克氏原螯虾源致病性豚鼠气单胞菌的分离及其生物学特性
ISOLATION AND BIOLOGICAL CHARACTERISTICS OF AN AEROMONASCAVIAE PATHOGEN FROM PROCAMBARUS CLARKII
-
摘要: 从患病的克氏原螯虾体内分离到一株致病菌L2M-A, 经生理生化鉴定和16S rDNA序列分析, 证实菌株L2M-A为豚鼠气单胞菌(Aeromonas caviae) (GenBank登录号: KF446251), 其16S rDNA序列与基因库中气单胞菌属菌株的16S rDNA序列有99%100%的同源性, 而且与豚鼠气单胞菌JXZ-3株(GenBank登录号: JF496552)的亲缘关系最近。此外, 菌株L2M-A在pH59内均能够生长良好, 最适生长温度为30℃, 最适生长转速为200 r/min, 但浓度6.25 g/mL的双氟沙星对其生长具有显著的抑制作用, 可作为防治用药的依据。Abstract: A pathogenic strain L2M-A was isolated from diseased Procambarus clarkii, and was identified as an Aeromonas caviae strain (GenBank accession No. KF446251) with biochemical and molecular approaches. The 16S rDNA sequence of this strain showed a homology of 99% to 100% with those of Aeromonas strains, and was closest related to that of A. caviae strain JXZ-3 (GenBank accession No: JF496552). We also found that the optimal growth conditions of strain L2M-A were pH5-9, temperature at 30℃, and spinning speed at 200 r/min. The growth of L2M-A could be significantly inhibited by difloxacin at the concentration of 6.25 g/mL. This finding could provide scientific guidance for the disease control.
下载: