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何桂伦, 谢明胜, 屈康渊, 陈欣, 朱文博, 陈政榜, 谭北平, 谢诗玮. 两种不同复合诱食剂添加水平的饲料中添加甜菜碱对凡纳滨对虾生长性能、饲料利用、脂质代谢和免疫应答的影响[J]. 水生生物学报, 2023, 47(9): 1446-1455. DOI: 10.7541/2023.2022.0464
引用本文: 何桂伦, 谢明胜, 屈康渊, 陈欣, 朱文博, 陈政榜, 谭北平, 谢诗玮. 两种不同复合诱食剂添加水平的饲料中添加甜菜碱对凡纳滨对虾生长性能、饲料利用、脂质代谢和免疫应答的影响[J]. 水生生物学报, 2023, 47(9): 1446-1455. DOI: 10.7541/2023.2022.0464
HE Gui-Lun, XIE Ming-Sheng, QU Kang-Yuan, CHEN Xin, ZHU Wen-Bo, CHEN Zheng-Bang, TAN Bei-Ping, XIE Shi-Wei. TWO DIETARY BETAINE SUPPLEMENTATIONS ON GROWTH, FEED UTILIZATION, LIPID METABOLISM, AND IMMUNE RESPONSE OF WHITE SHRIMP (LITOPENAEUS VANNAMEI) FED TWO LEVELS OF COMPOUND ATTRACTANTS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1446-1455. DOI: 10.7541/2023.2022.0464
Citation: HE Gui-Lun, XIE Ming-Sheng, QU Kang-Yuan, CHEN Xin, ZHU Wen-Bo, CHEN Zheng-Bang, TAN Bei-Ping, XIE Shi-Wei. TWO DIETARY BETAINE SUPPLEMENTATIONS ON GROWTH, FEED UTILIZATION, LIPID METABOLISM, AND IMMUNE RESPONSE OF WHITE SHRIMP (LITOPENAEUS VANNAMEI) FED TWO LEVELS OF COMPOUND ATTRACTANTS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1446-1455. DOI: 10.7541/2023.2022.0464

两种不同复合诱食剂添加水平的饲料中添加甜菜碱对凡纳滨对虾生长性能、饲料利用、脂质代谢和免疫应答的影响

TWO DIETARY BETAINE SUPPLEMENTATIONS ON GROWTH, FEED UTILIZATION, LIPID METABOLISM, AND IMMUNE RESPONSE OF WHITE SHRIMP (LITOPENAEUS VANNAMEI) FED TWO LEVELS OF COMPOUND ATTRACTANTS

  • 摘要: 实验旨在研究两种含不同水平复合诱食剂(8%, 0)的基础饲料中添加甜菜碱对凡纳滨对虾(Litopenaeus vannamei)生长性能、饲料利用率、脂质代谢和免疫应答的影响。共制备原料型复合诱食剂对照组(P)、豆粕替代原料型诱食剂组(N)及在二者基础上分别添加0.6%甜菜碱的PB组和NB组等四组饲料, 喂养幼虾初始体重(0.71±0.00) g 7周。结果显示, 饲料中添加原料型复合诱食剂可提高对虾肝胰腺中fasaccsoddorsalrelish及肠道tollsod的表达量, 而肠道中relish表达量则相反。饲料中添加甜菜碱可提高对虾肌肉灰分含量, 上调对虾肝胰腺中fasaccsoddorsal及肠道中sod的表达量, 而降低摄食量、肌肉粗脂肪和血淋巴中低密度脂蛋白胆固醇和丙二醛的含量及肝胰腺总一氧化氮合酶活性, 下调肝胰腺中acoxcpt-1myd88relish的表达量。原料型复合诱食剂与甜菜碱在对虾的饲料系数、肝胰腺总一氧化氮合酶活力、ampkacccpt-1sodmyd88relish表达量等指标上呈现显著的交互作用。由此可见, 饲料中添加1.30%酵母提取物+ 4.80%鱿鱼内脏粉+ 1.90%鱼溶浆提高了对虾的免疫力和抗氧化能力。饲料中添加0.6%甜菜碱降低了对虾摄食量, 促进了脂质利用, 并上调了免疫相关基因的表达。

     

    Abstract: Feed attractants have been widely used to improve the palatability of aquatic animal feeds, but there are few studies on the effects of feed attractants on growth and health of Litopenaeus vannamei. This study aims to investigate the effects of betaine supplementations on growth performance, feed utilization, lipid metabolism, and immune response of Litopenaeus vannamei fed two levels of raw feed attractants (8%, 0). Juvenile shrimp initial body weight (0.71±0.00) g were randomly distributed into four groups, each group included four fiberglass tanks 30 shrimp in each tank (0.3 m³). The shrimp were fed with raw feed attractants group (P), soybean meal replacing feed attractants in P (N), raw feed attractants + betaine group (PB) and betaine supplementation group (NB) for 7 weeks, respectively. Results showed that the raw feed attractants enhanced the expression levels offas, acc, sod, dorsal, relish in hepatopancreas, as well as toll and sod in the intestine of shrimp, while the expression levels of relish in the intestine were reversed. Betaine increased ash content in muscle, upregulated fas,acc, sod, and dorsal in hepatopancreas, as well as sod expression in the intestine, while it decreased feed intake, muscle crude lipid, low density lipoprotein cholesterol, malondialdehyde contents and total nitric oxide synthase activity in hepatopancreas, as well as the expression levels of acox, cpt-1,myd88 and relish in hepatopancreas. The interaction of raw feed attractants and betaine had significant effects on feed conversion ratio, hepatopancreas total nitric oxide synthase activity, ampk, acc, cpt-1, sod, myd88 and relish. In conclusion, a diet with 1.30% yeast extract +4.80% squid viscera powder+1.90% fish soluble improves the immunity and antioxidant capacity of shrimp. Dietary 0.6% betaine reduces feed intake and promotes lipid utilization, and up-regulates the expression of immune-related genes of shrimp. This study showed that two dietary betaine supplementations on growth, feed utilization, lipid metabolism, and immune response of white shrimp (Litopenaeus vannamei) fed two levels of compound attractants, which provides reference value for white shrimp culture.

     

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