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张红波, 董聪聪, 杨燕君, 付君珂, 刘黎, 贺新宇, 施军琼, 吴忠兴. 基于叶绿素荧光探讨链霉素对念珠藻生长及光合毒性效应[J]. 水生生物学报, 2019, 43(3): 664-669. DOI: 10.7541/2019.079
引用本文: 张红波, 董聪聪, 杨燕君, 付君珂, 刘黎, 贺新宇, 施军琼, 吴忠兴. 基于叶绿素荧光探讨链霉素对念珠藻生长及光合毒性效应[J]. 水生生物学报, 2019, 43(3): 664-669. DOI: 10.7541/2019.079
ZHANG Hong-Bo, DONG Cong-Cong, YANG Yan-Jun, FU Jun-Ke, LIU Li, HE Xin-Yu, SHI Jun-Qiong, WU Zhong-Xing. THE TOXIC EFFECT OF STREPTOMYCIN ON THE GROWTH AND PHOTOSYNTHESIS OF NOSTOC USING THE CHLOROPHYLL FLUORESCENCE ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 664-669. DOI: 10.7541/2019.079
Citation: ZHANG Hong-Bo, DONG Cong-Cong, YANG Yan-Jun, FU Jun-Ke, LIU Li, HE Xin-Yu, SHI Jun-Qiong, WU Zhong-Xing. THE TOXIC EFFECT OF STREPTOMYCIN ON THE GROWTH AND PHOTOSYNTHESIS OF NOSTOC USING THE CHLOROPHYLL FLUORESCENCE ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(3): 664-669. DOI: 10.7541/2019.079

基于叶绿素荧光探讨链霉素对念珠藻生长及光合毒性效应

THE TOXIC EFFECT OF STREPTOMYCIN ON THE GROWTH AND PHOTOSYNTHESIS OF NOSTOC USING THE CHLOROPHYLL FLUORESCENCE ANALYSIS

  • 摘要: 为了探究不同浓度链霉素对念珠藻生长和光合的毒性效应, 研究对不同浓度(0、0.05、0.1、0.2、0.5和1.0 mg/L)链霉素处理下念珠藻的生长、叶绿素荧光进行了测定。结果发现, 培养至96h, 链霉素对念珠藻的EC50(96h-EC50)为(0.13±0.037) mg/L。与对照组相比, 各链霉素浓度处理下叶绿素a的含量显著降低。最大光化学效率(φP0)、可变荧光(Fv)、PSⅡ的潜在活性(Fv/F0)、单位面积上有活性的反应中心(RC/CS0)、以吸收光能为基础的性能指数(PIabs)及反应中心吸收的光能用于电子传递的量子产额(φE0)均随着链霉素浓度的升高而降低。然而, 单位叶绿素最大荧光(Fm/Chl. a)、每个有活性的反应中心吸收的光能(ABS/RC)和热耗散(DI0/RC)则随着链霉素浓度的升高而增加。当链霉素浓度大于0.1 mg/L时, 叶绿素荧光动力学曲线出现K点的出现。结果表明链霉素可能通过抑制光合系统Ⅱ(PSⅡ)的电子传递和减少有活性的反应中心来影响念珠藻的生长, 也暗示了高浓度链霉素对藻类的生长具有抑制作用。

     

    Abstract: To explore the toxic effects of streptomycin, the growth and chlorophyll fluorescence were measured in Nostoc treated with different concentrations (0, 0.05, 0.10, 0.20, 0.50 and 1 mg/L) of streptomycin. The results indicated that the EC50 of streptomycin to Nostoc (96h-EC50) was (0.13±0.037) mg/L at the 96h. Streptomycin significantly decreased the content of chlorophyll a at all concentrations. In a concentration-dependent pattern, streptomycin significantly reduced the maximum photochemical efficiency (φP0), variable fluorescence (Fv), potential activity of PSⅡ (Fv/F0), active reaction center per unit area (RC/CS0), the performance index based on absorbed light energy (PIabs) and the quantum energy yield of the light energy absorbed by the reaction center for electron transfer (φE0). However, Streptomycin significantly increased the maximum fluorescence relative to chlorophyll a (Fm/Chl. a), the light energy absorbed by each active reaction center (ABS/RC) and heat dissipation (DI0/RC) in a concentration-dependent pattern. A K-point at chlorophyll a fluorescence transient curve was found when the concentration is greater than 0.1 mg/L. These results suggest that streptomycin might affect the growth of Nostoc by inhibiting the electron transport of photosynthetic system Ⅱ (PSⅡ) and reducing active reaction centers, and that high concentrations of streptomycin showed an inhibitory effect on the growth of algae.

     

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