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陈靖雯, 郭道远, 赵冰, 曹小娟, 高坚. 饲料β-葡聚糖和灭活乳酸菌的添加对泥鳅幼鱼生长性能、肠脂肪酸组成及免疫性能的影响[J]. 水生生物学报, 2019, 43(1): 52-59. DOI: 10.7541/2019.007
引用本文: 陈靖雯, 郭道远, 赵冰, 曹小娟, 高坚. 饲料β-葡聚糖和灭活乳酸菌的添加对泥鳅幼鱼生长性能、肠脂肪酸组成及免疫性能的影响[J]. 水生生物学报, 2019, 43(1): 52-59. DOI: 10.7541/2019.007
CHEN Jing-Wen, GUO Dao-Yuan, ZHAO Bing, CAO Xiao-Juan, GAO Jian. EFFECTS OF DIETARY SUPPLEMENTATION OF β-GLUCAN AND HEAT-KILLED LACTOBACILLUS PLANTARUM ON GROWTH PERFORMANCE, INTESTINAL FATTY ACID COMPOSITION AND IMMUNE RESPONSE OF DOJO LOACH MISGURNUS ANGUILLICAUDATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(1): 52-59. DOI: 10.7541/2019.007
Citation: CHEN Jing-Wen, GUO Dao-Yuan, ZHAO Bing, CAO Xiao-Juan, GAO Jian. EFFECTS OF DIETARY SUPPLEMENTATION OF β-GLUCAN AND HEAT-KILLED LACTOBACILLUS PLANTARUM ON GROWTH PERFORMANCE, INTESTINAL FATTY ACID COMPOSITION AND IMMUNE RESPONSE OF DOJO LOACH MISGURNUS ANGUILLICAUDATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(1): 52-59. DOI: 10.7541/2019.007

饲料β-葡聚糖和灭活乳酸菌的添加对泥鳅幼鱼生长性能、肠脂肪酸组成及免疫性能的影响

EFFECTS OF DIETARY SUPPLEMENTATION OF β-GLUCAN AND HEAT-KILLED LACTOBACILLUS PLANTARUM ON GROWTH PERFORMANCE, INTESTINAL FATTY ACID COMPOSITION AND IMMUNE RESPONSE OF DOJO LOACH MISGURNUS ANGUILLICAUDATUS

  • 摘要: 为研究饲料中添加β-葡聚糖(BG)和热灭活乳酸菌(HK-LP)对泥鳅(Misgurnus anguillicaudatus)幼鱼生长性能、肠道脂肪酸组成及免疫功能的影响,采用2×3双因素设计,设2个BG (0和1%)和3个HK-LP (0.025%、0.05%和0.1%)水平,选择初始均重为(0.17±0.01) g的泥鳅幼鱼,分别投喂6种配合饲料,每种饲料3个重复,饲养期为80d。结果显示,在饲料中分别添加BG和HK-LP显著提高了泥鳅幼鱼的终末体重、增重率、特定成长率,显著降低了饲料系数。然而,2种添加剂的相互作用没有对这些生长指标产生影响。添加BG显著降低了肠道脂肪酸中C16:1n-7和C18:2n-6的比率,同时随着HK-LP添加显著降低了C22:1n-11的比率。BG、HK-LP及两因素的相互作用均显著影响了体表黏液中碱性磷酸酶(AKP)活性,随着BG及HK-LP添加量的增加,溶菌酶(LZM)活性出现升高趋势。添加1%BG和0.05%HK-LP饲料,显著上调了热休克蛋白HSP70和HSP90α的表达水平,同时在BG未添加组,随着HK-LP添加的增加,上调了TLR1的表达水平。综上所述,饲料BG和HK-LP添加可以改善泥鳅幼鱼生长性能,其中BG 1%和HK-LP 0.1%的实验组对泥鳅幼鱼生长的促进作用最为显著。

     

    Abstract: A 80-day feeding trial was conducted to assess the interactive effects of β-glucan (BG) and Heat-killed Lactobacillus plantarum (HK-LP) on growth performance, intestinal fatty acid composition, and immune function of juvenile loach Misgurnus anguillicaudatus. Fish average initial weight of (0.17±0.01) g were fed with six experimental formulated diets containing two levels of BG (0 and 1%) with three levels of HK-LP (0.025%, 0.05%, and 0.1%) in triplicate. Results showed that supplementation of BG and HK-LP increased the body weight gain, specific growth rate, while significantly reduced the feed conversion ratio of fish. However, interaction between BG and HK-LP showed no significant effect on those parameters. Supplementation of BG remarkably decreased the percentage of C16鲶1n-7 and C18鲶2n-6 in intestinal fatty acid composition. Meanwhile, the percentage of C22 鲶1n-11 was decreased by dietary HK-LP. Dietary BG, HK-LP and two-factor interactions significantly influenced the activities of alkaline phosphatase (AKP) from skin mucus. Increase of dietary BG and HK-LP levels enhanced the activities of lysozyme. Supplementation of dietary 1% BG and 0.05% HK-LP significantly increased the expression levels of Hsp70 and Hsp90α. Therefore, the addition of BG and HK-LP in the diets could improve the growth performance of juvenile loach, and in the experiment, the optimum level of BG and HK-LP in the loach diet was 1% and 0.1%, respectively.

     

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