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郭晓慧, 郭雅琼, 李扬. 海链藻属一新变种艾伦海链藻肋纹变种[J]. 水生生物学报, 2018, 42(4): 824-831. DOI: 10.7541/2018.101
引用本文: 郭晓慧, 郭雅琼, 李扬. 海链藻属一新变种艾伦海链藻肋纹变种[J]. 水生生物学报, 2018, 42(4): 824-831. DOI: 10.7541/2018.101
GUO Xiao-Hui, GUO Ya-Qiong, LI Yang. THALASSIOSIRA ALLENII VAR. STRIATA AS A NEW VARIETY IN THE GENUS THALASSIOSIRA CLEVE[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 824-831. DOI: 10.7541/2018.101
Citation: GUO Xiao-Hui, GUO Ya-Qiong, LI Yang. THALASSIOSIRA ALLENII VAR. STRIATA AS A NEW VARIETY IN THE GENUS THALASSIOSIRA CLEVE[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(4): 824-831. DOI: 10.7541/2018.101

海链藻属一新变种艾伦海链藻肋纹变种

THALASSIOSIRA ALLENII VAR. STRIATA AS A NEW VARIETY IN THE GENUS THALASSIOSIRA CLEVE

  • 摘要: 为了澄清海链藻属Thalassiosira的物种多样性, 采用毛细管复洗技术建立了单克隆培养株系。利用光镜和扫描电镜观察形态学特征, 并扩增其核糖体小亚基和大亚基序列, 用于分子系统树的构建。结合形态学和分子系统学数据, 发现艾伦海链藻株系之间存在一定的形态差异和遗传多样性。经过与原始文献的比对, 确认了艾伦海链藻原变种的特征, 并报道了该种的一个新变种艾伦海链藻肋纹变种Thalassiosira allenii var. striata X. H. Guo, Y. Q. Guo & Y. Li。该变种与原变种的形态特征基本相似, 区别在于壳面边缘具有肋纹结构, 原变种则无。基于核糖体小亚基和大亚基的系统树均显示, 肋纹变种与原变种聚在同一个分支上, 形成姐妹支(BPP>0.90), 表明两者之间具有最近的亲缘关系。2个变种的核糖体小亚基序列完全一致, 没有碱基差异。但用于分析的556个核糖体大亚基序列中, 两者存在11个差异碱基, 遗传距离为0.01。

     

    Abstract: Thalassiosira Cleve is one of the most speciose marine diatom genera, that has been globally distributed. To assess the diversity of the genus Thalassiosira species, monoclonal strains were isolated and established from Chinese coast. The morphology was observed by using a light microscope (LM) and a scanning electron microscope (SEM). Sequences of the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) were obtained to form a phylogenetic relationship. Among 14 established Thalassiosira allenii (T. allenii) Takano strains, morphological variation was found involving some with and without marginal ribs on the valve edge. After comparing with the original description of T. allenii, the strains without marginal ribs were assigned as a type of T. allenii, and those with marginal ribs were proposed as a new variety, T. allenii var. striata. Morphological divergence was supported by molecular data as well. On the phylogenetic trees inferred from both LSU and SSU of the nuclear rDNA, accordingly, T. allenii was clustered with var. striata, and formed as sister groups (Bayesian posterior probability (BPP) > 0.90), demonstrating their firm relationship. SSU sequences are totally identical, without any different base pair, between these two varieties. However, 11 different base pairs among 556 LSU base pair, which used for analysis, could be found, and divergence of distance among them is only 0.01.

     

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