利用草鱼CIK细胞和MTT法测定镉和铬毒性试验的优化
OPTIMUZATION OF MTT ASSAY FOR THE DETEMINATION OF CYTOTOXICITY OF Cd2+ AND Cr6+ IN KINDY CELL LINE OF GRASS CARP
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摘要: 本文利用主要养殖鱼类草鱼的肾脏细胞系CIK为靶细胞,从MTT法的细胞接种浓度、MTT 浓度及温育时间等几个重要参数,研究了对CIK细胞应用MTT法进行化合物毒性测定的条件,并根据最适条件,测定了氯化镉(CdCl2) 和重铬酸钾(K2Cr2O6) 对草鱼CIK细胞的毒性,建立了一种利用鱼类细胞系(草鱼CIK细胞) 进行化合物毒性测定的方法。由于每种细胞系各具有不同的生物学特性及对有毒物质不同的敏感性,因此该测定方法的建立,在水产养殖环境毒理的领域中,将具有一定的应用价值。
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Keywords:
- MTT /
- CIKof grass carp /
- Cd2+ /
- Cr6+ /
- Cytotoxicity
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[1] Olabarrieta I, Azou B L, Yuric S,et al. In vitro effects of cadmium on two different animal cell models[J]. Toxicology in Vitro, 2001,15:511-517
[2] Bian X Y, Chen J, A comparison of five formazan dissolution in MTT Assay[J]. Chinese Journal of Clinical Laboratory Science. 1998,16(1):34-35[边兴艳,陈洁.MTT比色法中五种甲瓒溶解液的比较[J].临床检验杂志,1998,16(1):34-35]
[3] Chen J P, Xu L H, FU W Y,et al. Bax expression of fat nephrocyte NRK induced by microcystin LR[J].Acta Hydrobioligica Sinica,2004,28(4):450-451[ 陈加平,徐立红,傅文宇,等. 微囊藻毒素LR 诱导大鼠肾NRK细胞Bax 蛋白表达[J]. 水生生物学报,2004,28(4):450-451]
[4] Chen J P, FU W Y, Wen D P,et al. Damage of microcystin LR on rat lymphocytes DNA[J]. Acta Hydrobioligica Sinica, 2004,28(6):677-678[陈加平,傅文宇,翁登坡,等. 微囊藻毒素LR 对大鼠淋巴细胞DNA 的损伤作用[J]. 水生生物学报,2004,28(6):677-678]
[5] Sauvant M. P, Peptin D, Bohatier J, et al. Toxicity assessment of 16 inorganic environmental pollutants by six bioassays[J]. Ecotoxicology and Environmental Safety, 1997,37:131-140
[6] Ma H Z,Sheng H Z, Qin A P,et al. Comparative study on toxicities of potassium dichromate and chromium chloride [J]. Carcinogenesis, Teratogenesis and Mitagenesis, 2003,15(3):171-173[ 马华智,盛和章,秦爱平,等. 氯化铬和重铬酸钾毒性的比较研究[J]. 癌变·畸变·突变,2003,15(3):171-173]
[7] Maracine M and Segner H. Cytotoxicity of metals in isolated fish cells: Importance of the celluar glutathione ststus[J]. Comparative Biochemistry and Physiology. Part A, 1998,120:83-88
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