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赵芳芳, 郎朗, 张左兵, 王兰. 镉胁迫对河南华溪蟹两种C型凝集素免疫应答的影响[J]. 水生生物学报, 2019, 43(2): 348-355. DOI: 10.7541/2019.043
引用本文: 赵芳芳, 郎朗, 张左兵, 王兰. 镉胁迫对河南华溪蟹两种C型凝集素免疫应答的影响[J]. 水生生物学报, 2019, 43(2): 348-355. DOI: 10.7541/2019.043
ZHAO Fang-Fang, LANG Lang, ZHANG Zuo-Bing, WANG Lan. EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(2): 348-355. DOI: 10.7541/2019.043
Citation: ZHAO Fang-Fang, LANG Lang, ZHANG Zuo-Bing, WANG Lan. EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(2): 348-355. DOI: 10.7541/2019.043

镉胁迫对河南华溪蟹两种C型凝集素免疫应答的影响

EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE

  • 摘要: C型凝集素是一类可以和糖类结合的蛋白质, 是先天性免疫系统中重要的模式识别受体。其中, 经典C型凝集素依赖Ca2+对糖类进行识别。Ca2+可作为细胞内第二信使, 参与多种信息传递。而重金属镉可导致细胞钙稳态失调, 干扰细胞内与Ca2+相关的信息传递。研究旨在探明镉胁迫对河南华溪蟹(Sinopotamon henanense) ShLec21和ShLec23两种C型凝集素免疫应答的影响。利用RACE方法, 克隆了ShLec21和ShLec23, 并进行了系统进化分析; 利用实时荧光定量PCR的方法, 研究了ShLec21和ShLec23的组织表达模式和镉联合嗜水气单胞菌(Aeromonas hydrophila)胁迫后肝胰腺和血淋巴中ShLec21和ShLec23表达模式。结果显示: ShLec21 cDNA全长863 bp, 编码152个氨基酸残基; ShLec23 cDNA全长681 bp, 编码164个氨基酸残基。ShLec21和ShLec23分别聚类为无脊椎动物的两个分支。ShLec21和ShLec23在血淋巴、鳃、肝胰腺、肠道、肌肉、卵巢和精巢中表达广泛, 但二者均主要在肝胰腺中表达。在胁迫条件下, 单独镉胁迫对肝胰腺和血淋巴中ShLec21和ShLec23表达量无显著影响; 在单独嗜水气单胞菌感染后, 肝胰腺中ShLec21和ShLec23表达量分别显著(P<0.05)与极显著(P<0.01)下调, 血淋巴中ShLec23表达量显著(P<0.05)下调; 而在镉胁迫后嗜水气单胞菌感染过程中,ShLec21和ShLec23表达量在肝胰腺和血淋巴中显著(P<0.05)或极显著(P<0.01)上调。研究结果表明, 河南华溪蟹ShLec21和ShLec23在响应嗜水气单胞菌感染过程中的表达, 在一定程度上能够被镉胁迫所上调。

     

    Abstract: C-type lectins are a type of proteins binding to carbohydrates and important pattern recognition receptors in the innate immune system. The classical C-type lectins recognize sugars in Ca2+-dependent manners. It is highly acknowledged that Ca2+ acts as a second messenger in the cell and participates in a variety of physiological and biochemical process. Heavy metal cadmium can lead to dysregulation of cellular calcium homeostasis and interfere with intracellular Ca2+-related information transmission. The aim of this study was to investigate the effects of cadmium stress on the immune responses of two types of lectins, ShLec21 and ShLec23, in the freshwater crab Sinopotamon henanense. The ShLec21 and ShLec23 cDNA were cloned by RACE method, and bioinformatic analysis was carried out. In addition to their constitutive expression in selected tissues, the stimulated expression of the two C-type lectins in hepatopancreas and hemolymph after the treatment of cadmium followed by Aeromonas hydrophila infection were detected. The results showed that ShLec21 cDNA was 863 bp in length that potentinally encoded 152 amino acid residues and ShLec23 cDNA was 681 bp in length that encoded 164 amino acid residues. ShLec21 and ShLec23 clustered into two branches of invertebrates. Both of ShLec21 and ShLec23 were widely expressed in hemolymph, gill, hepatopancreas, intestine, muscle, ovary and testis with highest level in hepatopancreas. Cadmium stress had no significant effect on the expression of ShLec21 and ShLec23 in hepatopancreas and hemolymph. Bacteria A. hydrophila infection significantly down-regulated the expression of ShLec21 (P<0.05) andShLec23 (P<0.01) in the hepatopancreas, and significantly (P<0.05) reduced the expression ofShLec23 in hemolymph. However, in the course of infection with A. hydrophila after cadmium stress, the expression levels of ShLec21 (P<0.05) andShLec23 (P<0.01) were significantly up-regulated in hepatopancreas and hemolymph. The results suggest that cadmium stress could upregulate the expression ofShLec21 and ShLec23 in response to A. hydrophila infection in a certain extent.

     

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