留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
罗相忠, 覃维敏, 梁宏伟, 沙航, 邹桂伟. 基于微卫星分析的长丰鲢种质资源遗传监测[J]. 水生生物学报. DOI: 10.7541/2022.2021.033
引用本文: 罗相忠, 覃维敏, 梁宏伟, 沙航, 邹桂伟. 基于微卫星分析的长丰鲢种质资源遗传监测[J]. 水生生物学报. DOI: 10.7541/2022.2021.033
LUO Xiang-Zhong, QIN Wei-Min, L IANG Hong-Wei, SHA Hang, ZOU Gui-Wei. GENETIC MONITORING OF CHANGFENG SILVER CARP BASE ON MICROSATELLITE[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2022.2021.033
Citation: LUO Xiang-Zhong, QIN Wei-Min, L IANG Hong-Wei, SHA Hang, ZOU Gui-Wei. GENETIC MONITORING OF CHANGFENG SILVER CARP BASE ON MICROSATELLITE[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2022.2021.033

基于微卫星分析的长丰鲢种质资源遗传监测

GENETIC MONITORING OF CHANGFENG SILVER CARP BASE ON MICROSATELLITE

  • 摘要: 长丰鲢(CF)为我国人工培育的鱼类新品种, 自推广应用以来取得了良好的效果。开展长丰鲢种质资源遗传监测, 对其优良性状保持具重要作用。本研究采用18对微卫星引物分析了鲢(L)和长丰鲢世代间(CF1、CF2和CF3)的遗传多样性和遗传结构。结果表明: 鲢遗传多样性指数高于长丰鲢, 遗传多样性也较长丰鲢丰富。而长丰鲢子代间CF1到CF3平均等位基因数(Na)从5.7222下降到5.0556; 平均有效等位基因数(Ne)从3.2551下降到3.1461; 平均观测杂合度(Ho)从0.6975下降到0.5407; 平均期望杂合度(He)从0.6422下降到0.6235; 多态信息含量(PIC)从0.5784下降到0.5609。CF1到CF3的遗传参数是逐渐下降, 遗传多样性逐渐降低, 但群体间遗传多样性仍较高。长丰鲢子代间Fst在0.0160—0.0315, 表明其群体已出现了遗传分化, 但分化程度较低。长丰鲢各世代间遗传距离逐渐增加, 遗传相似度逐渐减小。这表明经过连续3代利用, 长丰鲢CF1到CF3的遗传结构发生了改变, 遗传多样性呈下降趋势, 但遗传多样性水平仍较高。研究结果为长丰鲢进一步优良性状的维持提供了依据。

     

    Abstract: Changfeng silver carp (Hypophthalmichthys molitrix) (CF) is a new variety obtained by artificially selective breeding in China. Genetic monitoring the germplasm resources of Changfeng silver carp play an important role in maintaining its excellent traits. 18 microsatellite markers were used to analyze the genetic diversity and genetic structure in Changfeng silver carp (CF) populations. The results showed that the genetic diversity of silver carp (L) was higher than that of CF. The average number of alleles (Na) from CF1 to CF3 of CF offspring decreased from 5.7222 to 5.0556, and the average effective allele (Ne) were from 3.2551 to 3.1461. The average observed heterozygosity (Ho), the average expected heterozygosity (He), and the polymorphic information content (PIC) ranged from 0.6975 to 0.5407, 0.6422 to 0.6235, and 0.5784 to 0.5609, respectively. The Fst among the progeny of L and CF ranged from 0.0160 to 0.0315, indicating that the population of L has been genetically differentiated with low degree of differentiation. These results showed that the genetic structure of CF1 to CF3 exhibit slightly declining genetic diversity after three successive generations, whereas the genetic diversity was still abundant. Our study provides a basis for maintaining the genetic diversity of Changfeng silver carp.

     

/

返回文章
返回