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潘亚雄, 周俊, 张宇, 陶晋升, 潘佳琳, 唐昭阳, 樊轶为, 胡名广, 李慧菊, 张建社, 褚武英. 翘嘴鳜mtf-1表达特征分析及镉胁迫对其节律性表达的影响[J]. 水生生物学报, 2023, 47(2): 316-322. DOI: 10.7541/2023.2022.0131
引用本文: 潘亚雄, 周俊, 张宇, 陶晋升, 潘佳琳, 唐昭阳, 樊轶为, 胡名广, 李慧菊, 张建社, 褚武英. 翘嘴鳜mtf-1表达特征分析及镉胁迫对其节律性表达的影响[J]. 水生生物学报, 2023, 47(2): 316-322. DOI: 10.7541/2023.2022.0131
PAN Ya-Xiong, ZHOU Jun, ZHANG Yu, TAO Jin-Sheng, PAN Jia-Lin, TANG Zhao-Yang, FAN Yi-Wei, HU Ming-Guang, LI Hui-Ju, ZHANG Jian-She, CHU Wu-Ying. CHARACTERISTICS OF MTF-1 AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH SINIPERCA CHUATSI[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 316-322. DOI: 10.7541/2023.2022.0131
Citation: PAN Ya-Xiong, ZHOU Jun, ZHANG Yu, TAO Jin-Sheng, PAN Jia-Lin, TANG Zhao-Yang, FAN Yi-Wei, HU Ming-Guang, LI Hui-Ju, ZHANG Jian-She, CHU Wu-Ying. CHARACTERISTICS OF MTF-1 AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH SINIPERCA CHUATSI[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 316-322. DOI: 10.7541/2023.2022.0131

翘嘴鳜mtf-1表达特征分析及镉胁迫对其节律性表达的影响

CHARACTERISTICS OF MTF-1 AND CADMIUM STRESS ON RHYTHMIC EXPRESSION IN CHINESE PERCH SINIPERCA CHUATSI

  • 摘要: 为了研究翘嘴鳜(Siniperca chuatsi)金属调节转录因子-1(Metal-regulated transcription factor-1, mtf-1)序列特征、组织表达及重金属镉胁迫对其昼夜节律性表达的影响, 研究对翘嘴鳜mtf-1基因启动子顺式调控元件和序列特征, 以及该基因在不同组织中的表达模式进行了分析, 并对水体镉胁迫下翘嘴鳜脑组织中mtf-1基因表达昼夜节律性变化进行了研究。结果表明, 翘嘴鳜mtf-1启动子中存在锌指蛋白ZNF136、ZNF384和ZNF143结合位点, 表明其表达受金属离子的潜在调控, 另外, 翘嘴鳜mtf-1启动子中还发现核心生物钟基因RORβ结合位点, 表明mtf-1基因可能参与昼夜节律调控。基因结构域分析表明翘嘴鳜mtf-1基因存在一个高度保守的DNA结合域及一个不保守的反式激活域。mtf-1基因在翘嘴鳜不同组织中均有表达, 其中脑组织中表达量最高、肾脏其次。在自然条件下, 翘嘴鳜脑组织中mtf-1基因呈现昼低夜高的表达趋势, 其基因表达峰值相位为ZT 12.65h(一日中的黄昏时期), 这与翘嘴鳜昼伏夜出, 黄昏最活跃的生活习性相符。在水体镉胁迫下, 翘嘴鳜脑组织mtf-1基因昼夜都呈现持续低表达, 昼夜表达差异不显著, 表明水体镉胁迫能显著抑制翘嘴鳜mtf-1基因表达, 破坏鳜鱼脑组织mtf-1昼夜节律。

     

    Abstract: To study the characteristics of mtf-1 gene of Siniperca chuatsi and the effects of cadmium stress on the circadian rhythm of mtf-1 gene in brain, we analyzed cis-regulatory elements of mtf-1 gene promoter, sequence characteristics and tissues expression patterns of mtf-1 gene, and explored the circadian rhythm changes of mtf-1 gene expression in brain of Siniperca chuatsi under cadmium stress. The results showed that there were binding sites of zinc finger proteins including ZNF136, ZNF384 and ZNF143 in the mtf-1 promoter of S. chuatsi, indicating that their expression is potentially regulated by metal ions. In addition, RORβ binding sites of the core clock gene were also found in the mtf-1 promoter of S. chuatsi, indicating that the mtf-1 gene is potentially involved in circadian rhythm regulation. The analysis of function domain indicated that mtf-1 gene has a highly conserved DNA binding domain and a non-conserved trans-activation domain. mtf-1 gene is expressed in different tissues of S. chuatsi, among which the highest expression level is found in brain tissue, followed by kidney. Under natural conditions, mtf-1 gene showed a trend of high expression at night and low expression in the brain at day. The expression peak phase occurs at ZT 12.65h (nightfall), which is consistent with its life habits. Under cadmium stress, the mtf-1 gene in the brain of S. chuatsi presented continuous low expression level, and with no significant difference between day and night, indicating that the mtf-1 gene expression in the brain of S. chuatsi could be significantly inhibited by cadmium, and the circadian rhythm of mtf-1 in the brain tissue of S. chuatsi is damaged.

     

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