留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
傅建军, 龚雅婷, 朱文彬, 强俊, 张林兵, 王兰梅, 罗明坤, 董在杰. 大口黑鲈微卫星多重PCR体系及3个群体的遗传分析[J]. 水生生物学报, 2023, 47(9): 1514-1522. DOI: 10.7541/2023.2022.0296
引用本文: 傅建军, 龚雅婷, 朱文彬, 强俊, 张林兵, 王兰梅, 罗明坤, 董在杰. 大口黑鲈微卫星多重PCR体系及3个群体的遗传分析[J]. 水生生物学报, 2023, 47(9): 1514-1522. DOI: 10.7541/2023.2022.0296
FU Jian-Jun, GONG Ya-Ting, ZHU Wen-Bin, QIANG Jun, ZHANG Lin-Bing, WANG Lan-Mei, LUO Ming-Kun, DONG Zai-Jie. MULTIPLEX PCR SETS OF MICROSATELLITES AND THEIR APPLICATION IN GENETIC ANALYSES FOR THREE POPULATIONS OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1514-1522. DOI: 10.7541/2023.2022.0296
Citation: FU Jian-Jun, GONG Ya-Ting, ZHU Wen-Bin, QIANG Jun, ZHANG Lin-Bing, WANG Lan-Mei, LUO Ming-Kun, DONG Zai-Jie. MULTIPLEX PCR SETS OF MICROSATELLITES AND THEIR APPLICATION IN GENETIC ANALYSES FOR THREE POPULATIONS OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(9): 1514-1522. DOI: 10.7541/2023.2022.0296

大口黑鲈微卫星多重PCR体系及3个群体的遗传分析

MULTIPLEX PCR SETS OF MICROSATELLITES AND THEIR APPLICATION IN GENETIC ANALYSES FOR THREE POPULATIONS OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES)

  • 摘要: 为开展大口黑鲈(Micropterus salmoides)种质评估和遗传分析提供便捷的研究手段, 试验筛选了扩增效果好、具有多态性的18个微卫星位点, 构建了6组3重PCR体系。随后将多重PCR体系应用于大口黑鲈3个群体(美国群体USA、“优鲈1号”YLO和杂交子代HYB)的遗传分析。结果显示, 各位点的等位基因数(Na)、有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)和多态性信息含量(PIC)分别介于3—14、1.622—5.841、0.333—0.806、0.385—0.833和0.361—0.810, 其均值分别为7.722、3.056、0.577、0.626和0.579。剔除2个偏离Hardy-Weinberg平衡位点的分析显示, YLO的遗传多样性水平最低, HYB的平均观测杂合度最高(Ho=0.743), 其余多态性指标均在USA中呈现最高值。USA与YLO间的Nei’s遗传距离最远(0.362), HYB与USA和YLO间的Nei’s遗传距离分别为0.112和0.179。两两群体间均存在极显著的遗传分化(P<0.01), 其中USA与YLO间遗传分化水平最高(FST=0.209)。基于等位基因频率的遗传结构分析结果显示, USA和YLO具有相对独立的遗传结构, 而HYB呈现出不同的遗传来源。主成分判别分析的散点图显示, 不同群体来源的个体能获得较有效区分, HYB分布介于USA和YLO的区域之间。研究表明, 试验构建的微卫星多重PCR体系, 涉及位点具有较高多态性, 并能有效应用于大口黑鲈的群体遗传分析, 可为其遗传育种研究提供技术支持。

     

    Abstract: Largemouth bass (Micropterus salmoides) has been introduced approximately for forty years, and it becomes an economically important cultured fish in China. However, there is a lack of natural population supply in breeding and a risk of genetic degradation in the utilization progress. The imbalance between large industrial demand and shortage of improved varieties of M. salmoides are arisen in China. Genetic evolution is the baseline for genetic improvement and selection breeding programs. The microsatellites with multiplex PCR method is popularity applied for genetic analyses in fish species. In this study, we developed six multiplex PCR panels based on 18 polymorphic microsatellites, and subsequently used in genetic analyses for three populations of M. salmoides, i.e., original population of American (USA), “Youlu No. 1” variety (YLO), and their hybrid population (HYB). The results showed that, the parameters of number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content (PIC) for 18 loci were ranged from 3 to 14, 1.622 to 5.841, 0.333 to 0.806, 0.361 to 0.810, mean with 7.722, 3.056, 0.577 and 0.579, respectively. Excluding two loci deviated from Hardy-Weinberg equilibrium, the lowest values of all diversity parameters were observed in YLO, while the highest Ho was detected in HYB (0.743), and the highest values of other diversity parameters were detected in USA. The farthest Nei’s genetic distance was evaluated between USA and YLO (0.362); meanwhile, similar Nei’s genetic distances were evaluated between HYB with other two populations (i.e., 0.112 and 0.179 for USA and YLO, respectively). Extremely significant genetic differentiations were revealed among all pairwise populations (P<0.01), and the highest fixation index was detected between USA and YLO (FST=0.209). According to the genetic structure analysis based on the allele frequency, relatively independent genetic structures were presented within USA and YLO, while admixture in genetic structure was generally showed in HYB. Additionally, the distinct distributions of individuals from three populations were visualized by using scatter plots of discriminant analysis of principal component (DAPC), and HYB showed with interval distribution between USA and YLO. In brief, six multiplex PCR panels of 18 microsatellites were developed firstly in M. salmoides, relatively high polymorphisms were detected among these loci, and the effective application in genetic analyses was evaluated in this study. That provided valuable tools for genetic and breeding researches in M. salmoides.

     

/

返回文章
返回