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蒋霞敏, 毛欣欣. 原绿球藻的营养组分分析及饵料效果研究[J]. 水生生物学报, 2015, 39(1): 164-172. DOI: 10.7541/2015.21
引用本文: 蒋霞敏, 毛欣欣. 原绿球藻的营养组分分析及饵料效果研究[J]. 水生生物学报, 2015, 39(1): 164-172. DOI: 10.7541/2015.21
JIANG Xia-Min, MAO Xin-Xin. The nutrition composition and bait effect of Prochlorococcus marinus[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(1): 164-172. DOI: 10.7541/2015.21
Citation: JIANG Xia-Min, MAO Xin-Xin. The nutrition composition and bait effect of Prochlorococcus marinus[J]. ACTA HYDROBIOLOGICA SINICA, 2015, 39(1): 164-172. DOI: 10.7541/2015.21

原绿球藻的营养组分分析及饵料效果研究

The nutrition composition and bait effect of Prochlorococcus marinus

  • 摘要: 为探究原绿球藻(Prochlorococcus marinus)的应用, 采用生化分析测定了其营养组分, 并进行了投喂蒙古裸腹溞和褶皱臂尾轮虫饵料效果试验. 结果显示: 原绿球藻干品中含粗蛋白42.32%, 粗脂肪11.47%, 碳水化合物15.11%和灰分17.48%. 含16种氨基酸, 总氨基酸含量为35.88 mg/g, 包括必需氨基酸8种 (占总氨基酸的37.54%); 粗脂肪中含13种脂肪酸, 其中不饱和脂肪酸8种(占粗脂肪总重的58.26%), 单不饱和脂肪酸(54.12%)含量较高, 而多不饱和脂肪酸含量较低(4.14%). 用原绿球藻投喂蒙古裸腹溞, 藻最佳浓度是400104 cells/mL, 溞世代净生殖率达4.33, 内禀增长率为0.23. 蒙古裸腹溞在用原绿球藻强化之后, 二十碳五烯酸(EPA)能有效富集, 浓度可达6.69%. 不同密度的原绿球藻饲喂对褶皱臂尾轮虫抱卵数和抱卵率均影响显著. 投喂藻密度在48-96h阶段以600104 cells/mL为佳, 而96-144h以400104 cells/mL为佳. 通过研究发现, 原绿球藻具有较好的营养价值和饵料效果, 但在蒙古裸腹溞和褶皱臂尾轮虫培养中, 仍建议与其他藻混合投喂.

     

    Abstract: The microalgae Prochlorococcus marinus has a high reproductive rate and is abundantly distributed in the natural environment. To investigate the potential commercial values of Prochlorococcus, here we analyzed its nutritional composition using biochemical assay, and evaluated its feeding efficacy for Moina mongolica and Brachionus plicatilis using one-way ANOVA. Our results showed that Prochlorococcus consisted of crude proteins (42.32% of the total dry weight), crude lipids (11.47%), carbonhydrate (15.11%) and ash content (17.48%). The crude proteins were composed of 18 types of amino acids that accounted for 35.88% of the dry weight. Eight types were essential amino acids (37.54%). Thirteen types of fatty acids were also identified in the crude lipids, including 8 types of unsaturated fatty acids (58.26%). The crude lipids were enriched with monounsaturated fatty acids (54.12%) and were low in polyunsaturated fatty acids (4.14%). When fed with Prochlorococcus at the optimal density of 400104 cells/mL, M. mongolica displayed the highest net reproductive rate of 4.33 and the intrinsic growth rate of 0.23, and the concentration of accumulated EPA in M. mongolica reached 6.69%. The feeding density of Prochlorococcus also showed significant impact on the egg production and the egg mass rate of B. plicatilis. The optimal density was 600104 cells/mL during 48-96h and 400104 cells/mL during 96-144h. In conclusion, Prochlorococcus may have high nutritional values and thus could be effective feeding bait. We suggest that it should be mixed with other microalgae when used as feed for M. mongolica and B. plicatilis.

     

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