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叶元土, 吴萍, 蔡春芳, 吴代武, 罗琪刚, 何杰, 高敏敏, 周露阳, 王梦影, 汪冰, 张宝彤, 萧培珍. 灌喂氧化鱼油后黄颡鱼胃肠道黏膜黑色素细胞分化和黑色素合成途径基因的差异表达[J]. 水生生物学报, 2018, 42(1): 57-67. DOI: 10.7541/2018.008
引用本文: 叶元土, 吴萍, 蔡春芳, 吴代武, 罗琪刚, 何杰, 高敏敏, 周露阳, 王梦影, 汪冰, 张宝彤, 萧培珍. 灌喂氧化鱼油后黄颡鱼胃肠道黏膜黑色素细胞分化和黑色素合成途径基因的差异表达[J]. 水生生物学报, 2018, 42(1): 57-67. DOI: 10.7541/2018.008
Yuan-Tu YE, Ping WU, Chun-Fang CAI, Dai-Wu WU, Qi-Gang LUO, Jie HE, Min-Min GAO, Lu-Yang ZHOU, Meng-Ying WANG, Bing WANG, Bao-Tong ZHANG, Pei-Zhen XIAO. TRANSCRIPTOME ANALYSIS OF MELANOCYTE DIFFERENTIATION AND THE MELANIN SYNTHESIS METABOLIC PATHWAY IN THE GASTROINTESTINAL MUCOSA OF YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO) FED OXIDIZED FISH OIL[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(1): 57-67. DOI: 10.7541/2018.008
Citation: Yuan-Tu YE, Ping WU, Chun-Fang CAI, Dai-Wu WU, Qi-Gang LUO, Jie HE, Min-Min GAO, Lu-Yang ZHOU, Meng-Ying WANG, Bing WANG, Bao-Tong ZHANG, Pei-Zhen XIAO. TRANSCRIPTOME ANALYSIS OF MELANOCYTE DIFFERENTIATION AND THE MELANIN SYNTHESIS METABOLIC PATHWAY IN THE GASTROINTESTINAL MUCOSA OF YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO) FED OXIDIZED FISH OIL[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(1): 57-67. DOI: 10.7541/2018.008

灌喂氧化鱼油后黄颡鱼胃肠道黏膜黑色素细胞分化和黑色素合成途径基因的差异表达

TRANSCRIPTOME ANALYSIS OF MELANOCYTE DIFFERENTIATION AND THE MELANIN SYNTHESIS METABOLIC PATHWAY IN THE GASTROINTESTINAL MUCOSA OF YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO) FED OXIDIZED FISH OIL

  • 摘要: 以黄颡鱼(Pelteobagrus fulvidraco)为实验对象, 灌喂氧化鱼油、鱼油7d后, 提取胃肠道黏膜总RNA, 采用RNA-seq测序并做转录组分析, 分析了黑色素生物合成途径关键酶(酪氨酸酶)及其相关蛋白基因、黑素体运动的3个蛋白基因、α黑素细胞刺激激素途径和WNT/β-catenin、EDN3和EDNRB、KIT及其配体KITL3个信号通路的主要蛋白基因的差异表达活性。结果显示, 黄颡鱼胃肠道黏膜中存在黑色素细胞分化和发育过程、黑色素合成及其调控途径的代谢网络, 通过绘制代谢网络得到了关键性酶或蛋白质的基因信息。在灌喂氧化鱼油后, 控制黑色素合成途径主要基因的表达活性显著下调, 可能导致黑色素合成量的不足; α-MSH激素途径主要基因差异表达上调, 具备促进黑色素细胞分化和发育的调控基础; 而调控黑色素细胞分化和发育的3个信号通路主要基因也有差异表达。因此, 黄颡鱼受灌喂氧化鱼油的影响, 黑色素细胞分化和发育过程受到较大影响, 会影响到鱼体成熟的黑色素细胞的数量, 同时, 黑色素的生物合成量不足将导致引起黄颡鱼体色的变化。

     

    Abstract: Transcriptome analysis of yellow catfish (Pelteobagrus fulvidraco) by RNA-seq method extracting gastrointestinal mucosa Total RNA was studied in this paper. The fish were fed with oxidized fish oil and fish oil respectively for 7d. Differential expression of genes related to melanin synthesis, melanosomes movement, melanocyte stimulating hormone-α pathway and three signal pathways controlling melanocyte differentiation were analyzed. The results showed that there were metabolic networks of melanocyte differentiation and development, melanin synthesis and regulation in the gastrointestinal mucosa. The genetic information of key enzymes and protein of melanin synthesis were introduced with plotting metabolic networks graph. The melanin content was deficient because of the genes expression level were significantly down-regulation related to controlling melanin synthesis after fed with oxidized fish oil. While melanocyte differentiation and development were improved because of the genes expression level were significantly up-regulation related to melanocyte stimulating hormone-α pathway (α-MSH). In the same time, genes expression level of WNT/β-catenin, EDN3 / EDNRB and KIT/KITL signal pathways were different. In conclusion, feeding oxidized fish oil affected melanocyte differentiation and development and reduced melanocyte number which benefited to maturity in yellow catfish. Insufficient melanin biosynthesis also led to body color variation of yellow catfish.

     

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