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肖扬波, 曹申平, 敖青, 黄康, 莫郁坚, 张欣然, 郑欣怡, 童晓念, 毛庄文, 樊均德, 刘臻, 唐建洲. 低鱼粉饲料中黑水虻幼虫粉替代鱼粉对合方鲫生长性能、消化能力、血浆生化指标及相关基因表达的影响[J]. 水生生物学报, 2023, 47(9): 1363-1373. doi: 10.7541/2023.2022.0476
引用本文: 肖扬波, 曹申平, 敖青, 黄康, 莫郁坚, 张欣然, 郑欣怡, 童晓念, 毛庄文, 樊均德, 刘臻, 唐建洲. 低鱼粉饲料中黑水虻幼虫粉替代鱼粉对合方鲫生长性能、消化能力、血浆生化指标及相关基因表达的影响[J]. 水生生物学报, 2023, 47(9): 1363-1373. doi: 10.7541/2023.2022.0476
XIAO Yang-Bo, CAO Shen-Ping, AO Qing, HUANG Kang, MO Yu-Jian, ZHANG Xin-Ran, ZHENG Xin-Yi, TONG Xiao-Nian, MAO Zhuang-Wen, FAN Jun-De, LIU Zhen, TANG Jian-Zhou. REPLACING FISH MEAL WITH HERMETIA ILLUCENS LARVAE MEAL ON GROWTH PERFORMANCE, DIGESTIVE CAPACITY, PLASMA BIOCHEMICAL INDEXES AND RELATED GENES EXPRESSION IN HEFANG CRUCIAN CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1363-1373. doi: 10.7541/2023.2022.0476
Citation: XIAO Yang-Bo, CAO Shen-Ping, AO Qing, HUANG Kang, MO Yu-Jian, ZHANG Xin-Ran, ZHENG Xin-Yi, TONG Xiao-Nian, MAO Zhuang-Wen, FAN Jun-De, LIU Zhen, TANG Jian-Zhou. REPLACING FISH MEAL WITH HERMETIA ILLUCENS LARVAE MEAL ON GROWTH PERFORMANCE, DIGESTIVE CAPACITY, PLASMA BIOCHEMICAL INDEXES AND RELATED GENES EXPRESSION IN HEFANG CRUCIAN CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1363-1373. doi: 10.7541/2023.2022.0476

低鱼粉饲料中黑水虻幼虫粉替代鱼粉对合方鲫生长性能、消化能力、血浆生化指标及相关基因表达的影响

REPLACING FISH MEAL WITH HERMETIA ILLUCENS LARVAE MEAL ON GROWTH PERFORMANCE, DIGESTIVE CAPACITY, PLASMA BIOCHEMICAL INDEXES AND RELATED GENES EXPRESSION IN HEFANG CRUCIAN CARP

  • 摘要: 实验以合方鲫2号幼鱼(31.50±0.50) g为实验对象, 研究黑水虻幼虫粉(HM)替代饲料鱼粉(0、20%、40%、60%和80%)对合方鲫生长性能、饲料利用、消化能力、血浆生化指标及相关基因表达的影响。实验设计5组等氮(30%)等能(18.50 MJ/kg)等脂(6%)饲料, 每组3次重复, 每个重复25尾鱼, 养殖时间74d。结果表明: 增重率和特定生长率随饲料HM含量的升高呈现先升高后降低趋势, 且在替代水平为20%时最大; 饲料系数呈先降低后升高的趋势, 20%替代组显著小于60%和80%替代组(P<0.05)。肥满度和脏体比均呈现先升高后降低趋势, 且均在40%替代组最大; 80%替代组肝体比显著高于0、20%和60%替代组(P<0.05), 但与40%替代组无显著差异。全鱼水分和灰分无显著变化; 20%和60%替代组粗蛋白显著低于0组(P<0.05); 粗脂肪呈先升高后降低的变化趋势, 在20%替代组达到最大值。血浆葡萄糖、总胆固醇含量及谷丙转氨酶、谷草转氨酶活性无显著差异; 血氨含量呈上升趋势, 80%替代组显著高于0组; 20%替代组血浆总氨基酸含量显著高于其他各处理组(P<0.05); 60%和80%替代组血浆丙二醛含量显著低于20%替代组(P<0.05)。20%替代组肠道中lat2pept1cdx2基因的表达量均高于其他各处理组, asct2基因表达水平则在替代水平为40%时最大; 肝脏中torigf1基因表达量均在40%替代组最大, ghghr基因表达水平则均在替代水平为60%时最高。黑水虻幼虫粉替代饲料中鱼粉的0—40%不影响合方鲫的生长, 且在20%替代水平时可获得最大生长速率。

     

    Abstract: To investigate the effects of dietary replacement fishmeal protein with Hermetia illucens larvae meal (HM) on growth performance, digestive capacity, plasma biochemical indexes and related genes expression in Hefang crucian carp (Carassis auratus), five isonitrogenous (30%), isolipidic (6%) and isoenergetic (18.50 MJ/kg) diets were formulated to contain graded levels of fishmeal protein replacement by HM (0, 20%, 40%, 60% and 80% respectively) for a 74-day feeding trial. Each treatment was randomly assigned to triplicate groups of 25 fish initial body weight of (31.50±0.50) g per aquarium. Fish were fed twice daily to apparent satiation. Results showed that the weight gain rate (WGR) and specific growth rate (SGR) first increased and then decreased with the increased proportion of HM in diets, with a maximum in the 20% substitution group. Feed conversion ratio (FCR) showed an initial decrease followed by an increase, and the 20% substitution group was significantly less than 60% and 80% substitution groups (P<0.05). With the increased proportion of HM in diets, condition factor (CF) and viscerosomatic index (VSI) first increased and then decreased, with a maximum in the 40% substitution group. The hepatopancreas somatic index (HSI) of 80% substitution group was significantly higher than those in 0, 20% and 60% substitution groups (P<0.05), but there was no significant difference between 40% and 80% substitution group. The moisture and ash of fish body expressed no significant difference. However, crude protein of body in 20% and 60% substitution group was significantly lower than that in 0 group (P<0.05). Crude lipid of body showed an initial increase followed by a decrease, and the highest value was seen in 20% substitution group. There was no significant difference in plasma glucose concentration, total cholesterol concentration, alanine aminotransferase and aspartate aminotransferase activity, but blood ammonia content showed an upward trend, the blood ammonia of 80% substitution group was significantly higher than that of 0 substitution group. Total plasma amino acid in the 20% substitution group was significantly higher than those in the other treatments (P<0.05). Plasma malondialdehyde was significantly lower in 60% and 80% substitution groups than that in 20% substitution group (P<0.05). The expression of lat2, pept1 and cdx2 genes in the intestine reached to the highest level in 20% substitution group, while asct2 gene showed the highest expression in 40% substitution group. The expression of tor and igf1 genes in liver was the highest in 40% substitution group, while the highest expression of gh and ghr genes were seen in 60% substitution group. These results suggested that 0—40% of fish meal in the diet of HCC could be substituted by HM, and 20% was the optimal optimum proportion.

     

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