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张琪, 夏逸若, 李林, 李天丽, 郑凌凌, 宋立荣. 淡水藻种库(FACHB)库藏产2-MIB蓝藻的鉴定及其产嗅特征研究[J]. 水生生物学报, 2023, 47(7): 1115-1128. DOI: 10.7541/2023.2022.0270
引用本文: 张琪, 夏逸若, 李林, 李天丽, 郑凌凌, 宋立荣. 淡水藻种库(FACHB)库藏产2-MIB蓝藻的鉴定及其产嗅特征研究[J]. 水生生物学报, 2023, 47(7): 1115-1128. DOI: 10.7541/2023.2022.0270
ZHANG Qi, XIA Yi-Ruo, LI Lin, LI Tian-Li, ZHENG Ling-Ling, SONG Li-Rong. IDENTIFICATION AND CHARACTERIZATION OF 2-MIB-PRODUCING CYANOBACTERIA IN FACHB[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(7): 1115-1128. DOI: 10.7541/2023.2022.0270
Citation: ZHANG Qi, XIA Yi-Ruo, LI Lin, LI Tian-Li, ZHENG Ling-Ling, SONG Li-Rong. IDENTIFICATION AND CHARACTERIZATION OF 2-MIB-PRODUCING CYANOBACTERIA IN FACHB[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(7): 1115-1128. DOI: 10.7541/2023.2022.0270

淡水藻种库(FACHB)库藏产2-MIB蓝藻的鉴定及其产嗅特征研究

IDENTIFICATION AND CHARACTERIZATION OF 2-MIB-PRODUCING CYANOBACTERIA IN FACHB

  • 摘要: 为认识产二甲基异莰醇(2-MIB)蓝藻的形态和产嗅特征, 从国家水生生物种质资源库淡水藻种库中筛选出24株可产2-MIB藻株, 描述了这些藻株的形态特征和生境分布。结合形态和16S rRNA基因分析对藻株进行物种鉴定复核, 修订了部分库藏藻株物种名称, 例如发现库藏产2-MIB的浮丝藻属种类应当被重新鉴定为拉氏拟浮丝藻或索状气丝藻。基于mic基因系统发育树分析, 显示蓝藻mic基因形成5个分支。通过2-MIB含量检测发现, 不同藻株间单个细胞总2-MIB含量为6—2549 fg/cell, 其含量通常为拉氏拟浮丝藻>索状气丝藻>灰假鱼腥藻。研究提供了产2-MIB蓝藻的形态、分子、生态和产嗅特性等的基础数据, 首次报道气丝藻、沙丝藻、苏打丝藻种类可产2-MIB, 并在国内首次报道了产2-MIB的微鞘藻, 为进一步研究产2-MIB蓝藻生理生态特性提供重要的实验材料和科学依据。

     

    Abstract: Odor problems in freshwater caused by 2-methylisoborneol (2-MIB) have received much attention in China recently. The odorous 2-MIB in freshwater is known to be mainly produced by a group of filamentous cyanobacteria which cause offensive taste and odor in drinking water and fish catch. From the detection of MIB synthase gene and GC/MS analyses, 24 strains of 2-MIB-producing cyanobacteria were detected in Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB-collection), National Aquatic Biological Resource Center, the largest microalgal culture collection in China. These strains were re-identified as Planktothricoides raciborskii (Wołoszyńska) Suda & Watanabe, Aerosakkonema funiforme Thu & Watanabe, Pseudanabaena cinerea Tuji & Niiyama, Oscillatoria lutea var. contorta Baker et Bold, Microcoleus sp., Desertifilum tharense Dadheech & Krienitz and Sodalinema sp. by morphological descriptions and molecular characteristics based on the 16S rRNA gene, respectively. Most 2-MIB-producing strains of Planktothrix in FACHB-collection should be re-identified as P. raciborskii or A. funiforme. This was the first report of 2-MIB-producing filamentous cyanobacteria of Aerosakkonema, Desertifilum and Sodalinema in the world, and was also the first report of 2-MIB-producing Microcoleus in China. The phylogenetic analyses base on 16S rRNA gene indicated that 2-MIB-producing strains in FACHB-collection were placed on seven separated clades, which reflected true taxonomic relationships. The genera Planktothricoides, Aerosakkonema, Pseudanabaena, Microcoleus, Desertifilum and Sodalinema formed six monophyletic clades in 16S rDNA tree; however, O. lutea var. contorta was closely related to some strains of Oscillatoria, Phormidium and Kamptonema. The phylogenetic analyses based on mic gene indicated that 2-MIB-producing cyanobacteria strains formed five clades with high support values. 2-MIB-producing P. raciborskii and A. funiforme were clustered into Clade I in mic tree. Two 2-MIB-producing Microcoleus strains were placed on Clade Ⅱ and Ⅲ, respectively. All 2-MIB-producing Pseudanabaena strains were clustered into Clade Ⅳ. Phylogenetic topology of some 2-MIB-producing strains in mic tree were incongruent with the topology in 16S rDNA tree. Cell quota of 2-MIB produced by these strains may vary in a wide range, varying from 6—2549 fg/cell. Cell quota of 2-MIB varied considerably among species, such as P. raciborskii > A. funiforme > P. cinerea. The results provide important experimental materials and basic data on morphological, molecular, ecological and 2-MIB-producing characteristics of filamentous cyanobacteria, and contribute to understanding ecophysiological characteristics 2-MIB-producing cyanobacteria.

     

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