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于哲, 吴莉芳, 李良, 朱瑞. 不同C/N生物絮团对洛氏鱥急性铜暴露保护作用[J]. 水生生物学报, 2021, 45(3): 601-608. DOI: 10.7541/2021.2020.046
引用本文: 于哲, 吴莉芳, 李良, 朱瑞. 不同C/N生物絮团对洛氏鱥急性铜暴露保护作用[J]. 水生生物学报, 2021, 45(3): 601-608. DOI: 10.7541/2021.2020.046
YU Zhe, WU Li-Fang, LI Liang, ZHU Rui. PROTECTIVE EFFECTS OF DIFFERENT C/N RATIOS FORMED BIOFLOCS ON ACUTE COPPER EXPOSURE OF RHYNCHOCYPRIS LAGOWSKII DYBOWSKI[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(3): 601-608. DOI: 10.7541/2021.2020.046
Citation: YU Zhe, WU Li-Fang, LI Liang, ZHU Rui. PROTECTIVE EFFECTS OF DIFFERENT C/N RATIOS FORMED BIOFLOCS ON ACUTE COPPER EXPOSURE OF RHYNCHOCYPRIS LAGOWSKII DYBOWSKI[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(3): 601-608. DOI: 10.7541/2021.2020.046

不同C/N生物絮团对洛氏鱥急性铜暴露保护作用

PROTECTIVE EFFECTS OF DIFFERENT C/N RATIOS FORMED BIOFLOCS ON ACUTE COPPER EXPOSURE OF RHYNCHOCYPRIS LAGOWSKII DYBOWSKI

  • 摘要: 实验旨在探索不同C/N生物絮团对急性铜暴露洛氏鱥免疫抑制、炎症反应与氧化应激的保护作用。挑选480尾洛氏鱥(Rhynchocypris lagowskii Dybowski)幼鱼(10±0.15) g, 随机分成4组, 每组3个重复, 每个重复40尾鱼。对照组饲喂商品料(C/N 10.8﹕1), 实验组选择葡萄糖为外添碳源调节C/N 15﹕1(Ⅱ组)、C/N 20﹕1(Ⅲ组)和C/N 25﹕1(Ⅳ组), 生长实验为56d, 之后进行为期96h的急性铜暴露胁迫实验。结果表明, 各实验组中免疫、抗氧化酶活性与炎症因子含量随着C/N的增加先升高后下降。其中, 与对照组相比, Ⅲ和Ⅳ组血清碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、溶菌酶(LSZ)、一氧化氮合酶(NOS)、补体C3、C4和免疫球蛋白M(IgM)水平显著升高(P<0.05), 而Ⅲ和Ⅳ组之间补体C3和C4水平差异不显著(P>0.05); Ⅲ和Ⅳ组血清过氧化氢酶(CAT)、抗坏血酸(ASA)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)与谷胱甘肽还原酶(GR)酶活性显著高于对照组(P<0.05), 而丙二醛(MDA)含量显著低于对照组(P<0.05)。同时Ⅱ组的SOD和CAT酶活性也有所升高, 且与对照组差异显著(P<0.05); Ⅲ和Ⅳ组血清肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)与白介素-6(IL-6)含量显著低于对照组(P<0.05), 且它们两组之间无显著差异(P>0.05)。然而, Ⅱ、Ⅲ和Ⅳ组白介素-2(IL-2)含量均显著高于对照组, 且第Ⅲ组含量最高。在实验条件下, 当C/N≥15: 1时, 生物絮团能有效地增强急性铜暴露下洛氏鱥的免疫与抗氧化酶活力, 且在C/N为20﹕1时效果最为显著。

     

    Abstract: This study combined BFT with waterborne copper to explore the protective effects of different C/N ratios formed bioflocs of R. lagowskii on immunosuppression, inflammation and oxidative stress in acute copper exposure. The results showed that with the increase of C/N ratio, the activities of antioxidation, immune enzymes and anti-inflammation factor of each group increased at first and then decreased. Compared with the control group, the levels of serum alkaline phosphatase (AKP), acid phosphatase (ACP), lysozyme (LSZ), nitric oxide synthase (NOS), complement C3, C4 and immunoglobulin M (IgM) increased significantly in Ⅲ and Ⅳ groups (P<0.05). However, there was no significant difference in complement C3 and C4 content between Ⅲ and Ⅳ groups (P>0.05). The activities of serum catalase (CAT), ascorbic acid (ASA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX) and glutathione reductase (GR) enzyme in Ⅲ and Ⅳ groups were significantly higher than those in the control (P<0.05), while the content of malondialdehyde (MDA) was significantly lower than that in the control group (P<0.05). At the same time, the activities of SOD and CAT in treatment Ⅱ were significantly higher than those in the control group (P<0.05). The serum levels of tumor necrosis factor-α (TNF-α), interleukin-1 β (IL-1β) and interleukin-6 (IL-6) in Ⅲ and Ⅳ groups were significantly lower than those in the control group (P<0.05) without significant difference between Ⅲ group and Ⅳ group (P>0.05). However, the content of interleukin-2 (IL-2) in Ⅱ, Ⅲ and Ⅳ groups were significantly higher than that of the control group (P<0.05) with the highest content in group Ⅲ. Overall, these findings suggest that bioflocs can effectively enhance the activities of immune and antioxidant enzymes under acute copper exposure when C/N≥15 with the most significant effect at a 20﹕1 C/N ratio. Therefore, this study provides a new idea for in-depth exploration of bioflocs, a new mitigation scheme for water environmental pollution and biological toxicity caused by heavy metals, and a theoretical basis for promoting the healthy and stable development of aquaculture.

     

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