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周继术, Bente E. Torstensen, Ingunn Stubhaug. 饲料中植物油替代鱼油对大西洋鲑肝细胞油酸跨膜吸收的影响[J]. 水生生物学报, 2014, 38(1): 121-128. DOI: 10.7541/2014.16
引用本文: 周继术, Bente E. Torstensen, Ingunn Stubhaug. 饲料中植物油替代鱼油对大西洋鲑肝细胞油酸跨膜吸收的影响[J]. 水生生物学报, 2014, 38(1): 121-128. DOI: 10.7541/2014.16
Zhou Ji-shu, Bente E. Torstensen, Ingunn Stubhaug. OLEIC ACID TRANS-MEMBRANE UPTAKE IN HEPATOCYTES OF ATLANTIC SALMON (SALMO SALAR L.) AND EFFECT OF REPLACING DIETARY FISH OIL WITH VEGETABLE OIL[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(1): 121-128. DOI: 10.7541/2014.16
Citation: Zhou Ji-shu, Bente E. Torstensen, Ingunn Stubhaug. OLEIC ACID TRANS-MEMBRANE UPTAKE IN HEPATOCYTES OF ATLANTIC SALMON (SALMO SALAR L.) AND EFFECT OF REPLACING DIETARY FISH OIL WITH VEGETABLE OIL[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(1): 121-128. DOI: 10.7541/2014.16

饲料中植物油替代鱼油对大西洋鲑肝细胞油酸跨膜吸收的影响

OLEIC ACID TRANS-MEMBRANE UPTAKE IN HEPATOCYTES OF ATLANTIC SALMON (SALMO SALAR L.) AND EFFECT OF REPLACING DIETARY FISH OIL WITH VEGETABLE OIL

  • 摘要: 以 1-14C油酸(oleic acid;18:1n-9,OA)为指示剂,研究了不同饲料油源饲喂下大西洋鲑肝细胞膜脂肪酸组成受到改变时该细胞对OA吸收的状况,以探讨植物油(Vegetable oil,VO)替代鱼油(Fish oil,FO)对大西洋鲑肝细胞脂肪酸跨膜吸收的影响,为大西洋鲑饲料中植物油替代鱼油的可行性提供理论依据。试验先以鱼油和大豆油为油源配制两种全价配合饲料,分别饲喂大西洋鲑幼鲑5个月,使其产生不同的脂肪酸组成。在饲养结束后,分离并培养试验鱼肝细胞,将细胞与1-14COA及37.5 mol/L OA(1/30,mol/mol,0.3 Ci/瓶)共孵育2h,收集并测定细胞内OA放射活性,再计算细胞内OA吸收量 nmol/(hmillion cells)。同时,试验采取RT-PCR方法测定了细胞脂肪酸运送蛋白(Fatty acid transport protein,FATP)、脂肪酸移位蛋白(Fatty acid translocase,FAT/CD36)的基因表达量。结果表明,FO和VO组肝细胞对OA吸收分别为(0.9240.258)及(0.8880.179)nmol/(hmillion cells),两组间无显著差异(P0.05)。RT-PCR的检测结果表明,FAT/CD36和FATP基因表达量在FO与VO两组间均无显著差异(P0.05)。结果表明,从植物油替代鱼油不影响大西洋鲑肝细胞对长链脂肪酸的跨膜吸收方面来看,大西洋鲑饲料中以植物油替代鱼油具有可行性。

     

    Abstract: Taking 1-14C OA (oleic acid;18:1n-9) as the indicator, the uptake of OA in Atlantic salmon (Salmo salar L.) hepatocyte with different membrane fatty acid composition induced by dietary fish oil (FO) and vegetable oil (VO) diets was determined to investigate the effect of replacing dietary fish oil with vegetable oil on fatty acid uptake and to provide the probability of replacing dietary fish oil with vegetable oil in Atlantic salmon diet. Atlantic salmon post smolt was fed diets containing either 100% FO or VO for 5 months to produce hepatocytes with typical FO and VO fatty acid composition, then OA uptake in isolated hepatocytes were studied by incubating with 1-14C OA and 37.5 mol/L OA (1/30, mol/mol, 0.3 Ci/flask) for 2h and by calculating the radioactivity in the cells. Meanwhile total RNA of the other same batch of FO and VO hepatocytes were extracted and the relative expression of FATP (fatty acid transport protein) and FAT/CD36 (fatty acid translocase) were determined by RT-PCR. The result showed that OA uptake in FO and VO was (0.9240.258) nmol/(hmillion cells) cells and (0.8880.179) nmol/(hmillion cells) respectively and there was no significant different between them (P0.05). The relative expression of FATP and FAT/CD36 were not significantly different between FO and VO hepatocytes too. The results indicate that by the same OA uptake between FO and VO hepatocytes, replacing dietary FO with VO was available in Atlantic salmon diet.

     

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