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梅玲玉, 韩冬, 巫丽云, 郭伟, 刘昊昆, 金俊琰, 杨云霞, 朱晓鸣, 解绶启. 饲料不同水平淀粉对全养殖周期异育银鲫“中科3号”生长和糖代谢的影响[J]. 水生生物学报, 2021, 45(3): 557-565. DOI: 10.7541/2021.2020.051
引用本文: 梅玲玉, 韩冬, 巫丽云, 郭伟, 刘昊昆, 金俊琰, 杨云霞, 朱晓鸣, 解绶启. 饲料不同水平淀粉对全养殖周期异育银鲫“中科3号”生长和糖代谢的影响[J]. 水生生物学报, 2021, 45(3): 557-565. DOI: 10.7541/2021.2020.051
MEI Ling-Yu, HAN Dong, WU Li-Yun, GUO Wei, LIU Hao-Kun, JIN Jun-Yan, YANG Yun-Xia, ZHU Xiao-Ming, XIE Shou-Qi. A LONG-TERM CULTURE OF GIBEL CARP (CARASSIUS AURATUS GIBELIO) IN NET CAGES FOR 340 DAYS: EFFECTS OF DIETARY STARCH ON GROWTH AND GLUCOSE METABOLISM[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(3): 557-565. DOI: 10.7541/2021.2020.051
Citation: MEI Ling-Yu, HAN Dong, WU Li-Yun, GUO Wei, LIU Hao-Kun, JIN Jun-Yan, YANG Yun-Xia, ZHU Xiao-Ming, XIE Shou-Qi. A LONG-TERM CULTURE OF GIBEL CARP (CARASSIUS AURATUS GIBELIO) IN NET CAGES FOR 340 DAYS: EFFECTS OF DIETARY STARCH ON GROWTH AND GLUCOSE METABOLISM[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(3): 557-565. DOI: 10.7541/2021.2020.051

饲料不同水平淀粉对全养殖周期异育银鲫“中科3号”生长和糖代谢的影响

A LONG-TERM CULTURE OF GIBEL CARP (CARASSIUS AURATUS GIBELIO) IN NET CAGES FOR 340 DAYS: EFFECTS OF DIETARY STARCH ON GROWTH AND GLUCOSE METABOLISM

  • 摘要: 研究探讨了饲料淀粉对全养殖周期异育银鲫(Carassius auratus gibelio)生长性能和糖代谢的影响。实验以玉米淀粉为主要糖源, 分别设计淀粉水平为3% (S3)、13% (S13)、23% (S23)、33% (S33)和43% (S43)的5种等氮等脂饲料, 在长江故道江面网箱中饲养初始体重为(12.0±0.1) g的异育银鲫幼鱼340d, 期间根据鱼体养成阶段共取样5次, 生长阶段分为63d(D63, 幼鱼期)、110d(D110, 养成前期)、223d(D223, 越冬期)、275d(D275, 越冬后)和340d(D340, 养成中后期)。研究结果表明, 幼鱼期D63异育银鲫后肠淀粉酶活力较低且对饲料淀粉水平变化不敏感, 自养成前期D110之后鱼体后肠淀粉酶活力增强且随饲料淀粉水平的变化产生波动。幼鱼期D63异育银鲫glut2 mRNA相对表达量无显著差异, 其他阶段异育银鲫glut2 mRNA相对表达量水平随饲料淀粉水平升高而升高。各个生长阶段异育银鲫糖酵解的敏感度高于糖异生代谢, 幼鱼期D63与养成前期D110异育银鲫糖脂转化能力较强。幼鱼期D63异育银鲫适宜饲料淀粉水平为23%; 养成前期D110异育银鲫适宜饲料淀粉水平减为13%; 越冬后D275组异育银鲫整体代谢旺盛, 43%淀粉组生长最好; 而在养成中后期D340异育银鲫饲料淀粉需求则再次降到33%。研究结果表明, 异育银鲫在养殖周期的不同阶段对饲料淀粉的需求和利用存在显著的差异, 可以为异育银鲫不同养殖阶段特有饲料配方的设计提供支撑。

     

    Abstract: To study the effects of dietary carbohydrate on growth and glucose metabolism of gibel carp, 5 different diets with 3% (S3), 13% (S13), 23% (S23), 33% (S33) and 43% (S43 starch were formulated for a 340-day trail with 5 sampling times of day 63 (D63, the juveniles fish stage), day 110 (D110, the prophase cultivation period), day 223 (D223, overwintering stage), day 275 (D275, after overwintering stage) and day 340 (D340, the middle and late cultivation period). The results showed that gibel carp at different developmental stages had various starch utilization ability and glucose metabolism. The hindgut amylase activity and sensitiveness were low in the juveniles gibel carp and then increased from the prophase cultivation period. The activities of amylase in hindgut changed with the increased dietary starch level after D63. The GLUT2 mRNA level in gibel carp at D63 among groups were similar, while it increased with the increased dietary starch levels at D110, D275 and D340. The glycolysis was higher than gluconeogenesis in gibel carp at all stages. High glucose-induced lipogenesis was found in gibel carp at D63 and D110. The optimum dietary starch level was 23% for juvenile gibel carp at D63. The dietary starch requirement decreased to 13% for gibel carp at D113. After overwintering of D275, gibel carp showed a compensatory ability to utilize starch with the best growth performance in diet with 43% of dietary starch. At D340, gibel carp exhibited the best growth performance in 33% dietary starch group. The present results indicated that gibel have various abilities of the requirement and utilization of dietary starch at different developmental stages.

     

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