留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
廖德杰, 曹善茂, 童金苟, 周颖, 俞小牧, 刘阳, 王潇. 扇贝核糖体ITS1和5S rDNA序列的遗传变异及亲缘关系分析[J]. 水生生物学报, 2018, 42(3): 494-502. DOI: 10.7541/2018.062
引用本文: 廖德杰, 曹善茂, 童金苟, 周颖, 俞小牧, 刘阳, 王潇. 扇贝核糖体ITS1和5S rDNA序列的遗传变异及亲缘关系分析[J]. 水生生物学报, 2018, 42(3): 494-502. DOI: 10.7541/2018.062
LIAO De-Jie, CAO Shan-Mao, TONG Jin-Gou, ZHOU Ying, YU Xiao-Mu, LIU Yang, WANG Xiao. GENETIC VARIATION AND RELATIONSHIP ANALYSIS OF SCALLOPS BASED ON THE SEQUENCES OF ITS1 AND 5S rDNA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 494-502. DOI: 10.7541/2018.062
Citation: LIAO De-Jie, CAO Shan-Mao, TONG Jin-Gou, ZHOU Ying, YU Xiao-Mu, LIU Yang, WANG Xiao. GENETIC VARIATION AND RELATIONSHIP ANALYSIS OF SCALLOPS BASED ON THE SEQUENCES OF ITS1 AND 5S rDNA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(3): 494-502. DOI: 10.7541/2018.062

扇贝核糖体ITS1和5S rDNA序列的遗传变异及亲缘关系分析

GENETIC VARIATION AND RELATIONSHIP ANALYSIS OF SCALLOPS BASED ON THE SEQUENCES OF ITS1 AND 5S rDNA

  • 摘要: 为探讨引进种岩扇贝(Crassadoma gigantea)及虾夷扇贝(Patinopecten yessoensis)、栉孔扇贝(Chlamys farreri)和海湾扇贝(Argopecten irradians)的遗传变异及其之间的亲缘关系, 研究对4种扇贝的核糖体ITS1序列及5S rDNA序列进行了测定, 获得了4种扇贝的ITS1和5S rDNA全序列以及18S rDNA的部分序列, 并进行了序列特征、遗传多样性、遗传距离及系统发育等相关分析。结果显示, 基于ITS1序列的单倍型数、核苷酸变异位点、单倍型多样性指数、核苷酸多样性指数及平均核苷酸差异数分别在2—6、1—12、0.425—0.800、0.00081—0.00486和0.366—2.442; 基于5S 序列的相应参数分别在2—5、1—14、0.533—0.841、0.00108—0.01058和0.533—5.726。4种扇贝均表现出较低的多样性水平(π<0.01)。此外, 4种扇贝基于2种方法计算的种间遗传距离分别在0.040—0.121和0.042—0.413, 均表现为岩扇贝与虾夷扇贝的遗传距离最近, 而岩扇贝与海湾扇贝遗传距离最远。并且系统进化树分析显示岩扇贝与虾夷扇贝独自聚为一支, 说明引进物种岩扇贝与虾夷扇贝的亲缘关系较近。研究结果将为岩扇贝的种质资源保护以及今后的遗传育种研究工作提供参考信息。

     

    Abstract: Rock scallop Crassadoma gigantea was introduced to China in 2012, and was successfully bred in 2015. It is in the stage of popularizing culture now. To understand the genetic relationships and the genetic variations among C. gigantea, Argopecten irradians, Chlamys farreri, and Patinopecten yessoensis, the whole sequences of ITS1 and 5S rDNA were sequenced after the amplification by PCR. The sequence characterization, genetic diversity, genetic distance, and phylogeny were determined by using a suite of biology softwares. Based on the sequences of ITS1, the numbers of haplotype and polymorphic sites, haplotype diversities, nucleotide diversities, and average nucleotide differences were varied from 2 to 6, 1 to 12, 0.425 to 0.800, 0.00081 to 0.00486 and 0.366 to 2.44, respectively. In addition, based on the sequences of 5S rDNA, their same genetic variation parameters varied from 2 to 5, 1 to 14, 0.533 to 0.841, 0.00108 to 0.1058 and 0.533 to 5.726, respectively. The result showed that all these four species had a low diversity level (π<0.01). Based on the sequences ofITS1 and 5S rDNA, the genetic distance were varied from 0.040 to 0.124, and 0.043 to 0.457, respectively, which demonstrated that C. gigantea appeared to be closely-related to P. yessoensis. Meanwhile, phylogeny trees of the genetic distances confirmed the genetic relatedness between C. gigantea and P. yessoensis. This work would provide basic information for future studies on germplasm resources management, as well as on the genetics and breeding strategies of C. gigantea.

     

/

返回文章
返回