Abstract: The present study investigated the relationship of Myxobolus species from gills of Carassius auratus gibelio, Long Lake, Henan with other similar species such as Myxobolus honghuensis, Myxobolus ampullicapsulatus, Myxobo-lus pharynae, and Myxobolus wulii based on the comparative analysis of morphological characters, sequence similarity, genetic distance, mutation sites, GC content of 18S rDNA, and the phylogenic trees. The results indicated that the gill parasitic Myxobolus species from Carassius auratus gibelio, Long Lake, Henan was Myxobolus honghuensis, and no obvious host symptom was observed by this species. The further investigation showed that Myxobolus pharynae and Myxobolus honghuensis were similar in morphology and in sequence that reached 99%-100%, and that the genetic distance was only 0-0.0013, and that they had the same 44.31%, GC content with only two mutation sites. These re-sults indicated that these two species should the same species.