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田莹莹, 吴幸强, 姬燕培, 冯秀芳, 肖邦定. 一株红假单胞菌的分离及生理特性研究[J]. 水生生物学报, 2020, 44(2): 429-437. DOI: 10.7541/2020.052
引用本文: 田莹莹, 吴幸强, 姬燕培, 冯秀芳, 肖邦定. 一株红假单胞菌的分离及生理特性研究[J]. 水生生物学报, 2020, 44(2): 429-437. DOI: 10.7541/2020.052
TIAN Ying-Ying, WU Xin-Qiang, JI Yan-Pei, FENG Xiu-Fang, XIAO Bang-Ding. ISOLATION AND CHARACTERIZATION OF A RHODOPSEUDOMONAS SP. STRAIN[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(2): 429-437. DOI: 10.7541/2020.052
Citation: TIAN Ying-Ying, WU Xin-Qiang, JI Yan-Pei, FENG Xiu-Fang, XIAO Bang-Ding. ISOLATION AND CHARACTERIZATION OF A RHODOPSEUDOMONAS SP. STRAIN[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(2): 429-437. DOI: 10.7541/2020.052

一株红假单胞菌的分离及生理特性研究

ISOLATION AND CHARACTERIZATION OF A RHODOPSEUDOMONAS SP. STRAIN

  • 摘要: 光合细菌在水处理和水生态修复领域应用前景广阔, 对其生理特性研究具有重要价值。从武汉东湖分离得到一株不产氧光合细菌PUF1, 通过对菌落形态、细胞超微结构、特征吸收光谱以及系统发育等分析, 初步确定为红假单胞菌属(Rhodopseudomonas sp.)紫色非硫光合细菌。PUF1细胞呈直的或稍弯曲的杆状, 长3.05—10.06 μm, 直径0.32—0.68 μm, 具有片层膜结构。PUF1培养物呈深紫红色, 主要色素为细菌叶绿素a (Bchl. a)和类胡萝卜素。初始pH 6.0—8.0, 光照强度500—3000 lx, 稳定期细菌生物量无显著差异, 但培养液pH>8.0会显著抑制PUF1最大光量子产量(Fv/Fm)。试验进一步研究了细菌不同生长阶段粗蛋白含量、ATP酶(ATPase)活性及Fv/Fm的变化规律。结果表明, PUF1不同生长阶段其蛋白含量有所差异, 以稳定期为最高(>60%), 而ATPase活性随培养时间的延长而逐渐降低。此外, PUF1光合作用与其生长状态也有一定的关系, 具体表现为细菌生长周期内Fv/Fm变化规律适用单峰高斯模型, 且以对数期Fv/Fm为最高。研究结果可为光合细菌生理生化特性研究提供重要的参考依据。

     

    Abstract: Among the known subgroups of anoxygenic phototrophic bacteria, the Purple Nonsulfur Bacteria were widely investigated for their versatile metabolisms. This study isolated and identified an anoxygenic phototrophic bacterium of strain PUF1 from the East Lake, Wuhan, using the colony morphology, cell ultrathin structure, characteristic absorption spectra and phylogenetic analysis. The isolated strain was identified as Rhodopseudomonas sp. The bacterial cells are straight or slightly curved rod-shaped, 3.05—10.06 µm in length, 0.32—0.68 µm in diameter, with laminar membranes. The bacterial culture appears dark purple-red with major pigments being Bchl. a and carotenoids. With an initial pH of 6.0 to 8.0 and light intensity of 500 to 3000 lx, cell biomass at the stationary phase measured by the optical density (OD) showed no obvious difference, but pH higher than 8.0 in the liquid culture indeed decreased the maximal quantum yield of PS II (Fv/Fm). PUF1 had inconstant protein contents in its growth with the highest protein content of more than 60% related to the cellular dry weight in the stationary growth phase. The activity of ATPase continuously declined over the culture time. A plot of Fv/Fm fitted the single peak Gauss model with peak values in the log phase that showed certain relationships between the Fv/Fm and bacterial growing status. These findings provide important references for the study of physiological and biochemical characteristics of phototrophic bacteria.

     

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