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罗帅, 刘真诚, 王粟, 谷思雨, 王雪艳, 姜传奇, 鲍少攀, 方涛, 缪炜, 熊杰. 基于宏基因组的保安湖浮游动物多样性研究[J]. 水生生物学报, 2022, 46(11): 1719-1729. DOI: 10.7541/2022.2021.0337
引用本文: 罗帅, 刘真诚, 王粟, 谷思雨, 王雪艳, 姜传奇, 鲍少攀, 方涛, 缪炜, 熊杰. 基于宏基因组的保安湖浮游动物多样性研究[J]. 水生生物学报, 2022, 46(11): 1719-1729. DOI: 10.7541/2022.2021.0337
LUO Shuai, LIU Zhen-Cheng, WANG Su, GU Si-Yu, WANG Xue-Yan, JIANG Chuan-Qi, BAO Shao-Pan, FANG Tao, MIAO Wei, XIONG Jie. ZOOPLANKTON DIVERSITY IN BAO’AN LAKE BASED ON METAGENOMIC APPROACH[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1719-1729. DOI: 10.7541/2022.2021.0337
Citation: LUO Shuai, LIU Zhen-Cheng, WANG Su, GU Si-Yu, WANG Xue-Yan, JIANG Chuan-Qi, BAO Shao-Pan, FANG Tao, MIAO Wei, XIONG Jie. ZOOPLANKTON DIVERSITY IN BAO’AN LAKE BASED ON METAGENOMIC APPROACH[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(11): 1719-1729. DOI: 10.7541/2022.2021.0337

基于宏基因组的保安湖浮游动物多样性研究

ZOOPLANKTON DIVERSITY IN BAO’AN LAKE BASED ON METAGENOMIC APPROACH

  • 摘要: 研究于2019年春、夏、秋、冬四季对保安湖进行了水样采集, 基于宏基因组测序, 在优化物种鉴定和丰度计算方法的基础上, 考察了保安湖浮游动物的多样性、群落结构及其影响因素。共鉴定到浮游动物OTU 374种, 其中原生动物282个; 枝角类45个; 桡足类26个; 轮虫21个。从季节来看, 夏、秋季保安湖的浮游动物多样性高; 从湖区来看, 肖四海和主湖区浮游动物多样性高。季节因素对保安湖浮游动物群落结构的影响高于湖区影响。保安湖营养状态为中营养型, 水体温度、叶绿素a是影响保安湖浮游动物群落结构的主要环境因子, 不同类群与环境因子相关性不同, 总体可分为5类。其中原生动物优势类群为混合营养的纤毛虫和丝足虫, 同硝氮、化学需氧量、温度有明显的相关性, 而枝角类和桡足类同环境因子的关系较为相似, 与溶氧、叶绿素a、正磷酸盐存在明显相关。研究利用宏基因组方法对保安湖浮游动物多样性开展了研究, 为从浮游动物这一角度来理解保安湖这一江湖阻隔型湖泊的生物多样性的变化提供了支撑。

     

    Abstract: The Bao’an Lake is a representative medium-sized lake among the Jianghan Lake Group. This lake, disconnecting to the river decades ago, have rich biological bait resources and good ecological environment. As important baits of fish, zooplankton is the key component of material and energy circulation in lake ecosystem, and good indicator of ecological health condition. The zooplankton diversity in Bao’an Lake was studied in the 1990s, however, the current status is unclear. In recent years, the rapid development of environmental DNA and metagenomic techniques provided a new approach to study the zooplankton diversity. In this study, water samples were collected from Bao’an Lake in the four seasons in 2019. Based on metagenomic sequencing, we optimized species identification and abundance estimation based on the metagenomic data, and subsequently analyzed the diversity of zooplankton in Bao’an Lake. The results showed that: (1) 374 OTUs of zooplankton were identified, including 282 protozoa, 45 cladocera, 26 copepoda and 21 rotifera, respectively; (2) For different seasons, high zooplankton diversity was found in summer and autumn; for different lake areas, high zooplankton diversity have been found in the Main-Lake-area and Xiao-Sihai-area; (3) Seasonal changes had greater effect on zooplankton community structure than different lake areas; (4) Temperature and chlorophyll-A were the main environmental factors affecting zooplankton community structure in Bao’an Lake. Five patterns have been found in correlations between the abundance of OTUs and environmental factors. Among them, the dominant groups of protozoa are Ciliophora and Cercozoa, their abundance correlate with the levels of nitrate nitrogen, chemical oxygen demand, and temperature, while Cladocera and Copepoda have similar relationship with environmental factors, and show significant correlations between chlorophyll-A and orthophosphate. In summary, we investigated the zooplankton diversity in Bao’an Lake using metagenomic approach, and provided the basic data for understanding the biodiversity changes in Bao’an Lake.

     

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