留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
张成锋, 苏胜彦, 朱健, 石连玉. 富集优势基因型的后备亲本筛选以及相关分子标记的遗传效应分析[J]. 水生生物学报, 2017, 41(1): 79-85. DOI: 10.7541/2017.10
引用本文: 张成锋, 苏胜彦, 朱健, 石连玉. 富集优势基因型的后备亲本筛选以及相关分子标记的遗传效应分析[J]. 水生生物学报, 2017, 41(1): 79-85. DOI: 10.7541/2017.10
ZHANG Cheng-Feng, SU Sheng-Yan, ZHU Jian, SHI Lian-Yu. CANDIDATE PARENTS SELECTION CONTAINING MULTIPLE GENOTYPES WITH HIGHER PERFORMANCE AND CORRELATED MOLECULAR MARKERS' GENETIC EFFECTS ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(1): 79-85. DOI: 10.7541/2017.10
Citation: ZHANG Cheng-Feng, SU Sheng-Yan, ZHU Jian, SHI Lian-Yu. CANDIDATE PARENTS SELECTION CONTAINING MULTIPLE GENOTYPES WITH HIGHER PERFORMANCE AND CORRELATED MOLECULAR MARKERS' GENETIC EFFECTS ANALYSIS[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(1): 79-85. DOI: 10.7541/2017.10

富集优势基因型的后备亲本筛选以及相关分子标记的遗传效应分析

CANDIDATE PARENTS SELECTION CONTAINING MULTIPLE GENOTYPES WITH HIGHER PERFORMANCE AND CORRELATED MOLECULAR MARKERS' GENETIC EFFECTS ANALYSIS

  • 摘要: 选取黄河鲤新品系亲本和子代共450尾,5对微卫星引物和4个基因区段扩增引物开展基因型检测,并检测它们与体重的关联性以及分析相应的遗传效应。结果显示:Koi42和4个SNPs位点对体重有显著的影响,获得具有超过富集2个优势基因型的候选亲本共13尾,富集优势分子标记基因型的候选亲本生长性能优势明显。通过多元逐步回归分析,利用AIC最佳模型筛选到COⅠ626、D-Loop253和Koi42共3个位点,发现Koi42贡献率较大,经Fisher精确性检验,发现其与性别存在关联,其加性效应接近显著水平(P < 0.05)。检测的多个上位效应组分中,仅有D-Loop253和Koi42的加性效应间的互作达到显著水平。对遗传方差组分进行剖分发现,加性方差占11.4%,两个位点的加性效应构成的上位效应占到77.5%,因此这3个分子标记对体重的影响主要以上位效应为主,而且是两两加性效应的占比较大,可以推断出D-Loop 253和Koi42两个分子标记的上位效应起主要作用。综上所述结果提示Koi42及与其有互作的D-Loop253可用于黄河鲤新品系的标记辅助选择,可以开展多个分子标记的富集选择。

     

    Abstract: Marker assistant breeding, molecular breeding and genome wide breeding can shorten the generation interval of germplasm improvement and creation, speed up the selection response. Contrast with traditional best linear unbiased prediction (BLUP), they can achieve the higher genetic gain with the higher cost. Practically, together with BLUP, preselection of candidate individuals, low density array, they will become an optional choice under the consideration of selection cost and efficiency. So, in the present article, we focus on the effect of limited markers enrichment on the candidate population growth performance.e.g. body weight. For the body weight phenotype can not only be determined by one genotype, but also by multiple loci additive effect, dominant effect and epitasis. Thus, multiple loci explored needed to do variance components analysis and identify the specific genetic effect of these loci. A total of 450 fish (parents and their offspring) were collected, which were from Nanquan farm in Freshwater Fisheries Research Center in Chinese Fishery Academy. A total of 5 microstallites primers and 4 primers used for functional gene regional amplification were selected to observe the corresponded genotypes of such individuals. Correlation analysis between effective molecular markers and body weight of Huanghe carp new strain and genetic effect analysis of such markers were examined too in the present paper. The result showed that, microstallite Koi42 and 4 SNPs from the functional genes have significant effect on the carp's body weight. 13 candidate parents which have over two genotypes with higher body weight were developed. These individuals have better growth performance compared to that without these perfect genotypes. Further analysis by multiple step-wise regressions was conducted in order to make sure which markers can be implicated in practical breeding together effectively. COⅠ626, D-Loop 253 and Koi42 were left by both linear regression analysis and AIC criteria, where Koi42 has the highest contribution to the body weight variation among 3 markers. It is that Koi42 genotypes is related with sex by Fisher exact test. Genetic analysis supply the information Koi42 plays its role by additive effect (P < 0.1). Among many epitasis components, significant effect between D-Loop 253 additive effect and Koi42 additive effect was found. Through variance components partition analysis, additive variance account for 11.4%, and effect of epitasis between two loci had the higher percentage (77.5%). This told us 3 mar-kers listed above contribute to the body weight mainly by epitasis. All of these results illustrated that Koi42 with its interaction partner D-Loop 253 could be used to marker assistant breeding or multiple markers based coselection.

     

/

返回文章
返回