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冯硕恒, 蔡佐楠, 麦康森, 艾庆辉. 大黄鱼CCT基因的克隆及其表达量随稚鱼生长发育的变化[J]. 水生生物学报, 2016, 40(4): 752-757. DOI: 10.7541/2016.99
引用本文: 冯硕恒, 蔡佐楠, 麦康森, 艾庆辉. 大黄鱼CCT基因的克隆及其表达量随稚鱼生长发育的变化[J]. 水生生物学报, 2016, 40(4): 752-757. DOI: 10.7541/2016.99
FENG Shuo-Heng, CAI Zuo-nan, MAI Kang-sen, AI Qing-hui. MOLECULAR CLONING AND GENETIC ONTOGENY OF CCT DURING THE DEVELOPMENT OF LARGE YELLOW CROAKER LARVAE (LARIMICHTHYS CROCEA)[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(4): 752-757. DOI: 10.7541/2016.99
Citation: FENG Shuo-Heng, CAI Zuo-nan, MAI Kang-sen, AI Qing-hui. MOLECULAR CLONING AND GENETIC ONTOGENY OF CCT DURING THE DEVELOPMENT OF LARGE YELLOW CROAKER LARVAE (LARIMICHTHYS CROCEA)[J]. ACTA HYDROBIOLOGICA SINICA, 2016, 40(4): 752-757. DOI: 10.7541/2016.99

大黄鱼CCT基因的克隆及其表达量随稚鱼生长发育的变化

MOLECULAR CLONING AND GENETIC ONTOGENY OF CCT DURING THE DEVELOPMENT OF LARGE YELLOW CROAKER LARVAE (LARIMICHTHYS CROCEA)

  • 摘要: 研究旨在克隆大黄鱼磷脂酰胆碱合成关键基因磷脂酰胆碱胞苷转移酶 (CCT)基因全长, 并检测其表达量随稚鱼生长发育的变化。利用同源克隆技术和RACE技术从大黄鱼肝脏中成功扩增出CCT的全长。同时应用real-time PCR法检测不同日龄大黄鱼稚鱼CCT的表达变化。序列分析表明, CCT全长2419 bp(Genbank登录号: KF006239.1), 包括273 bp 的5'端非编码区, 1107 bp的开放阅读框, 1010 bp的3'端非编码区,共编码369个氨基酸。系统进化树分析表明, 相比其他物种, 大黄鱼CCT基因与红鳍东方鲀的亲缘关系较近。定量结果表明, 孵化后, 大黄鱼仔稚鱼CCT的表达量随日龄的变化先显著升高, 在15日龄时达到最大值,随后显著下降并趋于平稳, CCT基因表达量的变化趋势与大黄鱼稚鱼消化系统的发育密切相关。

     

    Abstract: In the present study, the full length of CCT (CTP: choline phosphate cytidylyltranferase) cDNA from large yellow croaker (Larimichthys crocea) was obtained by homology-based cloning and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed that the full length of CCT cDNA (GenBank accession No. KF006240.1) was 2419 bp, which consisted of 1107 bp open-reading frame (ORF) encoding 369 amino acids, a 273 bp 5'-untranslated region (5'UTR) and a 1010 bp 3'-untranslated region (3'UTR). Phylogenetic tree analysis showed that the CCT gene of large yellow croaker had a closer relationship with Takifugu rubripes than others. The expression of CCT increased significantly at first, and then decreased, and peaked at 15 day after hatching. The genetic ontogeny of CCT was related to the development of large yellow croaker digestive system.

     

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