留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
王丹丽, 孙婷, 左迪, 王兰梅, 赵云龙. 红螯光壳螯虾白斑综合症血液病理学研究[J]. 水生生物学报, 2012, 36(3): 441-449. DOI: 10.3724/SP.J.1035.2012.00441
引用本文: 王丹丽, 孙婷, 左迪, 王兰梅, 赵云龙. 红螯光壳螯虾白斑综合症血液病理学研究[J]. 水生生物学报, 2012, 36(3): 441-449. DOI: 10.3724/SP.J.1035.2012.00441
WANG Dan-Li, SUN Ting, ZUO Di, WANG Lan-Mei, ZHAO Yun-Long. HAEMOLYMPH PATHOLOGICAL RESEARCH ON WHITE SPOT SYNDROME (WSS) OF CHERAX QUADRICARINATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(3): 441-449. DOI: 10.3724/SP.J.1035.2012.00441
Citation: WANG Dan-Li, SUN Ting, ZUO Di, WANG Lan-Mei, ZHAO Yun-Long. HAEMOLYMPH PATHOLOGICAL RESEARCH ON WHITE SPOT SYNDROME (WSS) OF CHERAX QUADRICARINATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(3): 441-449. DOI: 10.3724/SP.J.1035.2012.00441

红螯光壳螯虾白斑综合症血液病理学研究

HAEMOLYMPH PATHOLOGICAL RESEARCH ON WHITE SPOT SYNDROME (WSS) OF CHERAX QUADRICARINATUS

  • 摘要: 采用Wright-Geimsa 染色法和电镜技术对人工感染的红螯光壳螯虾(Cherax quadricarinatus)白斑综合症(White spot syndrome, WSS) 血液病理学进行了研究。结果显示: 患病螯虾血细胞总数、透明细胞(AH)数量极显著减少(P P P <0.01)。显微病理学变化主要表现为血涂片中血细胞明显减少, 病变、破损或解体的细胞增多, 至濒死期螯虾血液呈典型的溶血状态。超微病理学变化表现为血细胞受到了损伤。高尔基体变形、线粒体结构模糊破损; 核膜变形核固缩、细胞核高度异染色质化;濒临死亡的螯虾血细胞细胞器和染色质溶解, 胞浆水肿, 细胞溶解坏死。在患病螯虾的血细胞核中清晰可见WSSV 粒子。

     

    Abstract: White spot syndrome virus (WSSV) as the pathogenic agent caused acute and lethal infect ion of shrimp, resulting in mass economic losses to shrimp aquaculture industry all around the world especially in China. In recent years, the cultivation of redclaw crayfish (Cherax quadricarinatus) is developing, and the disease of C. quadricarinatus is one of the major factors in its culture and even causes redclaw crayfish to die. The viral disease was found in polyculture of redclaw crayfish with Penaeus vannamei To explore the pathogenic mechanism of C. quadricarinatus infected by WSSV (white spot syndrome virus), the pathological research in haemolymph on WSS of C. quadricarinatus infected by WSSV artificially was studied by means of Wright-Geimsa staining and electron microscope techniques. The results indicated that total haemocyte count in diseased shrimps was lower heavily than that in healthy shrimps (P6 cell/mL and 53.18×106 cell/mL respectively in 72h experimental shrimp and danger experimental shrimp, and was higher heavily than that in healthy shrimps (17.37×106 cell/mL, P P >0.05) after treated with WSSV-infected. The main micro-pathological changes of haemocyte showed that there was a decreasing hemocytes in haemolymph smear from diseased shrimps, in which the number of AH was lower heavily than that in healthy shrimps and the number of large granule hemocyte was higher than that in healthy shrimps. Synchronously broken and disintegrated cells could often be seen in the haemolymph smear, and contractive nucleus and anamorphic nuclear membrane in hyaline hemocyte, bubbles in denaturalized small granule hemocyte, pyknosis nucleus and bulgy cells in large granule hemocyte. There was a typical haemolysis in danger experimental shrimp. The ultra- micro-pathological changes showed that there were necrotic changes in every kind of haemocytes. Golgi apparatus was transmutative, mitochondria was damaged and incomplete in structure; Parts of nuclear membrane were anamorphic and parts of nucleus were contractive, while nucleus heterochromatinization in blood cells; Organelle and chromatin were dissolved, kytoplasm was dropsical in danger haemocytes, while cells were necrotic. There were clear WSSV in certain hemocyte nucleus of diseased shrimps. WSSV was a kind of baculovirus under transmission electron microscope (TEM), and the sizes of which were (99—118) nm × (189—321) nm. Its size and shape was consistent with WSSV which has been reported.

     

/

返回文章
返回