Abstract: In order to basically figure out the metabolism and demand on the fatty acids of rice field eel, Monopterusalbus Zuiew, and provide a frame of reference on selective using different lipid sources for rice diets production of fieldeel, six isonitrogenous (44% crude protein) and isoenergetic (19.4 MJ/kg) experimental diets were formulated by usingsix different lipid sources: fish oil (FO), soybean oil (SO), and linseed oil (LO), ARA + peanut oil (APO), peanut oil (PO)and pork lard (PL). Female rice field eels (initial weight of 15.5 g) were fed with these diets for 10 weeks. The fats andfatty acid compositions in the muscle liver and ovary of these eels were analyzed by means of chloroform- methanol andgas chromatography (GC) and the growth performance were evaluated with the parameter of specific growth rate (SGR).The results showed that there were effects of different lipid sources on the growth performance of rice field eels. TheSGR of rice field eels fed with PO without n-3 polyunsaturated fatty acids (PUFAs) was significantly lower than thosefed with SO and FO (P0.05); the specific growth rate (SGR) of rice field eels fed with SO was significantly higher thanthose fed with LO, APO, PO and PL, except FO (P0.05). There were positive linear correlation between the contentboth 18:3n-3 and 18:2n-6 in the various tissues of rice field eels and those in experimental diets. When the experimentdiets were lack of eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), there were positivelogarithmic correlation between the content of EPA, DHA in ovaries and the content of 18:3n-3 in diets; while the experimentaldiets were lack of Arachidonic acid(ARA, 20:4n-6), there were positive linear correlation between the contentof ARA in muscle, liver and ovary and the content of 18:2n-6 in diets. The content of EPA and DHA in the varioustissues of the rice field eels fed with diet (FO) with EPA and DHA were significantly higher than those of the rice fieldeels fed diets(LO, SO, PL, PO) without EPA and DHA (P0.05). In general, the n-3PUFAs and appropriate n-6/n-3 ratiowere important for the growth of rice field eels. Soybean oil, linseed oil and pork lard were feasible lipid resources forreplace fish oil on growth of rice field eels and soybean oil is preferable lipid resources. The fatty acid (18:2n-6, 18:3n-3,ARA, EPA and DHA) profiles of liver, muscle and ovary reflected profiles of the corresponding dietary lipid sources.The rice field eel only possess the infirmatory ability to synthesize EPA and DHA from linolenic acid (18:3n-3) andARA from linoleic acid (18:2n-6) for the limitation in the capacity of fatty acid desaturation.