Abstract: This study was carried out using healthyCyprinus with the initial body weight of (33.52±0.17) g in a single recirculating system for 8 weeks. Diets with equal levels of protein (35 g/kg) and energy (17 kJ/g) were administered to the Cyprinus at concentrations of 0.0 % (control diet) and 0.5% (trial diet) Ala-Gln of dietary dry matter in five different feeding modes: continuous 8 weeks of control diet (group I or control), trial diet at an interval of 2 weeks (group II), trial diet at 4 weeks and then control diet at 4 weeks (group III), the contrary to group Ⅲ (group IV) and continuous 8-week trial diet (group V). The trail was conducted to investigate the effects of different feeding mode of Ala-Gln on growth, immunity and antioxidant in Cyprinus. Our results indicated that growth rates under all different feeding modes of Ala-Gln were significantly higher than that of the control group (P0.05). Growth rate of Ⅱ was higher than that of Ⅲ, Ⅳ,Ⅴgroups (P0.05), and growth rate of Ⅲ was higher than that ofⅤgroup (P0.05). Serum SOD of the Ⅳ and Ⅴgroups were significantly higher than the control group (P0.05), and serum SOD of the Ⅴgroup was significantly higher than those of Ⅱ and Ⅲ groups (P0.05). GSH-Px in hepatopancreas of different feeding modes were significantly higher than that of the control group (P0.05), Serum GSH-Px of Ⅳ and Ⅴgroups were higher than that of the control group (P0.05). LZM in the head kidneys of the Ala-Gln groups were higher than the control group, only group Ⅱ showed no significant difference (P0.05), LZM in the spleen of the groups Ⅳ and Ⅴ were significantly higher than that of the control group (P0.05). In the current experiment, we concluded that adding Ala-Gln could improve the growth, immunity and antioxidant levels of Cyprinus. There were significant differences of different feeding modes. Given economical efficiency and practicability, feeding mode with 2-week intervals were suggested in the practical aquaculture of Cyprinus.