留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
王纤纤, 曾本和, 刘乐乐, 杨瑞斌, 刘海平. 饲料蛋白质水平对异齿裂腹鱼生长、消化酶活性、非特异性免疫及蛋白质代谢反应的影响[J]. 水生生物学报, 2020, 44(4): 693-706. DOI: 10.7541/2020.084
引用本文: 王纤纤, 曾本和, 刘乐乐, 杨瑞斌, 刘海平. 饲料蛋白质水平对异齿裂腹鱼生长、消化酶活性、非特异性免疫及蛋白质代谢反应的影响[J]. 水生生物学报, 2020, 44(4): 693-706. DOI: 10.7541/2020.084
WANG Xian-Xian, ZENG Ben-He, LIU Le-Le, YANG Rui-Bin, LIU Hai-Ping. EFFECTS OF FEED PROTEIN LEVELS ON GROWTH, DIGESTIVE ENZYME ACTIVITIES, NON-SPECIFIC IMMUNITY AND PROTEIN METABOLISM OF SCHIZOTHORAX O’CONNORI[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(4): 693-706. DOI: 10.7541/2020.084
Citation: WANG Xian-Xian, ZENG Ben-He, LIU Le-Le, YANG Rui-Bin, LIU Hai-Ping. EFFECTS OF FEED PROTEIN LEVELS ON GROWTH, DIGESTIVE ENZYME ACTIVITIES, NON-SPECIFIC IMMUNITY AND PROTEIN METABOLISM OF SCHIZOTHORAX O’CONNORI[J]. ACTA HYDROBIOLOGICA SINICA, 2020, 44(4): 693-706. DOI: 10.7541/2020.084

饲料蛋白质水平对异齿裂腹鱼生长、消化酶活性、非特异性免疫及蛋白质代谢反应的影响

EFFECTS OF FEED PROTEIN LEVELS ON GROWTH, DIGESTIVE ENZYME ACTIVITIES, NON-SPECIFIC IMMUNITY AND PROTEIN METABOLISM OF SCHIZOTHORAX O’CONNORI

  • 摘要: 为研究饲料中不同蛋白质水平对异齿裂腹鱼(Schizothorax)生长、消化酶活性、非特异性免疫及蛋白质代谢反应的影响, 配制蛋白质水平为20.01%、25.00%、30.19%、35.24%、40.12%和45.10%的6种等脂等能的饲料。选取初始体重为(115.46±16.20) g的异齿裂腹鱼540尾, 随机分成6组, 每组3个重复, 每个重复30尾, 进行为期94d的饲养试验。结果表明: 随着饲料蛋白水平升高, 异齿裂腹鱼末重(FW)、增重率(WGR)、特定生长率(SGR)均先升高后降低, 且在蛋白水平为40.12%时最大, 与30.19%组差异不显著(P>0.05); 蛋白质效率(PER)、成活率(SR)均呈先升高后下降趋势, 在蛋白水平为25.00%时最大, 与30.19%组差异不显著(P>0.05); 摄食率(FR)先降低后趋于稳定, 在蛋白水平为20.01%时最高, 显著高于其余试验组(P<0.05); 饲料系数(FCR)和肝体比(HSI)均呈先降低后升高的变化趋势, 在蛋白质水平为40.12%时最低, 与35.24%组差异不显著(P>0.05); 全鱼粗蛋白质显著升高(P<0.05); 胰蛋白酶(TPS)、脂肪酶(LPS)、淀粉酶(AMS)活性变化趋势基本与SGRWGRPER相一致, 且TPS>LPS>AMS; 肝脏中超氧化物歧化酶(SOD)活性逐渐升高, 在45.10%组最高, 与30.19%—40.12%组差异不显著(P>0.05), 谷丙转氨酶(ALT)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性先升高后降低, ALT和ACP活性在35.24%组高、AKP活性在30.19%组最高, 但各试验组差异不显著(P>0.05); 血清中的尿素氮显著上升(P<0.05); 血清中的血氨在45.10%组最大, 显著高于25.00%组(P<0.05)。异齿裂腹鱼WGRSGRFCRPER与不同蛋白质水平分别进行二次回归分析得, 在试验条件下, 异齿裂腹鱼饲料中最佳蛋白质水平为31.20%—34.02%。

     

    Abstract: To evaluate the effects of dietary protein levels on the growth, digestive enzymes activities, non-specific immunity, as well as protein metabolism of Schizothorax o’connori, 6 equal fat and equal energy diets were formulated containing 20.01%, 25.00%, 30.19%, 35.24%, 40.12% and 45.10%, respectively, for a 94-day trial. A total of 540 Schizothorax o’connori with the average initial weight of (115.46±16.20) g were randomly distributed into six groups with three replicates per group, containing 30 Schizothorax o’connori per replicate. The results indicated that with the increase of dietary protein level, the final weight (FW), weight gain rate (WGR), specific growth rate (SGR) increased first and then decreased with the maximum level at 40.12% group that was not significantly different from that of 30.19% group (P>0.05). The protein efficiency ratio (PER) and survival rate (SR) increased first and then decreased with the maximum level in 25.00% group that was not significantly different from that of 30.19% group (P>0.05). The feeding ratio (FR) decreased first and then stabilized with the highest level in 20.01% group that was significantly higher than other groups (P<0.05). The feed conversion ratio (FCR) and hepaticsomatic index (HSI) decreased first and then increased with the lowest level in 40.12% group that were not significantly different from those of 35.24% group (P>0.05). Whole fish crude protein content increased significantly (P<0.05). The activity trends of trypsin (TPS), lipase (LPS) and amylase (AMS) were basically consistent with SGR, WGR and PER, and TPS > LPS > AMS. The activity of superoxide dismutase (SOD) in hepatopancreas increased with the maximum level in 45.10% group that was not significantly different from 30.19%—40.12% groups (P>0.05). Alanine transaminase (ALT), acid phosphatase (ACP) and alkaline phosphatase (AKP) increased first and then decreased with the highest ALT and ACP level in 35.24% group and the highest AKP in 30.19% group, and there were not significantly different among groups (P>0.05). Urea nitrogen increased significantly (P<0.05). The highest serum ammonia was in 45.10% group, which was significantly higher than 25.00% group (P<0.05). Based on the quadratic regression analysis of the WGR, SGR, FCR, PER and dietary protein levels, the optimal dietary protein level of Schizothorax o’connori was 31.20%—34.02% under the experimental condition.

     

/

返回文章
返回