独活活性单体对中型指环虫的杀灭作用及其成分鉴定
EFFECT OF THE ACTIVE MONOMER FROM RADIX ANGELICAE PUBESCENTIS ON KILLING DACTYLOGYRUS INTERMEDIUS AND ITS ACTIVE COMPONENT IDENTIFICATION
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摘要: 利用硅胶柱层析和生物活性跟踪对独活乙醇提取物进行杀中型指环虫活性成分的分离纯化, 并测定活性成分在不同浓度条件下的离体生物活性, 通过薄层色谱(TLC)、紫外光谱(UV)、高效液相色谱(HPLC)、气质联用(GC-MS)对活性成分进行鉴定。结果表明: 从独活分离得到的F 和K1-2-3 两种流分均对中型指环虫具有不同程度的杀灭作用, 其中F 流分48h 内的完全杀灭中型指环虫的浓度为1.6 mg/L, 而K1-2-3 的使用浓度分别为5 mg/L 时, 其48h 内对中型指环虫的杀灭率为74.9%。经理化性质测定和光谱分析, F 流分和K1-2-3流分分别鉴定为蛇床子素和东莨菪内酯。Abstract: Activated components of parasiticidal Dactylogyrus intermedius extracted from Radix angelicae pubescentis were isolated and purificated by column chromatography and biologica l tracking in this study. The activity in vitro of activated components at different concentrations were determined. Then their chemical structures were identified by TLC, UV, HPLC and GC-MS. Observations implied that the killing effect of the components on D. intermedius at different concentrations were fraction Fand K1-2-3. Fraction F could reach to 100% parasiticidal rate when the concentration was 1.6 mg/L. As for K1-2-3, concentrations of 5 mg/L could reach to the parasiticidal rate of 74.9%. According to physico- chemical property examination and spectroanalysis, fraction F and K1-2-3 were identified as the osthole and scopoletine.
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