留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
李超, 张培育, 徐军, 张敏. 蜡质标本制作与保存对沉水植物稳定同位素的影响[J]. 水生生物学报, 2019, 43(3): 589-594. DOI: 10.7541/2019.071
引用本文: 李超, 张培育, 徐军, 张敏. 蜡质标本制作与保存对沉水植物稳定同位素的影响[J]. 水生生物学报, 2019, 43(3): 589-594. DOI: 10.7541/2019.071
LI Chao, ZHANG Pei-Yu, XU Jun, ZHANG Min. EFFECTS OF PREPARATION AND PRESERVATION OF HERBARIUM SPECIMENS ON STABLE ISOTOPES OF SUBMERGED MACROPHYTES[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 589-594. DOI: 10.7541/2019.071
Citation: LI Chao, ZHANG Pei-Yu, XU Jun, ZHANG Min. EFFECTS OF PREPARATION AND PRESERVATION OF HERBARIUM SPECIMENS ON STABLE ISOTOPES OF SUBMERGED MACROPHYTES[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 589-594. DOI: 10.7541/2019.071

蜡质标本制作与保存对沉水植物稳定同位素的影响

EFFECTS OF PREPARATION AND PRESERVATION OF HERBARIUM SPECIMENS ON STABLE ISOTOPES OF SUBMERGED MACROPHYTES

  • 摘要: 实验通过模拟传统方法制作和保存蜡质标本, 旨在探究制作和保存的不同阶段对3种沉水植物碳、氮稳定同位素值的影响。结果表明, 制作和保存标本的过程均会对沉水植物的碳、氮稳定同位素值产生影响。植物标本压制的过程显著降低了沉水植物平均碳稳定同位素值, 其中马来眼子菜下降了1.78‰, 穗花狐尾藻下降了0.98‰, 金鱼藻下降了0.72‰。在压制过程中氮稳定同位素值只有金鱼藻显著下降了1.1‰。沉水植物标本制作的浸泡过程使穗花狐尾藻和马来眼子菜的碳稳定同位素值分别出现了1.00‰和0.52‰的上升, 金鱼藻的碳稳定同位素值增长小于0.08‰, 氮稳定同位素值没有显著变化。沉水植物对标本制作和保存的过程响应存在种间差异。在制作和保存过程中对植物碳稳定同位素产生的影响大于氮稳定同位素值。因此建议, 如果所研究对象的稳定同位素生态学差异小于3‰, 蜡质标本的样品需要进行实验校准, 不然会对结果产生影响。

     

    Abstract: Carbon and nitrogen stable isotopes of herbarium specimens have been used by ecologists to study long-history pollution and climate change. However, whether the preparation and preservation procedure impact isotopic signatures of herbarium specimens remain unclear. We tested the effect of herbarium on three submerged macrophyte species, and found the variations by both preparation and preservation processes. The press procedure significantly reduced carbon stable isotopes of submerged macrophytes for P. malaianus by 1.78‰, M. spicatum by 0.98‰ and C. demersum by 0.72‰. The press procedure reduced C. demersum nitrogen stable isotopes by 1.1‰. The soak procedure did not affect nitrogen stable isotopes of submerged macrophytes, but increased carbon stable isotopes of M. spicatum and P. malaianus by 1.00‰ and 0.52‰, separately. Carbon stable isotopes of C. demersum increased less than 0.08‰. Preparing procedures had larger effect on carbon isotope than nitrogen isotope. These influences were highly variable between species and times. The results suggest that if the interested stable isotopic variations are within 3‰, herbarium specimens are not suitable samples for the relevant studies without the experimental corrections.

     

/

返回文章
返回