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邹峰余, 韩亚康, 罗智, 赵涛, 徐杰杰, 谭肖英. 棉籽粕和菜籽粕替代部分鱼粉对黄颡鱼肉质及肌肉发育相关基因表达的影响[J]. 水生生物学报, 2023, 47(2): 227-234. DOI: 10.7541/2023.2022.0231
引用本文: 邹峰余, 韩亚康, 罗智, 赵涛, 徐杰杰, 谭肖英. 棉籽粕和菜籽粕替代部分鱼粉对黄颡鱼肉质及肌肉发育相关基因表达的影响[J]. 水生生物学报, 2023, 47(2): 227-234. DOI: 10.7541/2023.2022.0231
ZOU Feng-Yu, HAN Ya-Kang, LUO Zhi, ZHAO Tao, XU Jie-Jie, TAN Xiao-Ying. REPLACEMENT OF DIETARY FISH MEAL BY RAPESEED MEAL AND COTTONSEED MEAL ON MUSCLE TEXTURE, HEALTH AND EXPRESSION OF MUSCLE-RELATED GENES IN YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 227-234. DOI: 10.7541/2023.2022.0231
Citation: ZOU Feng-Yu, HAN Ya-Kang, LUO Zhi, ZHAO Tao, XU Jie-Jie, TAN Xiao-Ying. REPLACEMENT OF DIETARY FISH MEAL BY RAPESEED MEAL AND COTTONSEED MEAL ON MUSCLE TEXTURE, HEALTH AND EXPRESSION OF MUSCLE-RELATED GENES IN YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 227-234. DOI: 10.7541/2023.2022.0231

棉籽粕和菜籽粕替代部分鱼粉对黄颡鱼肉质及肌肉发育相关基因表达的影响

REPLACEMENT OF DIETARY FISH MEAL BY RAPESEED MEAL AND COTTONSEED MEAL ON MUSCLE TEXTURE, HEALTH AND EXPRESSION OF MUSCLE-RELATED GENES IN YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)

  • 摘要: 文章旨在研究菜籽粕与棉籽粕复合替代饲料鱼粉对黄颡鱼(Pelteobagrus fulvidraco)肌肉品质及肌纤维发育相关基因表达的影响。实验设计了5组等氮等能的饲料, 以含鱼粉25%的处理组为对照饲料(FM), 以菜籽粕与棉籽粕3﹕2混合后分别替代其中的10%、20%、30%和40%鱼粉, 设置了RM10、RM20、RM30和RM40 四个试验组。使用初始体质量为(2.38±0.10) g的黄颡鱼幼鱼, 分别用上述5种饲料饲喂10周。试验表明, 与对照组相比, 菜粕棉粕混合替代水平达到20%及以上时黄颡鱼肌肉中的粗蛋白与粗脂肪显著降低, 而水分和灰分则无显著差异; 肌肉中的羟脯氨酸含量呈现下降的趋势; 肌纤维中直径≥50 μm的纤维数量随着替代水平达到20%后不断减小, 而≤20 μm的纤维数量则呈现上升趋势; 植物蛋白源的替代也会对黄颡鱼的肌肉质构产生一定的影响。此外, 肌肉纤维发育相关基因myodmyogmrf4的mRNA水平随着替代水平上升而逐渐升高, 而myf5mstn的基因表达在各组间无显著差异。TOR通路相关基因中的akttors6k1等基因随着替代水平升高而下降, 4e-bp1则随着替代水平升高而上升。T-SOD和CAT的活性、及总抗氧化能力(T-AOC)随替代水平升高而下降, MDA水平随替代水平达到20%后发生显著性上升。研究表明, 以菜籽粕和棉籽粕复合替代黄颡鱼幼鱼饲料中超过10%的鱼粉时, 会损害黄颡鱼的肌肉生长及肌肉品质。

     

    Abstract: This article aims to study the effect of rapeseed meal and cottonseed meal on the muscle quality and the expression of genes related to muscle fiber development after replacing part of the fish meal in the diets of yellow catfish (Pelteobagrus fulvidraco). Five groups of equal nitrogen and equal energy diets were designed in the experiment. The group containing 25% fish meal was used as the control diet (FM), and rapeseed meal and cotton meal were mixed 3﹕2 to replace 10%, 20%, 30% and 40% fish meal, and then set up four test groups as RM10, RM20, RM30 and RM40, respectively. The juvenile yellow catfish with an initial body weight of (2.38±0.02) g were fed with the above five diets for 8 weeks. The experiment showed that, compared with the fishmeal group, as the replacement level exceeded 10%, the crude protein and crude fat in the muscle of yellow catfish decreased significantly when the replacement level increased, but there was no significant difference in moisture and ash. In addition, the content of hydroxyproline in muscle also showed a downward trend. Among the muscle fibers of yellow catfish, the number with a diameter of ≥50 μm decreased when the replacement level reached 10%, while the number of fibers with a diameter of ≤20 μm showed an upward trend. At the same time, the replacement of plant protein sources will also have a certain impact on the muscle texture of yellow catfish. In addition, the mRNA levels of muscle fiber development-related genes myod, myog, and mrf4 gradually increased as the replacement level reached 10%, while the gene expression of myf5 and mstn did not show significance between the groups. The expression of TOR and S6K1 decreased as the replacement level increased, and the expression of 4E-BP1 increased as the replacement level increased. Therefore, this study show that use of the rapeseed meal and cottonseed meal to replace more than 10% of the fish meal in the diet of yellow catfish juveniles will damage the muscle growth and muscle quality.

     

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