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赵玉蓉, 付莹, 王红权. α-酮戊二酸对氨氮胁迫下草鱼鳃Na+/K+-ATP酶活性及血液生化指标的影响[J]. 水生生物学报, 2018, 42(5): 996-1002. DOI: 10.7541/2018.122
引用本文: 赵玉蓉, 付莹, 王红权. α-酮戊二酸对氨氮胁迫下草鱼鳃Na+/K+-ATP酶活性及血液生化指标的影响[J]. 水生生物学报, 2018, 42(5): 996-1002. DOI: 10.7541/2018.122
ZHAO Yu-Rong, FU Ying, WANG Hong-Quan. EFFECTS OF α-KETOGLUTARATE ON GILL NA+/K+-ATPASE ACTIVITY AND BLOOD BIOCHEMICAL PARAMETERS OF GRASS CARP EXPOSURE TO AMMONIA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 996-1002. DOI: 10.7541/2018.122
Citation: ZHAO Yu-Rong, FU Ying, WANG Hong-Quan. EFFECTS OF α-KETOGLUTARATE ON GILL NA+/K+-ATPASE ACTIVITY AND BLOOD BIOCHEMICAL PARAMETERS OF GRASS CARP EXPOSURE TO AMMONIA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 996-1002. DOI: 10.7541/2018.122

α-酮戊二酸对氨氮胁迫下草鱼鳃Na+/K+-ATP酶活性及血液生化指标的影响

EFFECTS OF α-KETOGLUTARATE ON GILL NA+/K+-ATPASE ACTIVITY AND BLOOD BIOCHEMICAL PARAMETERS OF GRASS CARP EXPOSURE TO AMMONIA

  • 摘要: 试验旨在研究饲粮添加α-酮戊二酸(α-ketoglutarate, α-KG)对氨氮胁迫下草鱼(Ctenopharyngodon idellus)鳃Na+/K+-ATP酶活性及血液生化指标的影响。选取初始体重为(24.79±0.11) g的健康草鱼, 随机分为3个处理组(对照组, 养于曝气后氨氮浓度为1.51 mg/L的自来水中并饲喂基础饲粮; 氨氮组, 养于氨氮浓度为18.37 mg/L的水中并饲喂基础饲粮; α-KG组, 养于氨氮浓度为18.37 mg/L的水中并饲喂添加0.75% α-KG的饲粮)。每处理组设3个重复, 每个重复30尾鱼, 养殖试验为期42d, 分别于第1、第14、第28和第42天采样。结果表明:在饲料中添加α-KG能够有效缓解氨氮胁迫导致的草鱼血浆氨含量(1d)、谷草转氨酶(Aspartate transaminase, AST)活性(14d)、碱性磷酸酶(Alkaline phosphatase, ALP)活性(28d)的显著升高和鳃Na+/K+-ATP酶活性(28d)、血浆谷丙转氨酶(Alanine aminotransaminase, ALT)(28d和42d)活性、血浆尿素(UREA)含量(28d)的显著降低, 显著增加氨氮胁迫下草鱼血浆球蛋白(Globulin, GLB)含量(28d)。即饲粮α-KG的适量添加能够有效缓解草鱼氨氮胁迫所致的血氨含量升高, 维持氨氮胁迫下草鱼鳃Na+/K+-ATP酶、血浆谷丙转氨酶、谷草转氨酶、碱性磷酸酶的活性和血浆球蛋白、尿素含量的稳定, 从而有利于草鱼缓解氨氮胁迫。

     

    Abstract: A 42-day feeding trial was conducted to investigate the effects of α-ketoglutarate (α-KG) on gill Na+/K+-ATPase activity and blood biochemical parameters of grass carp, Ctenopharyngodon idellus, exposure to ammonia. A total of 270 individuals initial body weight: (24.79±0.11) g were randomly assigned to 9 tanks, and these tanks were divided into three groups (Ⅰ: treated with 1.51 mg/L of aerated water and basal diet; Ⅱ: treated with 18.37 mg/L of ammonia solution and basal diet; Ⅲ: treated with 18.37 mg/L of ammonia solution and 0.75% α-KG added diet), with three replicates per each group (n=30). Experimental fish were sampled on 1st, 14th, 28th, and 42 days, respectively. The results showed that: (1) diet α-KG could effectively alleviate a significant increase in the content of plasma ammonia on the 1st day, the activity of plasma aspartate transaminase on 14th day and plasma alkaline phosphatase on 28th day, which were caused as being exposure to ammonia; diet α-KG could effectively alleviate a significant decrease in the activity of gill Na+/K+-ATPase on 28th day, plasma alanine aminotransferase on 28th and 42th days, and the content of plasma urea on 28th day because of being exposure to ammonia. Diet α-KG could significantly increase plasma globulin content on 28th day exposure to ammonia. In conclusion, dietary α-KG could alleviate the increase of plasma ammonia content exposure to ammonia, and maintain the stabilization in the activity of gill Na+/K+-ATPase, plasma alanine transaminase (ALT), plasma aspartate aminotransferase (AST), plasma alkaline phosphatase (ALP), and the content of GLB, as well as urea in grass carp exposure to ammonia.

     

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