留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
李斌, 黄艳, 邵晨, 王宇. 铜离子急性胁迫对虎纹蛙肝脏中三羧酸循环及自由基代谢的影响[J]. 水生生物学报, 2015, 39(6): 1160-1168. DOI: 10.7541/2015.152
引用本文: 李斌, 黄艳, 邵晨, 王宇. 铜离子急性胁迫对虎纹蛙肝脏中三羧酸循环及自由基代谢的影响[J]. 水生生物学报, 2015, 39(6): 1160-1168. DOI: 10.7541/2015.152
LI Bin, HUANG Yan, SHAO Chen, WANG Yu. THE EFFECTS OF ACUTE COPPER STRESS ON TCA CYCLE AND FREE RADICAL METABOLISM IN THE LIVER OF HOPLOBATRACHUS CHINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(6): 1160-1168. DOI: 10.7541/2015.152
Citation: LI Bin, HUANG Yan, SHAO Chen, WANG Yu. THE EFFECTS OF ACUTE COPPER STRESS ON TCA CYCLE AND FREE RADICAL METABOLISM IN THE LIVER OF HOPLOBATRACHUS CHINENSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(6): 1160-1168. DOI: 10.7541/2015.152

铜离子急性胁迫对虎纹蛙肝脏中三羧酸循环及自由基代谢的影响

THE EFFECTS OF ACUTE COPPER STRESS ON TCA CYCLE AND FREE RADICAL METABOLISM IN THE LIVER OF HOPLOBATRACHUS CHINENSIS

  • 摘要: 为探明铜离子(Cu2+)对两栖动物肝脏线粒体中三羧酸(Tricarboxyl acid, TCA)循环及自由基代谢的毒理作用,采用静水暴露实验,研究了Cu2+不同浓度和不同暴露时间对虎纹蛙(Hoplobatrachus chinensis)肝脏线粒体中异柠檬酸脱氢酶(ICDHm)活性、-酮戊二酸脱氢酶(-KGDH)活性、抗超氧阴离子(anti-O2)活性、过氧化氢(H2O2)含量、抑制羟自由基(inhabit-OH)活性、一氧化氮(NO)含量以及一氧化氮合成酶(NOS)活性的影响。暴露实验共设置6个Cu2+浓度组(0.0、2.0、4.0、6.0、8.0和10.0 mg/L),分5个暴露时间(0、24h、48h、72h和96h)取材,对每个浓度的不同暴露时间分别取6个样本,测定TCA循环及自由基代谢的相关指标。结果显示,在TCA循环中随着Cu2+浓度的增加和暴露时间的延长,时间和浓度因素对ICDHm活性影响无显著性交互作用(P0.05),暴露时间的延长对ICDHm活性无显著性影响(P0.05),但随着Cu2+浓度的增加ICDHm活性逐渐减小;而时间和浓度因素对-KGDH活性影响有显著交互作用(P0.05),暴露处理后-KGDH活性下降,分别在24h和96h的4.0、6.0 mg/L时活性最低。在自由基代谢中,时间和浓度因素对抗O2活性、H2O2含量影响有显著交互作用(P0.05),而对抑制OH活性、NO含量、NOS活性的影响无显著性交互作用(P0.05)。不同时间随着Cu2+浓度的增加,抗O2活性均呈现出逐渐下降的趋势;实验处理后H2O2含量升高,在24h的6.0 mg/L时含量最大;随着暴露时间的延长和Cu2+浓度的增加抑制OH活性均逐渐降低;而NO含量和NOS活性的变化趋势基本相同,即随着Cu2+浓度的增加先增加后减少并趋近0浓度组,且都在6.0 mg/L时达到最大。研究结果表明急性Cu2+暴露对虎纹蛙肝脏线粒体中TCA循环及自由基代谢有显著的毒性作用。

     

    Abstract: In this study, we applied acute toxicity test to investigate the toxic effects of copper ion(Cu2+) on tricarbo-xylic acid cycle(TCA) and free radical metabolism in the mitochondria of the liver of Hoplobatrachus chinensis. We treated the animals with Cu2+ at different concentrations and for different exposure time, and tested the activities of TCA-related enzymes and free radicals. We found that although Cu2+ at high concentrations could reduce the activity of ICDHm, prolonged exposure time had no significant effect on ICDHm(P 0.05). We did not observe cross effects between the concentration of Cu2+ and the exposure time on the activity of ICDHm(P 0.05). However there were significant cross effects between the exposure time and the concentration of Cu2+ on the activity of -KGDH(P 0.05). The activity of -KGDH decreased when exposed to Cu2+, and the lowest activities were detected when the exposure times were 24h and 96h and concentrations were 4.0 and 6.0 mg/L. In terms of free radical metabolism, the exposure time and copper concentration had strong cross effects on the activities of anti-O2 and H2O2 (P 0.05), but not on the activity of inhibit-OH, the content of NO, and the activity of NOS. The activities of anti-O2 decreased along with the increase in the concentration of Cu2+. The content of H2O2 increased after the treatments and perked at 24h and 6.0 mg/L. There was a negative correlation between the activity of inhibit-OH and the exposure time and the concen-tration of Cu2+. Along with the increase in the concentration of Cu2+, the content of NO and the activity of NOS first increased and then decreased to the values of the control group, and the maximum values appeared in the 6.0 mg/L group. Our study demonstrated that the acute exposure of Cu2+ could have significant toxic effects on TCA cycle and free radical metabolism in the mitochondria of the liver of H. chinensis.

     

/

返回文章
返回