Abstract:
Grass carp reovirus (GCRV) is the main cause of severe outbreaks of hemorrhagic disease in fingerling and yearling grass carp. The virus outer capsid proteins, VP5 and VP7, have been shown to play critical roles in the invasion of host cells. In our study, prokaryotic-expressed VP7, VP5, and the N and C terminals of VP5 protein were used as targets to select the desired antibodies from phage-displayed scFv library. After 3 rounds of panning, we selected 7 antibodies against the prokaryotic-expressed antigens -- VP7, VP5, VP5N and VP5C. Furthermore, we found that 2 antibodies against VP7 out of the total 7 were able to recognize native GCRV particles. Our study should provide an insight into the interactions between GCRV and its host cells.
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