留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
何文栋, 朱葆华, 冯磊力, 常雅青, 杨官品, 潘克厚. 微拟球藻富油藻株筛选及柱状光生物反应器培养评价研究[J]. 水生生物学报, 2017, 41(5): 1112-1117. DOI: 10.7541/2017.139
引用本文: 何文栋, 朱葆华, 冯磊力, 常雅青, 杨官品, 潘克厚. 微拟球藻富油藻株筛选及柱状光生物反应器培养评价研究[J]. 水生生物学报, 2017, 41(5): 1112-1117. DOI: 10.7541/2017.139
HE Wen-Dong, ZHU Bao-Hua, FENG Lei-Li, CHANG Ya-Qing, YANG Guan-Pin, PAN Ke-Hou. SCREENING OF LIPID-RICH NANNOCHLOROPSIS OCEANICA MUTANTS AND EVALUATION USING A BUBBLE COLUMN PHOTOBIOREACTOR[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(5): 1112-1117. DOI: 10.7541/2017.139
Citation: HE Wen-Dong, ZHU Bao-Hua, FENG Lei-Li, CHANG Ya-Qing, YANG Guan-Pin, PAN Ke-Hou. SCREENING OF LIPID-RICH NANNOCHLOROPSIS OCEANICA MUTANTS AND EVALUATION USING A BUBBLE COLUMN PHOTOBIOREACTOR[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(5): 1112-1117. DOI: 10.7541/2017.139

微拟球藻富油藻株筛选及柱状光生物反应器培养评价研究

SCREENING OF LIPID-RICH NANNOCHLOROPSIS OCEANICA MUTANTS AND EVALUATION USING A BUBBLE COLUMN PHOTOBIOREACTOR

  • 摘要: 研究以亲脂性荧光染料BODIPY505/515和流式细胞仪为基础, 从多株诱变海洋微拟球藻(Nannochloropsis oceanica)中筛选到4株候选富油藻株(MT-1,2,3,4), 并利用柱状光生物反应器对诱变株的产油能力进行了综合评价。结果表明, 藻株筛选时最佳BODIPY505/515使用浓度为0.87 μg/mL, 染色时间为10min; 4株诱变株产油性能较野生株有较大提高, 其中MT-4油脂积累达到了干重的66%, 油脂产率比野生型藻株提高了45%, 达到了27.32 mg/(L·d)。4株诱变株的脂肪酸组成合适, 其中C16和C18之和占78%以上, 且主要以饱和脂肪酸和单不饱和脂肪酸为主; 多不饱和脂肪酸只占总脂肪酸的6%—8%, 非常适合生物柴油生产。研究提供了一种针对海洋微拟球藻富油藻株快速、有效的筛选方法, 并以此为基础筛选得到4株极具生物柴油生产潜力的候选藻株, 有望用于规模化生产。

     

    Abstract: After industrialization occurred, fossil fuel reserves decreased drastically, and environmental pollution caused by fossil fuel consumption became more serious. Thus, clean and renewable energy alternatives have recently attracted increasing amounts of attention. Microalgae have many advantages, such as a high growth rate, suitable fatty acid profiles and high lipid content, which makes them one of the most promising sources for renewable energy production. However, making a profit from biodiesel production based on microalgae is difficult because of the high cost. There are various strategies to reduce the cost of biodiesel production, such as breeding the best microalgae strains through chemical mutagenesis, which is one of the most important methods. Although this method is effective, extraordinarily heavy follow-up work impedes its promotion and application. Fortunately, flow cytometry and lipophilic fluorescent dyes (BODIPY505/515) provide the opportunity to screen for the best mutants using high throughput. In this study, multiple mutants of Nannochloropsis oceanica were obtained by using chemical mutation (nitrosoguanidine, NTG). We developed a method based on flow cytometry and BODIPY505/515, which can screen microalgal candidates using high throughput. Our results showed that the optimal concentration and staining time for BODIPY505/515 were 0.87 μg/mL and 10min, respectively. Based on this method, four outstanding microalgal strains were obtained. To verify the reliability of the screening method, we further investigated the performance of these four strains cultivated in a column photobioreactor. The results showed that lipid productivity of the four mutants was remarkably higher than that of the wild type, and the highest value was 27.32 mg/(L·d), demonstrating that this method is effective and feasible. To further evaluate the potential of candidates for biodiesel production, we investigated factors such as the fatty acid profiles, chlorophyll a, and PSII quantum efficiency. The results indicated that all of these four mutants possessed great potential for biodiesel production.

     

/

返回文章
返回