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潘彦羽, 代嫣然, 王飞华, 梁威. 东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究[J]. 水生生物学报, 2018, 42(2): 406-415. DOI: 10.7541/2018.052
引用本文: 潘彦羽, 代嫣然, 王飞华, 梁威. 东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究[J]. 水生生物学报, 2018, 42(2): 406-415. DOI: 10.7541/2018.052
PAN Yan-Yu, DAI Yan-Ran, WANG Fei-Hua, LIANG Wei. ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING ARCHAEA AND AMMONIA-OXIDIZING BACTERIA IN THE SURFACE SEDIMENTS OF DONGHU LAKE[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(2): 406-415. DOI: 10.7541/2018.052
Citation: PAN Yan-Yu, DAI Yan-Ran, WANG Fei-Hua, LIANG Wei. ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING ARCHAEA AND AMMONIA-OXIDIZING BACTERIA IN THE SURFACE SEDIMENTS OF DONGHU LAKE[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(2): 406-415. DOI: 10.7541/2018.052

东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究

ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING ARCHAEA AND AMMONIA-OXIDIZING BACTERIA IN THE SURFACE SEDIMENTS OF DONGHU LAKE

  • 摘要: 研究通过高通量测序和荧光定量PCR等分子生物学分析方法, 以氨单加氧酶基因(amoA)为分子标记, 研究了东湖表层沉积物中AOA和AOB的群落多样性、丰度及其与环境因子的关系。结果表明, 东湖沉积物AOA主要为Nitrosopumilus, 其群落结构与沉积物中总氮含量显著相关, 而AOB主要为Nitrosomonas, 群落结构与沉积物中总有机碳和总磷显著相关。此外, 不同季节AOA丰度均高于AOB, 且沉积物AOA数量与温度呈显著负相关, 但AOB丰度变化不明显。东湖沉积物中AOA可能主导了氨氧化过程。

     

    Abstract: Ammonia oxidation is the rate-limiting step in nitrification. Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play important roles in ammonia oxidation. Sediments are important place for nitrogen circulation in lake. This study investigated the community diversity and abundance of AOA and AOB in the surface sediments of Donghu Lake and their relationships with environmental factors utilizing high-throughput sequencing and real-time quantitative PCR of ammonia monooxygenase (amoA) gene. The results showed that AOA and AOB were dominated by Nitrosopumilus and Nitrosomonas, respectively. There were significant correlations between the community structure of AOA and total nitrogen (TN) in sediments. AOB were significantly related to the contents of total organic carbon (TOC) and total phosphorus (TP). Additionally, AOA abundance was higher than that of AOB in the sediments. A significantly negative correlation between AOA abundance and temperature was detected, but there was no obvious change of AOB among the different seasons. These results implied that AOA could be the dominant driver in the ammonia oxidation in Donghu Lake.

     

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