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孙秋凤, 柳梅梅, 何杰, 张敏, 陶贤继, 吴旭干. 三疣梭子蟹卵巢发育过程中主要类胡萝卜素组成变化及其与抗氧化性能的关系[J]. 水生生物学报, 2023, 47(5): 713-722. DOI: 10.7541/2023.2022.0152
引用本文: 孙秋凤, 柳梅梅, 何杰, 张敏, 陶贤继, 吴旭干. 三疣梭子蟹卵巢发育过程中主要类胡萝卜素组成变化及其与抗氧化性能的关系[J]. 水生生物学报, 2023, 47(5): 713-722. DOI: 10.7541/2023.2022.0152
SUN Qiu-Feng, LIU Mei-Mei, HE Jie, ZHANG Min, TAO Xian-Ji, WU Xu-Gan. CHANGES IN CAROTENOID COMPOSITION AND ANTIOXIDANT CAPACITY OF PORTUNUS TRITUBERCULATUS DURING OVARIAN DEVELOPMENT[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(5): 713-722. DOI: 10.7541/2023.2022.0152
Citation: SUN Qiu-Feng, LIU Mei-Mei, HE Jie, ZHANG Min, TAO Xian-Ji, WU Xu-Gan. CHANGES IN CAROTENOID COMPOSITION AND ANTIOXIDANT CAPACITY OF PORTUNUS TRITUBERCULATUS DURING OVARIAN DEVELOPMENT[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(5): 713-722. DOI: 10.7541/2023.2022.0152

三疣梭子蟹卵巢发育过程中主要类胡萝卜素组成变化及其与抗氧化性能的关系

CHANGES IN CAROTENOID COMPOSITION AND ANTIOXIDANT CAPACITY OF PORTUNUS TRITUBERCULATUS DURING OVARIAN DEVELOPMENT

  • 摘要: 实验研究了三疣梭子蟹(Portunus trituberculatus)卵巢发育过程中(Ⅰ—V期)组织色泽、类胡萝卜素组成与含量、抗氧化和非特异性免疫指标的变化规律, 进一步探讨了肝胰腺中类胡萝卜素含量变化与抗氧化指标的关系。结果表明: (1)卵巢发育期间, 卵巢指数(GSI)显著增加(P<0.05), 肝胰腺指数(HSI)整体呈先升后降的趋势; 卵巢的红度(a*)值和黄度(b*)值、肝胰腺的亮度(L*)值和b*值均呈显著上升趋势, 但卵巢中L*值呈下降趋势。(2)在卵巢发育过程中, 卵巢中的总类胡萝卜素、虾青素、叶黄素和β-胡萝卜素含量均为先升后降的趋势, 虾青素含量在Ⅳ期最高; 肝胰腺中的虾青素含量呈上升趋势, 而β-胡萝卜素含量为显著下降趋势; 内表皮中的总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量均呈现下降趋势; 就不同组织中类胡萝卜素含量而言, 内表皮中虾青素含量最高。卵巢和肝胰腺的L*值与总类胡萝卜素含量呈显著负相关, 卵巢的a*值和b*值均与总类胡萝卜素、虾青素、叶黄素、海胆烯酮和β-胡萝卜素含量呈显著正相关。肝胰腺的a*值与各类胡萝卜素含量无显著相关性, b*值仅与虾青素含量呈显著正相关。(3)肝胰腺中Ⅰ—V期T-AOC呈显著下降趋势(P<0.05), 而SOD、CAT和GPX均为“先上升后下降”趋势; 血淋巴中T-AOC呈现“高-低-高-低”的变化趋势, 而SOD和GPX为“先上升后下降”趋势, CAT和MDA为“低-高-低-高”的变化趋势; 卵巢发育过程中肝胰腺中ACP、ALP和NO整体呈下降趋势, 而血淋巴中ACP、ALP和Hc在Ⅰ—Ⅳ期为上升趋势, V期略有下降。(4)相关性分析表明, 肝胰腺中虾青素含量与T-AOC呈显著负相关, 叶黄素含量与GPX呈显著正相关(P<0.01), 其余类胡萝卜素含量与抗氧化指标均无显著相关性。综上, 在三疣梭子蟹卵巢发育过程中, 卵巢中总类胡萝卜素、虾青素和β-胡萝卜素的含量增加主要发生在Ⅱ—Ⅳ期, 内表皮中大部分类胡萝卜素呈显著下降趋势, 肝胰腺中仅虾青素呈上升趋势。

     

    Abstract: Female Portunus trituberculatus at different stages of ovarian development (stage Ⅰ—Ⅴ) were investigated for their color, carotenoids contents, antioxidant and non-specific immune parameters. The results indicated that: (1) the gonadosomatic index (GSI) increased significantly (P<0.05), while the hepatosomatic index (HSI) rose slightly, then declined gradually. The redness (a*) and yellowness (b*) values in ovaries showed an increasing trend, as did lightness (L*) and b* values in hepatopancreas, but L* values in ovaries showed a decreasing trend. (2) Total carotenoids, astaxanthin, zeaxanthin and β-carotene content in ovaries showed an increasing trend followed by a decreasing trend, with the highest astaxanthin content in stage IV. Astaxanthin content in hepatopancreas was on the rise, while β-carotene was on a significant decline. Regarding the content of carotenoids in different tissues, the epithelium contained the highest amount of astaxanthin. L* values in the ovary and hepatopancreas exhibited a significant negative correlation with total carotenoids, and a* and b* values in the ovary both showed a significant positive correlation with total carotenoids, astaxanthin, luteolin, echinenone and β-carotene. There was no significant correlation between a* values and various carotenoids in hepatopancreas, and b* values only showed a significant positive correlation with astaxanthin. (3) For antioxidant indexes, T-AOC in hepatopancreas showed a significant decrease in stage Ⅰ—Ⅴ (P<0.05), while SOD, CAT and GPX showed an “increasing and then decreasing” trend. The T-AOC in hemolymph showed a trend of “high-low-high-low”. SOD and GPX showed an increasing trend and then decreasing trend, while CAT and MDA showed a “low-high-low-high” trend. In terms of immune indexes, ACP, ALP and NO in the hepatopancreas showed an overall decreasing trend during ovarian development, while ACP, ALP and Hc in the hemolymph showed an increasing trend in stage Ⅰ—Ⅳ and a slight decrease in stage Ⅴ. (4) Correlation analysis showed that astaxanthin content in hepatopancreas was significantly negatively correlated with T-AOC, lutein content was significantly positively correlated with GPX (P<0.01), and no significant correlation was found between the other carotenoids content and antioxidant indexes. In conclusion, the increase of total carotenoids, astaxanthin and β-carotene in the ovaries of P. trituberculatus occurred mainly in stage Ⅱ—Ⅳ, while most of the carotenoids in the epithelium showed a significant decreasing trend and only astaxanthin in the hepatopancreas showed an increasing trend.

     

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