environmental DNA metabarcoding is feasible in monitoring mussel freshwater diversity, and water sampling is better than sediment sampling. There is no significant difference between surface water and bottom water.

     

/

返回文章
返回