Abstract: We induced the gynogenetic offspring of the excellent Yangtze River grass carp using UV inactivated sperms of blunt snout bream by inhibiting second polar body method with cold shock. There were gynogenetic grass carp and grass bream hybrids in the offspring. The size of gynogenetic grass carp was consistent with the grass carp, while the size of grass bream hybrids was between grass carp and blunt snout bream. Partec flow cytometry showed that the relative DNA content of grass carp and gynogenetic grass carp were 23.01 and 22.72, respectively, of which the two groups were close, and that the relative DNA content of high size offspring was 25.38 between the grass carp and blunt snout bream, which should be the grass bream hybrids. 17 microsatellite markers were selected to detect the genetic diversity of the grass carp group, the gynogenetic grass carp group and the grass bream hybrids. The results showed that 59 alleles were detected, of which 43.18 were effective alleles. The mean value of number on alleles (N_a) of the grass carp, the grass bream hybrids and the gynogenetic grass carp group were 3.57, 2.86 and 2.79, respectively; the mean value of the effective alleles were 2.93, 2.37 and 1.96, respectively; the mean value of expected heterozygosity (H_e) were 0.6502, 0.5573 and 0.3775, respectively; the average value of polymorphism information content (PIC) were 0.5738, 0.4649 and 0.3791, respectively. The genetic diversity of the gynogenetic grass carp decreased significantly, compared with the grass carp control, indicating that meiotic gynogenetic method could get high homozygous of gynogenetic grass individuals. We modeled the Microsatellite DNA fingerprintings of the different grass carp group and screened nine specific microsatellite markers. This study will provide the basic molecular genetics information of the excellent grass carp.