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黄康, 梁旭方, 何珊, 李姣, 汤树林, 张真. 异亮氨酸对鳜mTOR信号通路及氮代谢影响[J]. 水生生物学报, 2018, 42(5): 879-886. DOI: 10.7541/2018.108
引用本文: 黄康, 梁旭方, 何珊, 李姣, 汤树林, 张真. 异亮氨酸对鳜mTOR信号通路及氮代谢影响[J]. 水生生物学报, 2018, 42(5): 879-886. DOI: 10.7541/2018.108
HUANG Kang, LIANG Xu-Fang, HE Shan, LI Jiao, TANG Shu-Lin, ZHANG Zhen. EFFECT OF ISOLEUCINE ON mTOR SIGNALING PATHWAY AND NITROGEN METABOLISM OF CHINESE PERCH (SINIPERCA CHUATSI)[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 879-886. DOI: 10.7541/2018.108
Citation: HUANG Kang, LIANG Xu-Fang, HE Shan, LI Jiao, TANG Shu-Lin, ZHANG Zhen. EFFECT OF ISOLEUCINE ON mTOR SIGNALING PATHWAY AND NITROGEN METABOLISM OF CHINESE PERCH (SINIPERCA CHUATSI)[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 879-886. DOI: 10.7541/2018.108

异亮氨酸对鳜mTOR信号通路及氮代谢影响

EFFECT OF ISOLEUCINE ON mTOR SIGNALING PATHWAY AND NITROGEN METABOLISM OF CHINESE PERCH (SINIPERCA CHUATSI)

  • 摘要: 通过脑室注射异亮氨酸, 探究短期内异亮氨酸对鳜(Siniperca chuatsi)雷帕霉素靶蛋白(Mammalian target of rapamycin, mTOR)信号通路及氮代谢影响。结果显示: 脑室注射异亮氨酸后, (1)促进鳜氨氮排泄; (2)谷氨酸脱氢酶基因(Glutamate dehydrogenase, GDH)、谷草转氨酶基因(Glutamic oxaloacetic transaminase, GOT)和腺苷酸脱氨酶基因(Adenosine monophosphate deaminase, AMPD)氮代谢基因相对表达量显著性上调(P<0.05); (3)鳜血糖含量在0.5h显著性降低(P<0.05); (4)激活了鳜肝脏mTOR信号通路, 促使下游分子核糖体蛋白S6磷酸化(P<0.05)。结果表明: 异亮氨酸能够激活鳜肝脏mTOR信号通路, 介导氨基酸代谢, 提高鳜氮代谢基因的转录水平, 促使氨氮排泄增多。

     

    Abstract: In the present study, the effects of isoleucine on the mammalian target of rapamycin signaling (mTOR) pathway and nitrogen metabolism were investigated through Intraventricular (ICV) administration in Chinese perch (Siniperca chuatsi). In one hand, the ammonia excretion significantly increased, and mRNA levels of nitrogen metabolism genes involving glutamate dehydrogenase (GDH), glutamic oxaloacetic transaminase (GOT), and adenosine monophosphate deaminase (AMPD) remarkably increased after ICV injection of isoleucine as well (P<0.05). On the other hand, the results showed that blood glucose levels markedly decreased at 0.5h post-injection. The expression of liver ribosomal protein S6 was also notably enhanced, demonstrating that mTOR signaling pathway was activated. The results also indicated that isoleucine could activate the mTOR signaling pathway, mediate amino acid metabolism, and enhance ammonia-N excretion via nitrogen metabolism genes in Chinese perch.

     

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